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Fragmentation characteristics of neutral N-linked glycans using a MALDI-TOF/TOF tandem mass spectrometer.
Anal Chem. 2004 Apr 15; 76(8):2343-54.AC

Abstract

The fragmentation characteristics of native and permethylated oligosaccharides using a matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight tandem mass spectrometer are described. The use of two MALDI matrixes, alpha-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB), is shown to control the nature and extent of fragmentation observed in collision-induced dissociation experiments on synthetic oligosaccharides. CHCA promotes the occurrence of glycosidic cleavages, whereas DHB promotes a wide range of fragmentations. These latter fragmentations include glycosidic cleavages, cross-ring cleavages, and the formation of "internal" cleavage ions, which are derived from elimination of substituents from around the pyranose ring. This extensive fragmentation is shown to facilitate the detailed structural characterization of high-mannose and hybrid-type N-glycans purified from avidin. Importantly, the cross-ring fragments reveal linkage information, unambiguously define antennae substitutions, and differentiate isomeric glycoforms.

Authors+Show Affiliations

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK. es287@cam.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15080747

Citation

Stephens, Elaine, et al. "Fragmentation Characteristics of Neutral N-linked Glycans Using a MALDI-TOF/TOF Tandem Mass Spectrometer." Analytical Chemistry, vol. 76, no. 8, 2004, pp. 2343-54.
Stephens E, Maslen SL, Green LG, et al. Fragmentation characteristics of neutral N-linked glycans using a MALDI-TOF/TOF tandem mass spectrometer. Anal Chem. 2004;76(8):2343-54.
Stephens, E., Maslen, S. L., Green, L. G., & Williams, D. H. (2004). Fragmentation characteristics of neutral N-linked glycans using a MALDI-TOF/TOF tandem mass spectrometer. Analytical Chemistry, 76(8), 2343-54.
Stephens E, et al. Fragmentation Characteristics of Neutral N-linked Glycans Using a MALDI-TOF/TOF Tandem Mass Spectrometer. Anal Chem. 2004 Apr 15;76(8):2343-54. PubMed PMID: 15080747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fragmentation characteristics of neutral N-linked glycans using a MALDI-TOF/TOF tandem mass spectrometer. AU - Stephens,Elaine, AU - Maslen,Sarah L, AU - Green,Luke G, AU - Williams,Dudley H, PY - 2004/4/15/pubmed PY - 2005/2/3/medline PY - 2004/4/15/entrez SP - 2343 EP - 54 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 8 N2 - The fragmentation characteristics of native and permethylated oligosaccharides using a matrix-assisted laser desorption/ionization (MALDI) time-of-flight/time-of-flight tandem mass spectrometer are described. The use of two MALDI matrixes, alpha-cyano-4-hydroxycinnamic acid (CHCA) and 2,5-dihydroxybenzoic acid (DHB), is shown to control the nature and extent of fragmentation observed in collision-induced dissociation experiments on synthetic oligosaccharides. CHCA promotes the occurrence of glycosidic cleavages, whereas DHB promotes a wide range of fragmentations. These latter fragmentations include glycosidic cleavages, cross-ring cleavages, and the formation of "internal" cleavage ions, which are derived from elimination of substituents from around the pyranose ring. This extensive fragmentation is shown to facilitate the detailed structural characterization of high-mannose and hybrid-type N-glycans purified from avidin. Importantly, the cross-ring fragments reveal linkage information, unambiguously define antennae substitutions, and differentiate isomeric glycoforms. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15080747/Fragmentation_characteristics_of_neutral_N_linked_glycans_using_a_MALDI_TOF/TOF_tandem_mass_spectrometer_ L2 - https://doi.org/10.1021/ac030333p DB - PRIME DP - Unbound Medicine ER -