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Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone.
J Bone Miner Metab. 2004; 22(3):176-84.JB

Abstract

The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE (rhMEPE) after cloning its cDNA from the cDNA library of a nasal tumor tissue causing OHO. Using this antibody, we analyzed the distribution of MEPE in human bones by immunohistochemistry. In bone specimens from normal subjects, MEPE was predominantly expressed by osteocytes and not by osteoblasts. In bone specimens from patients with osteomalacia, however, MEPE was focally expressed by deeply located osteocytes. We also compared the MEPE positivity of osteocytes in mineralized bone and non-mineralized osteoid obtained from patients with osteomalacia and osteoporosis. Among osteomalacia patients, MEPE positivity was seen in 87.5 +/- 8.6% of the osteocytes from mineralized bone compared with 7.8 +/- 6.4% of those from osteoid. Among osteoporosis patients, MEPE positivity was found in 95.3 +/- 0.5% of the osteocytes from mineralized bone compared with 4.9 +/- 5.7% of those from osteoid. MEPE was mainly expressed by osteocytes embedded in the matrix of mineralized bone from patients with osteomalacia or osteoporosis. Our data provide the first histological evidence that MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone.

Authors+Show Affiliations

Department of Orthopaedics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15108058

Citation

Nampei, Akihide, et al. "Matrix Extracellular Phosphoglycoprotein (MEPE) Is Highly Expressed in Osteocytes in Human Bone." Journal of Bone and Mineral Metabolism, vol. 22, no. 3, 2004, pp. 176-84.
Nampei A, Hashimoto J, Hayashida K, et al. Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone. J Bone Miner Metab. 2004;22(3):176-84.
Nampei, A., Hashimoto, J., Hayashida, K., Tsuboi, H., Shi, K., Tsuji, I., Miyashita, H., Yamada, T., Matsukawa, N., Matsumoto, M., Morimoto, S., Ogihara, T., Ochi, T., & Yoshikawa, H. (2004). Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone. Journal of Bone and Mineral Metabolism, 22(3), 176-84.
Nampei A, et al. Matrix Extracellular Phosphoglycoprotein (MEPE) Is Highly Expressed in Osteocytes in Human Bone. J Bone Miner Metab. 2004;22(3):176-84. PubMed PMID: 15108058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone. AU - Nampei,Akihide, AU - Hashimoto,Jun, AU - Hayashida,Kenji, AU - Tsuboi,Hideki, AU - Shi,Kenrin, AU - Tsuji,Isamu, AU - Miyashita,Hideaki, AU - Yamada,Takao, AU - Matsukawa,Naomichi, AU - Matsumoto,Masayuki, AU - Morimoto,Shigeto, AU - Ogihara,Toshio, AU - Ochi,Takahiro, AU - Yoshikawa,Hideki, PY - 2003/05/23/received PY - 2003/09/12/accepted PY - 2004/4/27/pubmed PY - 2005/1/12/medline PY - 2004/4/27/entrez SP - 176 EP - 84 JF - Journal of bone and mineral metabolism JO - J. Bone Miner. Metab. VL - 22 IS - 3 N2 - The matrix extracellular phosphoglycoprotein (MEPE) gene is highly expressed in tumors that cause oncogenic hypophosphatemic osteomalacia (OHO). MEPE is also known as one of the bone-tooth matrix proteins and is associated with bone mineralization. We developed a rabbit polyclonal antibody directed against recombinant human MEPE (rhMEPE) after cloning its cDNA from the cDNA library of a nasal tumor tissue causing OHO. Using this antibody, we analyzed the distribution of MEPE in human bones by immunohistochemistry. In bone specimens from normal subjects, MEPE was predominantly expressed by osteocytes and not by osteoblasts. In bone specimens from patients with osteomalacia, however, MEPE was focally expressed by deeply located osteocytes. We also compared the MEPE positivity of osteocytes in mineralized bone and non-mineralized osteoid obtained from patients with osteomalacia and osteoporosis. Among osteomalacia patients, MEPE positivity was seen in 87.5 +/- 8.6% of the osteocytes from mineralized bone compared with 7.8 +/- 6.4% of those from osteoid. Among osteoporosis patients, MEPE positivity was found in 95.3 +/- 0.5% of the osteocytes from mineralized bone compared with 4.9 +/- 5.7% of those from osteoid. MEPE was mainly expressed by osteocytes embedded in the matrix of mineralized bone from patients with osteomalacia or osteoporosis. Our data provide the first histological evidence that MEPE is predominantly expressed by osteocytes in human bone, with significant expression by osteocytes within mineralized bone. SN - 0914-8779 UR - https://www.unboundmedicine.com/medline/citation/15108058/Matrix_extracellular_phosphoglycoprotein__MEPE__is_highly_expressed_in_osteocytes_in_human_bone_ L2 - https://dx.doi.org/10.1007/s00774-003-0468-9 DB - PRIME DP - Unbound Medicine ER -