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Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium.
Biosens Bioelectron. 2004 Jun 15; 19(11):1497-504.BB

Abstract

An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Salmonella typhimurium. A protein G layer was fabricated by binding chemically to self-assembly monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) on gold (Au) surface. The formation of protein G layer on Au surface modified with 11-MUA and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The effect of detergent such as Tween-20 on binding efficiency of antibody and antigen was investigated by SPR. The binding efficiency of antigen to the antibody immobilized on Au surface was improved up to about 85% and 100% by using protein G and Tween-20, respectively. The surface morphology analyses of 11-MUA monolayer on Au substrate, protein G layer on 11-MUA monolayer and antibody layer immobilized on protein G layer were performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. typhimurium using protein G was developed with a detection range of 10(2) to 10(9)CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. typhimurium could be applied to construct other immnosensors or protein chips.

Authors+Show Affiliations

Oh BK
Department of Chemical and Biomolecular Engineering, Sogang University, 1 Shinsu-dong, Mapo-gu, Seoul 121-742, South Korea.
Kim YK
No affiliation info available
Park KW
No affiliation info available
Lee WH
No affiliation info available
Choi JW
No affiliation info available

MeSH

GoldMicroscopy, Atomic ForceNerve Tissue ProteinsSalmonella typhimuriumSurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15093222

Citation

Oh, Byung-Keun, et al. "Surface Plasmon Resonance Immunosensor for the Detection of Salmonella Typhimurium." Biosensors & Bioelectronics, vol. 19, no. 11, 2004, pp. 1497-504.
Oh BK, Kim YK, Park KW, et al. Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium. Biosens Bioelectron. 2004;19(11):1497-504.
Oh, B. K., Kim, Y. K., Park, K. W., Lee, W. H., & Choi, J. W. (2004). Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium. Biosensors & Bioelectronics, 19(11), 1497-504.
Oh BK, et al. Surface Plasmon Resonance Immunosensor for the Detection of Salmonella Typhimurium. Biosens Bioelectron. 2004 Jun 15;19(11):1497-504. PubMed PMID: 15093222.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium. AU - Oh,Byung-Keun, AU - Kim,Young-Kee, AU - Park,Kwang Won, AU - Lee,Won Hong, AU - Choi,Jeong-Woo, PY - 2003/09/17/received PY - 2003/12/03/accepted PY - 2004/4/20/pubmed PY - 2004/12/29/medline PY - 2004/4/20/entrez SP - 1497 EP - 504 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 19 IS - 11 N2 - An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Salmonella typhimurium. A protein G layer was fabricated by binding chemically to self-assembly monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) on gold (Au) surface. The formation of protein G layer on Au surface modified with 11-MUA and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The effect of detergent such as Tween-20 on binding efficiency of antibody and antigen was investigated by SPR. The binding efficiency of antigen to the antibody immobilized on Au surface was improved up to about 85% and 100% by using protein G and Tween-20, respectively. The surface morphology analyses of 11-MUA monolayer on Au substrate, protein G layer on 11-MUA monolayer and antibody layer immobilized on protein G layer were performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. typhimurium using protein G was developed with a detection range of 10(2) to 10(9)CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. typhimurium could be applied to construct other immnosensors or protein chips. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15093222/Surface_plasmon_resonance_immunosensor_for_the_detection_of_Salmonella_typhimurium_ DB - PRIME DP - Unbound Medicine ER -