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Micellar electrokinetic chromatography of enkephalin-related peptides with laser-induced fluorescence detection.
Electrophoresis. 2004 Apr; 25(7-8):1051-7.E

Abstract

Eight enkephalin-related peptides were derivatized using fluorescein isothiocyanate (FITC), and the derivatization products were separated and detected by capillary electrophoresis with laser-induced fluorescence detection. The optimum molar ratio of FITC and peptide for derivatization was found to be 40:1, and 5 mmol/L sodium borate buffer (pH 9.2) was selected as derivatization media in order to get the high efficiency. Enkephalin-related peptides were completely separated in the pH range of 10.51-10.60 in a running buffer consisting of 100 mmol/L sodium borate and 60 mmol/L sodium dodecyl sulfate (SDS). The detection limit for these eight enkephalins ranged from 0.18 to 2.25 nmol/L, and the linear response range was 1.0 x 10(-6) to 1.0 x 10(-9) mol/L with correlation coefficients between 0.9947-0.9988. A separation efficiency as high as 380000 theoretical plates could be obtained for these analytes.

Authors+Show Affiliations

Department of Chemistry, Fuzhou University, Fujian, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15095447

Citation

Huang, Ying, et al. "Micellar Electrokinetic Chromatography of Enkephalin-related Peptides With Laser-induced Fluorescence Detection." Electrophoresis, vol. 25, no. 7-8, 2004, pp. 1051-7.
Huang Y, Duan J, Chen Q, et al. Micellar electrokinetic chromatography of enkephalin-related peptides with laser-induced fluorescence detection. Electrophoresis. 2004;25(7-8):1051-7.
Huang, Y., Duan, J., Chen, Q., & Chen, G. (2004). Micellar electrokinetic chromatography of enkephalin-related peptides with laser-induced fluorescence detection. Electrophoresis, 25(7-8), 1051-7.
Huang Y, et al. Micellar Electrokinetic Chromatography of Enkephalin-related Peptides With Laser-induced Fluorescence Detection. Electrophoresis. 2004;25(7-8):1051-7. PubMed PMID: 15095447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic chromatography of enkephalin-related peptides with laser-induced fluorescence detection. AU - Huang,Ying, AU - Duan,Jianping, AU - Chen,Qiaoren, AU - Chen,Guonan, PY - 2004/4/20/pubmed PY - 2005/2/25/medline PY - 2004/4/20/entrez SP - 1051 EP - 7 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 7-8 N2 - Eight enkephalin-related peptides were derivatized using fluorescein isothiocyanate (FITC), and the derivatization products were separated and detected by capillary electrophoresis with laser-induced fluorescence detection. The optimum molar ratio of FITC and peptide for derivatization was found to be 40:1, and 5 mmol/L sodium borate buffer (pH 9.2) was selected as derivatization media in order to get the high efficiency. Enkephalin-related peptides were completely separated in the pH range of 10.51-10.60 in a running buffer consisting of 100 mmol/L sodium borate and 60 mmol/L sodium dodecyl sulfate (SDS). The detection limit for these eight enkephalins ranged from 0.18 to 2.25 nmol/L, and the linear response range was 1.0 x 10(-6) to 1.0 x 10(-9) mol/L with correlation coefficients between 0.9947-0.9988. A separation efficiency as high as 380000 theoretical plates could be obtained for these analytes. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15095447/Micellar_electrokinetic_chromatography_of_enkephalin_related_peptides_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/elps.200405810 DB - PRIME DP - Unbound Medicine ER -