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Identification and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography.
Biomed Chromatogr. 2004 Apr; 18(3):167-72.BC

Abstract

The simultaneous determination of emodin, aloe-emodin and rhein, in Semen Cassiae, Qinghai Wild Dahuang tea, Ningxia Juemingzi tea, Lanzhou Juemingzi tea and Surong Juemingzi tea, has been investigated by micellar electrokinetic capillary chromatography for the fi rst time. With an electrolyte containing 15 mm borax, 30 mm SDS, 10% (v/v) ethanol, at pH 9.60 and 20 kV applied voltage, the three analytes were completely separated within 12 min. The effects of the concentration of borax, electrolyte pH, the concentrations of SDS and organic modifier and the applied voltage on electropheoretic behavior and separation were studied. The linear calibration range was 4-120 micro g/mL (r = 0.9921) for emodin, 10-200 micro g/mL (r = 0.9970) for aloe-emodin and 2-100 micro g/mL (r = 0.9971) for rhein, respectively. Under the optimum conditions, the relative standard deviation (RSD) values (n = 6) of the migration time and the peak area of each peak was 0.59-0.80% and 1.30-3.22%, respectively. The contents of the analytes in Rheum, Qinghai Wild Dahuang tea, Semen Cassiae and three other kinds of teas were easily determined with recoveries ranging from 95.3 to 104.4%.

Authors+Show Affiliations

Zheng W
Department of Chemistry, Lanzhou University, Lanzhou, 730000, People's Republic of China.
Wang S
No affiliation info available
Chen X
No affiliation info available
Hu Z
No affiliation info available

MeSH

AnthraquinonesBoratesCalibrationChromatography, Micellar Electrokinetic CapillaryElectrolytesEthanolHydrogen-Ion ConcentrationReproducibility of ResultsSensitivity and SpecificitySodium Dodecyl SulfateTea

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15103702

Citation

Zheng, Wenjie, et al. "Identification and Determination of Active Anthraquinones in Chinese Teas By Micellar Electrokinetic Capillary Chromatography." Biomedical Chromatography : BMC, vol. 18, no. 3, 2004, pp. 167-72.
Zheng W, Wang S, Chen X, et al. Identification and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography. Biomed Chromatogr. 2004;18(3):167-72.
Zheng, W., Wang, S., Chen, X., & Hu, Z. (2004). Identification and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography. Biomedical Chromatography : BMC, 18(3), 167-72.
Zheng W, et al. Identification and Determination of Active Anthraquinones in Chinese Teas By Micellar Electrokinetic Capillary Chromatography. Biomed Chromatogr. 2004;18(3):167-72. PubMed PMID: 15103702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and determination of active anthraquinones in Chinese teas by micellar electrokinetic capillary chromatography. AU - Zheng,Wenjie, AU - Wang,Shufang, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2004/4/23/pubmed PY - 2004/12/22/medline PY - 2004/4/23/entrez SP - 167 EP - 72 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 3 N2 - The simultaneous determination of emodin, aloe-emodin and rhein, in Semen Cassiae, Qinghai Wild Dahuang tea, Ningxia Juemingzi tea, Lanzhou Juemingzi tea and Surong Juemingzi tea, has been investigated by micellar electrokinetic capillary chromatography for the fi rst time. With an electrolyte containing 15 mm borax, 30 mm SDS, 10% (v/v) ethanol, at pH 9.60 and 20 kV applied voltage, the three analytes were completely separated within 12 min. The effects of the concentration of borax, electrolyte pH, the concentrations of SDS and organic modifier and the applied voltage on electropheoretic behavior and separation were studied. The linear calibration range was 4-120 micro g/mL (r = 0.9921) for emodin, 10-200 micro g/mL (r = 0.9970) for aloe-emodin and 2-100 micro g/mL (r = 0.9971) for rhein, respectively. Under the optimum conditions, the relative standard deviation (RSD) values (n = 6) of the migration time and the peak area of each peak was 0.59-0.80% and 1.30-3.22%, respectively. The contents of the analytes in Rheum, Qinghai Wild Dahuang tea, Semen Cassiae and three other kinds of teas were easily determined with recoveries ranging from 95.3 to 104.4%. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15103702/Identification_and_determination_of_active_anthraquinones_in_Chinese_teas_by_micellar_electrokinetic_capillary_chromatography_ DB - PRIME DP - Unbound Medicine ER -