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Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion.
Eur J Neurosci. 2004 Apr; 19(8):2123-31.EJ

Abstract

We investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury. The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve-muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficialis and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function.

Authors+Show Affiliations

Department of Anatomy, Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15090039

Citation

Gu, Huai-Yu, et al. "Survival, Regeneration and Functional Recovery of Motoneurons in Adult Rats By Reimplantation of Ventral Root Following Spinal Root Avulsion." The European Journal of Neuroscience, vol. 19, no. 8, 2004, pp. 2123-31.
Gu HY, Chai H, Zhang JY, et al. Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion. Eur J Neurosci. 2004;19(8):2123-31.
Gu, H. Y., Chai, H., Zhang, J. Y., Yao, Z. B., Zhou, L. H., Wong, W. M., Bruce, I., & Wu, W. T. (2004). Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion. The European Journal of Neuroscience, 19(8), 2123-31.
Gu HY, et al. Survival, Regeneration and Functional Recovery of Motoneurons in Adult Rats By Reimplantation of Ventral Root Following Spinal Root Avulsion. Eur J Neurosci. 2004;19(8):2123-31. PubMed PMID: 15090039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion. AU - Gu,Huai-Yu, AU - Chai,Hong, AU - Zhang,Jian-Yi, AU - Yao,Zhi-Bin, AU - Zhou,Li-Hua, AU - Wong,Wai-Man, AU - Bruce,Iain, AU - Wu,Wu-Tian, PY - 2004/4/20/pubmed PY - 2004/6/23/medline PY - 2004/4/20/entrez SP - 2123 EP - 31 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 19 IS - 8 N2 - We investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury. The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve-muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficialis and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/15090039/Survival_regeneration_and_functional_recovery_of_motoneurons_in_adult_rats_by_reimplantation_of_ventral_root_following_spinal_root_avulsion_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0953-816X&date=2004&volume=19&issue=8&spage=2123 DB - PRIME DP - Unbound Medicine ER -