Tags

Type your tag names separated by a space and hit enter

Large-scale in vitro expansion of polyclonal human CD4(+)CD25high regulatory T cells.
Blood. 2004 Aug 01; 104(3):895-903.Blood

Abstract

CD4(+)CD25+ regulatory T (Treg) cells are pivotal for the maintenance of self-tolerance, and their adoptive transfer gives protection from autoimmune diseases and pathogenic alloresponses after solid organ or bone marrow transplantation in murine model systems. In vitro, human CD4(+)CD25+ Treg cells display phenotypic and functional characteristics similar to those of murine CD4(+)CD25+ Treg cells: namely, hyporesponsiveness to T-cell receptor (TCR) stimulation and suppression of CD25- T cells. Thus far, the detailed characterization and potential clinical application of human CD4(+)CD25+ Treg cells have been hampered by their paucity in peripheral blood and the lack of appropriate expansion protocols. Here we describe the up to 40 000-fold expansion of highly purified human CD4(+)CD25high T cells in vitro through the use of artificial antigen-presenting cells for repeated stimulation via CD3 and CD28 in the presence of high-dose interleukin 2 (IL-2). Expanded CD4(+)CD25high T cells were polyclonal, maintained their phenotype, exceeded the suppressive activity of freshly isolated CD4(+)CD25high T cells, and maintained expression of the lymph node homing receptors L-selectin (CD62L) and CCR7. The ability to rapidly expand human CD4(+)CD25high Treg cells on a large scale will not only facilitate their further exploration but also accelerate their potential clinical application in T cell-mediated diseases and transplantation medicine.

Authors+Show Affiliations

Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15090447

Citation

Hoffmann, Petra, et al. "Large-scale in Vitro Expansion of Polyclonal Human CD4(+)CD25high Regulatory T Cells." Blood, vol. 104, no. 3, 2004, pp. 895-903.
Hoffmann P, Eder R, Kunz-Schughart LA, et al. Large-scale in vitro expansion of polyclonal human CD4(+)CD25high regulatory T cells. Blood. 2004;104(3):895-903.
Hoffmann, P., Eder, R., Kunz-Schughart, L. A., Andreesen, R., & Edinger, M. (2004). Large-scale in vitro expansion of polyclonal human CD4(+)CD25high regulatory T cells. Blood, 104(3), 895-903.
Hoffmann P, et al. Large-scale in Vitro Expansion of Polyclonal Human CD4(+)CD25high Regulatory T Cells. Blood. 2004 Aug 1;104(3):895-903. PubMed PMID: 15090447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large-scale in vitro expansion of polyclonal human CD4(+)CD25high regulatory T cells. AU - Hoffmann,Petra, AU - Eder,Ruediger, AU - Kunz-Schughart,Leoni A, AU - Andreesen,Reinhard, AU - Edinger,Matthias, Y1 - 2004/04/15/ PY - 2004/4/20/pubmed PY - 2004/8/31/medline PY - 2004/4/20/entrez SP - 895 EP - 903 JF - Blood JO - Blood VL - 104 IS - 3 N2 - CD4(+)CD25+ regulatory T (Treg) cells are pivotal for the maintenance of self-tolerance, and their adoptive transfer gives protection from autoimmune diseases and pathogenic alloresponses after solid organ or bone marrow transplantation in murine model systems. In vitro, human CD4(+)CD25+ Treg cells display phenotypic and functional characteristics similar to those of murine CD4(+)CD25+ Treg cells: namely, hyporesponsiveness to T-cell receptor (TCR) stimulation and suppression of CD25- T cells. Thus far, the detailed characterization and potential clinical application of human CD4(+)CD25+ Treg cells have been hampered by their paucity in peripheral blood and the lack of appropriate expansion protocols. Here we describe the up to 40 000-fold expansion of highly purified human CD4(+)CD25high T cells in vitro through the use of artificial antigen-presenting cells for repeated stimulation via CD3 and CD28 in the presence of high-dose interleukin 2 (IL-2). Expanded CD4(+)CD25high T cells were polyclonal, maintained their phenotype, exceeded the suppressive activity of freshly isolated CD4(+)CD25high T cells, and maintained expression of the lymph node homing receptors L-selectin (CD62L) and CCR7. The ability to rapidly expand human CD4(+)CD25high Treg cells on a large scale will not only facilitate their further exploration but also accelerate their potential clinical application in T cell-mediated diseases and transplantation medicine. SN - 0006-4971 UR - https://www.unboundmedicine.com/medline/citation/15090447/Large_scale_in_vitro_expansion_of_polyclonal_human_CD4_+_CD25high_regulatory_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/2004-01-0086 DB - PRIME DP - Unbound Medicine ER -