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Induction and binding of Cd, Cu, and Zn to metallothionein in carp (Cyprinus carpio) using HPLC-ICP-TOFMS.
Toxicol Sci. 2004 Aug; 80(2):276-87.TS

Abstract

The binding of Cd, Cu, and Zn to metallothionein in carp was studied under control and acute Cd exposure scenarios. Carp were exposed to different Cd concentrations for 96 h. Total (Cu, Cd, Zn)-MT levels were determined by the cadmium thiomolybdate saturation assay. Total tissue and cytosolic Cd, Cu, and Zn concentrations were determined by ICP-MS. The cytosolic metal speciation was determined by high pressure liquid chromatography (size-exclusion [SE] in combination with anion exchange [AE]) directly coupled to an inductively coupled plasma time of flight mass spectrometer (ICP-TOFMS). This coupled technique allows the chromatographic separation and online determination of the metals associated to the protein fractions separated. Very strong differences in the tissue compartmentalization and cytosolic speciation of the metals were observed. For example, over 30% of cytosolic zinc was bound to MT in liver while this was only 2% in the kidneys although total cytosolic levels were considerably higher. Induction of metallothionein during cadmium exposure was also tissue specific, displaying different response patterns in gills, liver, and kidney. Cadmium accumulated much stronger in liver and kidney compared to the gills and the latter also showed much lower MT levels. The renal MT-induction was more sensitive to Cd exposure than the hepatic MT induction since a significant increase of Cd-MT and total MT levels occurred at lower tissue Cd concentrations in the kidney in comparison to the liver, except for the highest Cd exposure level where a drastic 10-fold increase in hepatic Cd-MT was observed. At this Cd exposure level also an apparent spill over of zinc to the high molecular weight fraction was observed in the kidneys.

Authors+Show Affiliations

Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. karen.vancampenhout@@ua.ac.beNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15103052

Citation

Van Campenhout, Karen, et al. "Induction and Binding of Cd, Cu, and Zn to Metallothionein in Carp (Cyprinus Carpio) Using HPLC-ICP-TOFMS." Toxicological Sciences : an Official Journal of the Society of Toxicology, vol. 80, no. 2, 2004, pp. 276-87.
Van Campenhout K, Infante HG, Adams F, et al. Induction and binding of Cd, Cu, and Zn to metallothionein in carp (Cyprinus carpio) using HPLC-ICP-TOFMS. Toxicol Sci. 2004;80(2):276-87.
Van Campenhout, K., Infante, H. G., Adams, F., & Blust, R. (2004). Induction and binding of Cd, Cu, and Zn to metallothionein in carp (Cyprinus carpio) using HPLC-ICP-TOFMS. Toxicological Sciences : an Official Journal of the Society of Toxicology, 80(2), 276-87.
Van Campenhout K, et al. Induction and Binding of Cd, Cu, and Zn to Metallothionein in Carp (Cyprinus Carpio) Using HPLC-ICP-TOFMS. Toxicol Sci. 2004;80(2):276-87. PubMed PMID: 15103052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction and binding of Cd, Cu, and Zn to metallothionein in carp (Cyprinus carpio) using HPLC-ICP-TOFMS. AU - Van Campenhout,Karen, AU - Infante,Heidi Goenaga, AU - Adams,Freddy, AU - Blust,Ronny, Y1 - 2004/04/21/ PY - 2004/4/23/pubmed PY - 2005/1/6/medline PY - 2004/4/23/entrez SP - 276 EP - 87 JF - Toxicological sciences : an official journal of the Society of Toxicology JO - Toxicol Sci VL - 80 IS - 2 N2 - The binding of Cd, Cu, and Zn to metallothionein in carp was studied under control and acute Cd exposure scenarios. Carp were exposed to different Cd concentrations for 96 h. Total (Cu, Cd, Zn)-MT levels were determined by the cadmium thiomolybdate saturation assay. Total tissue and cytosolic Cd, Cu, and Zn concentrations were determined by ICP-MS. The cytosolic metal speciation was determined by high pressure liquid chromatography (size-exclusion [SE] in combination with anion exchange [AE]) directly coupled to an inductively coupled plasma time of flight mass spectrometer (ICP-TOFMS). This coupled technique allows the chromatographic separation and online determination of the metals associated to the protein fractions separated. Very strong differences in the tissue compartmentalization and cytosolic speciation of the metals were observed. For example, over 30% of cytosolic zinc was bound to MT in liver while this was only 2% in the kidneys although total cytosolic levels were considerably higher. Induction of metallothionein during cadmium exposure was also tissue specific, displaying different response patterns in gills, liver, and kidney. Cadmium accumulated much stronger in liver and kidney compared to the gills and the latter also showed much lower MT levels. The renal MT-induction was more sensitive to Cd exposure than the hepatic MT induction since a significant increase of Cd-MT and total MT levels occurred at lower tissue Cd concentrations in the kidney in comparison to the liver, except for the highest Cd exposure level where a drastic 10-fold increase in hepatic Cd-MT was observed. At this Cd exposure level also an apparent spill over of zinc to the high molecular weight fraction was observed in the kidneys. SN - 1096-6080 UR - https://www.unboundmedicine.com/medline/citation/15103052/Induction_and_binding_of_Cd_Cu_and_Zn_to_metallothionein_in_carp__Cyprinus_carpio__using_HPLC_ICP_TOFMS_ L2 - https://academic.oup.com/toxsci/article-lookup/doi/10.1093/toxsci/kfh149 DB - PRIME DP - Unbound Medicine ER -