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Chiral capillary electrophoretic resolution of baclofen, gabaergic ligand, using highly sulfated cyclodextrins.
Electrophoresis. 2004 Apr; 25(7-8):1111-9.E

Abstract

Using cyclodextrin-capillary zone electrophoresis (CD-CZE), baseline separation of baclofen, a potent GABA(B) agonist; was achieved. A method for the enantioresolution of this gamma-aminobutyric acid (GABA) and determination of enantiomeric purity was developed using CDs (highly sulfated-CD or highly S-CD) as chiral selectors and capillaries dynamically coated with polyethylene oxide (PEO). Operational parameters, such as the nature and concentration of the chiral selectors, buffer concentration, organic modifiers, and applied voltage, were investigated. The use of charged CDs provides a driving force in the opposite direction of the positively charged baclofen in the running buffer and enantiomeric resolution by inclusion of compounds in the CD cavity. Highly S-beta-CD was found to be the most effective complexing agent, allowing good enantiomeric resolution. The complete resolution was obtained using 25 mM phosphate buffer, pH 2.5, containing 3% w/v highly S-beta-CD at 25 degrees C with aN applied field of 0.40 kV/cm. The apparent association constants of the inclusion complexes were calculated. This optimized method was validated in terms of repeatability and limits of detection (0.13 microg x mL(-1)) and quantification. The migration order was determined.

Authors+Show Affiliations

Vaccher MP
Laboratoire de Chimie Analytique, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille, Lille, France.
Lipka E
No affiliation info available
Bonte JP
No affiliation info available
Vaccher C
No affiliation info available

MeSH

BaclofenBuffersCyclodextrinsElectrophoresis, CapillaryGABA AgonistsLigandsSensitivity and SpecificitySulfuric Acidsgamma-Aminobutyric Acid

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15095454

Citation

Vaccher, Marie-Pierre, et al. "Chiral Capillary Electrophoretic Resolution of Baclofen, Gabaergic Ligand, Using Highly Sulfated Cyclodextrins." Electrophoresis, vol. 25, no. 7-8, 2004, pp. 1111-9.
Vaccher MP, Lipka E, Bonte JP, et al. Chiral capillary electrophoretic resolution of baclofen, gabaergic ligand, using highly sulfated cyclodextrins. Electrophoresis. 2004;25(7-8):1111-9.
Vaccher, M. P., Lipka, E., Bonte, J. P., & Vaccher, C. (2004). Chiral capillary electrophoretic resolution of baclofen, gabaergic ligand, using highly sulfated cyclodextrins. Electrophoresis, 25(7-8), 1111-9.
Vaccher MP, et al. Chiral Capillary Electrophoretic Resolution of Baclofen, Gabaergic Ligand, Using Highly Sulfated Cyclodextrins. Electrophoresis. 2004;25(7-8):1111-9. PubMed PMID: 15095454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chiral capillary electrophoretic resolution of baclofen, gabaergic ligand, using highly sulfated cyclodextrins. AU - Vaccher,Marie-Pierre, AU - Lipka,Emmanuelle, AU - Bonte,Jean-Paul, AU - Vaccher,Claude, PY - 2004/4/20/pubmed PY - 2005/2/25/medline PY - 2004/4/20/entrez SP - 1111 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 7-8 N2 - Using cyclodextrin-capillary zone electrophoresis (CD-CZE), baseline separation of baclofen, a potent GABA(B) agonist; was achieved. A method for the enantioresolution of this gamma-aminobutyric acid (GABA) and determination of enantiomeric purity was developed using CDs (highly sulfated-CD or highly S-CD) as chiral selectors and capillaries dynamically coated with polyethylene oxide (PEO). Operational parameters, such as the nature and concentration of the chiral selectors, buffer concentration, organic modifiers, and applied voltage, were investigated. The use of charged CDs provides a driving force in the opposite direction of the positively charged baclofen in the running buffer and enantiomeric resolution by inclusion of compounds in the CD cavity. Highly S-beta-CD was found to be the most effective complexing agent, allowing good enantiomeric resolution. The complete resolution was obtained using 25 mM phosphate buffer, pH 2.5, containing 3% w/v highly S-beta-CD at 25 degrees C with aN applied field of 0.40 kV/cm. The apparent association constants of the inclusion complexes were calculated. This optimized method was validated in terms of repeatability and limits of detection (0.13 microg x mL(-1)) and quantification. The migration order was determined. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15095454/Chiral_capillary_electrophoretic_resolution_of_baclofen_gabaergic_ligand_using_highly_sulfated_cyclodextrins_ DB - PRIME DP - Unbound Medicine ER -