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The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer.
Biomed Chromatogr. 2004 Apr; 18(3):150-4.BC

Abstract

Capillary electrophoresis has been applied the analyses of many clinical drugs due to its rapid, high-resolution separation. In this study, electrokinetic chromatography involving the combination of SDS and dextran sulfate, which are synthetic polymers, was examined in order to obtain high resolution. Use of 2% dextran sulfate (10,000 molecular weight), 20 mm SDS running buffer containing boric acid solution (pH 9.2) and a silica capillary (inner diameter of 75 micro m, effective length of 50 cm, 57 cm overall length) afforded separation of 10 kinds of benzodiazepines. The detection limit was 0.2 micro g/mL; additionally, reproducibilities were de fi ned as the peak height and migration time. The average peak height was 5.92% (2.46-17.61), whereas the average migration time was 0.44% (0.18-0.76; n = 5). This separations system can be applied to the analysis and measurement of other pharmaceuticals as well.

Authors+Show Affiliations

Suzuki Y
School of Pharmaceutical Sciences, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan.
Arakawa H
No affiliation info available
Maeda M
No affiliation info available

MeSH

BenzodiazepinesChromatography, Micellar Electrokinetic CapillaryDextran SulfateElectrophoresis, CapillarySodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15103699

Citation

Suzuki, Yoshio, et al. "The Capillary Electrophoresis Separation of Benzodiazepine Drug Using Dextran Sulfate and SDS as Running Buffer." Biomedical Chromatography : BMC, vol. 18, no. 3, 2004, pp. 150-4.
Suzuki Y, Arakawa H, Maeda M. The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer. Biomed Chromatogr. 2004;18(3):150-4.
Suzuki, Y., Arakawa, H., & Maeda, M. (2004). The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer. Biomedical Chromatography : BMC, 18(3), 150-4.
Suzuki Y, Arakawa H, Maeda M. The Capillary Electrophoresis Separation of Benzodiazepine Drug Using Dextran Sulfate and SDS as Running Buffer. Biomed Chromatogr. 2004;18(3):150-4. PubMed PMID: 15103699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The capillary electrophoresis separation of benzodiazepine drug using dextran sulfate and SDS as running buffer. AU - Suzuki,Yoshio, AU - Arakawa,Hidetoshi, AU - Maeda,Masako, PY - 2004/4/23/pubmed PY - 2004/12/22/medline PY - 2004/4/23/entrez SP - 150 EP - 4 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 3 N2 - Capillary electrophoresis has been applied the analyses of many clinical drugs due to its rapid, high-resolution separation. In this study, electrokinetic chromatography involving the combination of SDS and dextran sulfate, which are synthetic polymers, was examined in order to obtain high resolution. Use of 2% dextran sulfate (10,000 molecular weight), 20 mm SDS running buffer containing boric acid solution (pH 9.2) and a silica capillary (inner diameter of 75 micro m, effective length of 50 cm, 57 cm overall length) afforded separation of 10 kinds of benzodiazepines. The detection limit was 0.2 micro g/mL; additionally, reproducibilities were de fi ned as the peak height and migration time. The average peak height was 5.92% (2.46-17.61), whereas the average migration time was 0.44% (0.18-0.76; n = 5). This separations system can be applied to the analysis and measurement of other pharmaceuticals as well. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15103699/The_capillary_electrophoresis_separation_of_benzodiazepine_drug_using_dextran_sulfate_and_SDS_as_running_buffer_ DB - PRIME DP - Unbound Medicine ER -