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Occurrence of copper resistant mutants in the toxic cyanobacteria Microcystis aeruginosa: characterisation and future implications in the use of copper sulphate as algaecide.
Water Res. 2004 Apr; 38(8):2207-13.WR

Abstract

Freshwater toxic cyanobacteria are an increasing problem to human and animal health. Control of cyanobacteria in water supply reservoirs involves the use of algaecides, such as copper sulphate, usually in a repetitive way. Repercussions of recurrent algaecide treatments on cyanobacteria population dynamics remain still unknown. We studied the adaptation of cyanobacteria to lethal doses of copper sulphate by using Microcystis aeruginosa as an experimental model. A fluctuation analysis demonstrated that copper-resistant cells arise by spontaneous mutations that occur randomly prior to exposition to copper sulphate. The rate of spontaneous mutation from copper sensitivity to resistance was 1.76 x 10(-6) mutants per cell division. Resistant mutants exhibited a diminished fitness in the absence of copper sulphate, but only these variants were able to grow at Cu(2+) concentrations higher than 5.8 microM. In addition, copper-resistant cells were significantly smaller than wild-type ones. Warnings on the long-term consequences of repetitive algaecide treatments in water supplies are suggested.

Authors+Show Affiliations

García-Villada L
Dpto. Producción Animal (Genética), Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, Madrid E-28040, Spain.
Rico M
No affiliation info available
Altamirano MM
No affiliation info available
Sánchez-Martín L
No affiliation info available
López-Rodas V
No affiliation info available
Costas E
No affiliation info available

MeSH

Bacterial ToxinsCopperCopper SulfateDrug Resistance, MicrobialEukaryotaMicrocystisMutationWater MicrobiologyWater Purification

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15087203

Citation

García-Villada, Libertad, et al. "Occurrence of Copper Resistant Mutants in the Toxic Cyanobacteria Microcystis Aeruginosa: Characterisation and Future Implications in the Use of Copper Sulphate as Algaecide." Water Research, vol. 38, no. 8, 2004, pp. 2207-13.
García-Villada L, Rico M, Altamirano MM, et al. Occurrence of copper resistant mutants in the toxic cyanobacteria Microcystis aeruginosa: characterisation and future implications in the use of copper sulphate as algaecide. Water Res. 2004;38(8):2207-13.
García-Villada, L., Rico, M., Altamirano, M. M., Sánchez-Martín, L., López-Rodas, V., & Costas, E. (2004). Occurrence of copper resistant mutants in the toxic cyanobacteria Microcystis aeruginosa: characterisation and future implications in the use of copper sulphate as algaecide. Water Research, 38(8), 2207-13.
García-Villada L, et al. Occurrence of Copper Resistant Mutants in the Toxic Cyanobacteria Microcystis Aeruginosa: Characterisation and Future Implications in the Use of Copper Sulphate as Algaecide. Water Res. 2004;38(8):2207-13. PubMed PMID: 15087203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of copper resistant mutants in the toxic cyanobacteria Microcystis aeruginosa: characterisation and future implications in the use of copper sulphate as algaecide. AU - García-Villada,Libertad, AU - Rico,Marcos, AU - Altamirano,M María, AU - Sánchez-Martín,Laura, AU - López-Rodas,Victoria, AU - Costas,Eduardo, PY - 2003/09/29/received PY - 2003/11/27/revised PY - 2004/01/29/accepted PY - 2004/4/17/pubmed PY - 2004/11/17/medline PY - 2004/4/17/entrez SP - 2207 EP - 13 JF - Water research JO - Water Res VL - 38 IS - 8 N2 - Freshwater toxic cyanobacteria are an increasing problem to human and animal health. Control of cyanobacteria in water supply reservoirs involves the use of algaecides, such as copper sulphate, usually in a repetitive way. Repercussions of recurrent algaecide treatments on cyanobacteria population dynamics remain still unknown. We studied the adaptation of cyanobacteria to lethal doses of copper sulphate by using Microcystis aeruginosa as an experimental model. A fluctuation analysis demonstrated that copper-resistant cells arise by spontaneous mutations that occur randomly prior to exposition to copper sulphate. The rate of spontaneous mutation from copper sensitivity to resistance was 1.76 x 10(-6) mutants per cell division. Resistant mutants exhibited a diminished fitness in the absence of copper sulphate, but only these variants were able to grow at Cu(2+) concentrations higher than 5.8 microM. In addition, copper-resistant cells were significantly smaller than wild-type ones. Warnings on the long-term consequences of repetitive algaecide treatments in water supplies are suggested. SN - 0043-1354 UR - https://www.unboundmedicine.com/medline/citation/15087203/Occurrence_of_copper_resistant_mutants_in_the_toxic_cyanobacteria_Microcystis_aeruginosa:_characterisation_and_future_implications_in_the_use_of_copper_sulphate_as_algaecide_ DB - PRIME DP - Unbound Medicine ER -