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Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin.
Trans R Soc Trop Med Hyg. 2004 Jun; 98(6):360-6.TR

Abstract

A nested PCR technique was performed to detect a specific 483 bp DNA fragment of Orientia tsutsugamushi, the aetiological agent of scrub typhus, in 53 blood samples from 36 patients with acute pyrexia of unknown origin in southern Thailand. The specific primers could amplify the specific DNA from all 10 prototype strains of O. tsutsugamushi and all nine seropositive patients and three seronegative patients, while no DNA amplification was obtained with DNAs from other rickettsiae or from healthy persons or from patients with murine typhus. The specific PCR product was detectable in the blood for as long as 22 days after the onset of disease in patients without specific treatment and 27 days after receiving a single dose of doxycycline. Thus, nested PCR may be more sensitive than the serological test for diagnosis of scrub typhus and prolonged persistence of O. tsutsugamushi DNA in patients' blood was demonstrated despite clinical recovery of the patients.

Authors+Show Affiliations

Department of Medical Science, National Institute of Health, Nonthaburi, Thailand.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

15099992

Citation

Saisongkorh, Watcharee, et al. "Evaluation of Nested PCR for the Diagnosis of Scrub Typhus Among Patients With Acute Pyrexia of Unknown Origin." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 98, no. 6, 2004, pp. 360-6.
Saisongkorh W, Chenchittikul M, Silpapojakul K. Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin. Trans R Soc Trop Med Hyg. 2004;98(6):360-6.
Saisongkorh, W., Chenchittikul, M., & Silpapojakul, K. (2004). Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin. Transactions of the Royal Society of Tropical Medicine and Hygiene, 98(6), 360-6.
Saisongkorh W, Chenchittikul M, Silpapojakul K. Evaluation of Nested PCR for the Diagnosis of Scrub Typhus Among Patients With Acute Pyrexia of Unknown Origin. Trans R Soc Trop Med Hyg. 2004;98(6):360-6. PubMed PMID: 15099992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin. AU - Saisongkorh,Watcharee, AU - Chenchittikul,Mongkol, AU - Silpapojakul,Khachornsakdi, PY - 2003/06/03/received PY - 2003/10/27/revised PY - 2003/10/29/accepted PY - 2004/4/22/pubmed PY - 2004/7/13/medline PY - 2004/4/22/entrez SP - 360 EP - 6 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans. R. Soc. Trop. Med. Hyg. VL - 98 IS - 6 N2 - A nested PCR technique was performed to detect a specific 483 bp DNA fragment of Orientia tsutsugamushi, the aetiological agent of scrub typhus, in 53 blood samples from 36 patients with acute pyrexia of unknown origin in southern Thailand. The specific primers could amplify the specific DNA from all 10 prototype strains of O. tsutsugamushi and all nine seropositive patients and three seronegative patients, while no DNA amplification was obtained with DNAs from other rickettsiae or from healthy persons or from patients with murine typhus. The specific PCR product was detectable in the blood for as long as 22 days after the onset of disease in patients without specific treatment and 27 days after receiving a single dose of doxycycline. Thus, nested PCR may be more sensitive than the serological test for diagnosis of scrub typhus and prolonged persistence of O. tsutsugamushi DNA in patients' blood was demonstrated despite clinical recovery of the patients. SN - 0035-9203 UR - https://www.unboundmedicine.com/medline/citation/15099992/Evaluation_of_nested_PCR_for_the_diagnosis_of_scrub_typhus_among_patients_with_acute_pyrexia_of_unknown_origin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035920304000331 DB - PRIME DP - Unbound Medicine ER -