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The Saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination.
Genetics. 2004 Mar; 166(3):1187-97.G

Abstract

Haploid Saccharomyces can change mating type through HO-endonuclease cleavage of an expressor locus, MAT, followed by gene conversion using one of two repository loci, HML or HMR, as donor. The mating type of a cell dictates which repository locus is used as donor, with a cells using HML and alpha cells using HMR. This preference is established in part by RE, a locus on the left arm of chromosome III that activates the surrounding region, including HML, for recombination in a cells, an activity suppressed by alpha 2 protein in alpha cells. We have examined the ability of RE to stimulate different forms of interchromosomal recombination. We found that RE exerted an effect on interchromosomal mating-type switching and on intrachromosomal homologous recombination but not on interchromosomal homologous recombination. Also, even in the absence of RE, MAT alpha still influenced donor preference in interchromosomal mating-type switching, supporting a role of alpha 2 in donor preference independent of RE. These results suggest a model in which RE affects competition between productive and nonproductive recombination outcomes. In interchromosome gene conversion, RE enhances both productive and nonproductive pathways, whereas in intrachromosomal gene conversion and mating-type switching, RE enhances only the productive pathway.

Authors+Show Affiliations

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15082540

Citation

Houston, Peter, et al. "The Saccharomyces Cerevisiae Recombination Enhancer Biases Recombination During Interchromosomal Mating-type Switching but Not in Interchromosomal Homologous Recombination." Genetics, vol. 166, no. 3, 2004, pp. 1187-97.
Houston P, Simon PJ, Broach JR. The Saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination. Genetics. 2004;166(3):1187-97.
Houston, P., Simon, P. J., & Broach, J. R. (2004). The Saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination. Genetics, 166(3), 1187-97.
Houston P, Simon PJ, Broach JR. The Saccharomyces Cerevisiae Recombination Enhancer Biases Recombination During Interchromosomal Mating-type Switching but Not in Interchromosomal Homologous Recombination. Genetics. 2004;166(3):1187-97. PubMed PMID: 15082540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination. AU - Houston,Peter, AU - Simon,Peter J, AU - Broach,James R, PY - 2004/4/15/pubmed PY - 2004/11/13/medline PY - 2004/4/15/entrez SP - 1187 EP - 97 JF - Genetics JO - Genetics VL - 166 IS - 3 N2 - Haploid Saccharomyces can change mating type through HO-endonuclease cleavage of an expressor locus, MAT, followed by gene conversion using one of two repository loci, HML or HMR, as donor. The mating type of a cell dictates which repository locus is used as donor, with a cells using HML and alpha cells using HMR. This preference is established in part by RE, a locus on the left arm of chromosome III that activates the surrounding region, including HML, for recombination in a cells, an activity suppressed by alpha 2 protein in alpha cells. We have examined the ability of RE to stimulate different forms of interchromosomal recombination. We found that RE exerted an effect on interchromosomal mating-type switching and on intrachromosomal homologous recombination but not on interchromosomal homologous recombination. Also, even in the absence of RE, MAT alpha still influenced donor preference in interchromosomal mating-type switching, supporting a role of alpha 2 in donor preference independent of RE. These results suggest a model in which RE affects competition between productive and nonproductive recombination outcomes. In interchromosome gene conversion, RE enhances both productive and nonproductive pathways, whereas in intrachromosomal gene conversion and mating-type switching, RE enhances only the productive pathway. SN - 0016-6731 UR - https://www.unboundmedicine.com/medline/citation/15082540/The_Saccharomyces_cerevisiae_recombination_enhancer_biases_recombination_during_interchromosomal_mating_type_switching_but_not_in_interchromosomal_homologous_recombination_ DB - PRIME DP - Unbound Medicine ER -