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Intronic sequence with both negative and positive effects on the regulation of alternative transcripts of the chicken beta tropomyosin transcripts.
Nucleic Acids Res. 1992 Aug 11; 20(15):3987-92.NA

Abstract

The chicken beta tropomyosin gene generates three major transcripts by alternative splicing. A pair of internal exons are spliced in a mutually exclusive manner and their utilisation is developmentally regulated. Exon 6A and exon 6B are used respectively in myoblasts and myotubes during the process of differentiation of muscle cells. We have previously reported that, in myoblasts, exon 6B is skipped because of a negative regulation which involves intron as well as exon sequences. In this report, we describe a previously uncharacterized intronic element which is involved in the regulation of the splicing of both exons 6A and 6B. This cis-element is localized 37nt downstream of exon 6A and is approximately 30nt long. Its deletion, as well as modification of its sequence, results in the activation of the use of exon 6B and, at the same time, in the inhibition of the use of exon 6A. The mechanisms by which this region could act are further discussed.

Authors+Show Affiliations

Pasteur Institut, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1508684

Citation

Balvay, L, et al. "Intronic Sequence With Both Negative and Positive Effects On the Regulation of Alternative Transcripts of the Chicken Beta Tropomyosin Transcripts." Nucleic Acids Research, vol. 20, no. 15, 1992, pp. 3987-92.
Balvay L, Libri D, Gallego M, et al. Intronic sequence with both negative and positive effects on the regulation of alternative transcripts of the chicken beta tropomyosin transcripts. Nucleic Acids Res. 1992;20(15):3987-92.
Balvay, L., Libri, D., Gallego, M., & Fiszman, M. Y. (1992). Intronic sequence with both negative and positive effects on the regulation of alternative transcripts of the chicken beta tropomyosin transcripts. Nucleic Acids Research, 20(15), 3987-92.
Balvay L, et al. Intronic Sequence With Both Negative and Positive Effects On the Regulation of Alternative Transcripts of the Chicken Beta Tropomyosin Transcripts. Nucleic Acids Res. 1992 Aug 11;20(15):3987-92. PubMed PMID: 1508684.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intronic sequence with both negative and positive effects on the regulation of alternative transcripts of the chicken beta tropomyosin transcripts. AU - Balvay,L, AU - Libri,D, AU - Gallego,M, AU - Fiszman,M Y, PY - 1992/8/11/pubmed PY - 1992/8/11/medline PY - 1992/8/11/entrez SP - 3987 EP - 92 JF - Nucleic acids research JO - Nucleic Acids Res VL - 20 IS - 15 N2 - The chicken beta tropomyosin gene generates three major transcripts by alternative splicing. A pair of internal exons are spliced in a mutually exclusive manner and their utilisation is developmentally regulated. Exon 6A and exon 6B are used respectively in myoblasts and myotubes during the process of differentiation of muscle cells. We have previously reported that, in myoblasts, exon 6B is skipped because of a negative regulation which involves intron as well as exon sequences. In this report, we describe a previously uncharacterized intronic element which is involved in the regulation of the splicing of both exons 6A and 6B. This cis-element is localized 37nt downstream of exon 6A and is approximately 30nt long. Its deletion, as well as modification of its sequence, results in the activation of the use of exon 6B and, at the same time, in the inhibition of the use of exon 6A. The mechanisms by which this region could act are further discussed. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/1508684/Intronic_sequence_with_both_negative_and_positive_effects_on_the_regulation_of_alternative_transcripts_of_the_chicken_beta_tropomyosin_transcripts_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/20.15.3987 DB - PRIME DP - Unbound Medicine ER -