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High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label.
Biomed Chromatogr. 2004 Apr; 18(3):202-4.BC

Abstract

This paper describes a highly sensitive HPLC method for the simultaneous determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in human hair samples. The amphetamines investigated were derivatized with the fluorescent reagent, DIB-Cl to yield highly fluorescent DIB-derivatives, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 nm and 430 nm, respectively. The separation was achieved on an ODS column with an isocratic mobile phase composed of acetonitrile-methanol-water (30:40:30, v/v/v). The limits of detection for the four compounds obtained by the proposed method ranged from 11 to 200 pg/mg. The method was successfully applied to the determination of MDMA and MDA in hair samples obtained from MDMA abuser.

Authors+Show Affiliations

Division of Analytical Research for Pharmacoinformatics, Department of Clinical Pharmacy, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15103707

Citation

Kaddoumi, Amal, et al. "High-performance Liquid Chromatography With Fluorescence Detection for the Simultaneous Determination of 3,4-methylenedioxymethamphetamine, Methamphetamine and Their Metabolites in Human Hair Using DIB-Cl as a Label." Biomedical Chromatography : BMC, vol. 18, no. 3, 2004, pp. 202-4.
Kaddoumi A, Kikura-Hanajiri R, Nakashima K. High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label. Biomed Chromatogr. 2004;18(3):202-4.
Kaddoumi, A., Kikura-Hanajiri, R., & Nakashima, K. (2004). High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label. Biomedical Chromatography : BMC, 18(3), 202-4.
Kaddoumi A, Kikura-Hanajiri R, Nakashima K. High-performance Liquid Chromatography With Fluorescence Detection for the Simultaneous Determination of 3,4-methylenedioxymethamphetamine, Methamphetamine and Their Metabolites in Human Hair Using DIB-Cl as a Label. Biomed Chromatogr. 2004;18(3):202-4. PubMed PMID: 15103707.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatography with fluorescence detection for the simultaneous determination of 3,4-methylenedioxymethamphetamine, methamphetamine and their metabolites in human hair using DIB-Cl as a label. AU - Kaddoumi,Amal, AU - Kikura-Hanajiri,Ruri, AU - Nakashima,Kenichiro, PY - 2004/4/23/pubmed PY - 2004/12/22/medline PY - 2004/4/23/entrez SP - 202 EP - 4 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 3 N2 - This paper describes a highly sensitive HPLC method for the simultaneous determination of 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), amphetamine (AP) and methamphetamine (MP) in human hair samples. The amphetamines investigated were derivatized with the fluorescent reagent, DIB-Cl to yield highly fluorescent DIB-derivatives, which were then analyzed by HPLC with fluorescence detection at excitation and emission wavelengths of 325 nm and 430 nm, respectively. The separation was achieved on an ODS column with an isocratic mobile phase composed of acetonitrile-methanol-water (30:40:30, v/v/v). The limits of detection for the four compounds obtained by the proposed method ranged from 11 to 200 pg/mg. The method was successfully applied to the determination of MDMA and MDA in hair samples obtained from MDMA abuser. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15103707/High_performance_liquid_chromatography_with_fluorescence_detection_for_the_simultaneous_determination_of_34_methylenedioxymethamphetamine_methamphetamine_and_their_metabolites_in_human_hair_using_DIB_Cl_as_a_label_ L2 - https://doi.org/10.1002/bmc.374 DB - PRIME DP - Unbound Medicine ER -