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Association of vitamin D and estrogen receptor gene polymorphism with the effects of longterm hormone replacement therapy on bone mineral density.
J Bone Miner Metab. 2004; 22(3):241-7.JB

Abstract

We longitudinally studied whether vitamin D receptor (VDR) and estrogen receptor (ER) gene polymorphisms in Japanese women influenced the effect of longterm hormone replacement therapy (HRT) on bone mineral density (BMD) in the lumbar spine. The 81 subjects were aged 40 to 64 years (mean +/- SEM, 49.5 +/- 0.6 years), and had received sequential or continuous HRT regimens, including 0.625 mg of conjugated equine estrogen and 2.5 to 5 mg of medroxy-progesterone acetate, for at least 3 years. Genomic DNA was extracted from blood cells, and analyzed for restriction fragment length polymorphism, using the restriction endonucleases Taq I, Apa I, and Fok I for VDR, and Pvu II and Xba I for ER. At 1 year, subjects with a Taq I genotype of TT (i.e., site absent) showed a significantly greater increase in BMD with treatment (DeltaBMD) than subjects with the Tt genotype (2.6 +/- 0.5% vs -0.8 +/- 1.4%; P = 0.016). A small difference between genotypes remained at 2 years (3.8 +/- 0.6% vs 0.8 +/- 1.6%; P = 0.069), but no significant difference between genotypes was seen at 3 years. In multiple regression analyses, DeltaBMD at 1 year was significantly affected by VDR- Taq I, Apa I, and ER- Pvu II genotypes and by age at treatment initiation, although at 3 years or more, DeltaBMD was significantly affected only by age. These results indicate that Taq I VDR gene polymorphism predicted the effect on lumbar BMD for the first year of HRT in Japanese women, and that the differences in BMD versus the polymorphism disappeared if the treatment was continued for over 2 years.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951-8510, Japan. takumi@med.niigata-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15108066

Citation

Kurabayashi, Takumi, et al. "Association of Vitamin D and Estrogen Receptor Gene Polymorphism With the Effects of Longterm Hormone Replacement Therapy On Bone Mineral Density." Journal of Bone and Mineral Metabolism, vol. 22, no. 3, 2004, pp. 241-7.
Kurabayashi T, Matsushita H, Tomita M, et al. Association of vitamin D and estrogen receptor gene polymorphism with the effects of longterm hormone replacement therapy on bone mineral density. J Bone Miner Metab. 2004;22(3):241-7.
Kurabayashi, T., Matsushita, H., Tomita, M., Kato, N., Kikuchi, M., Nagata, H., Honda, A., Yahata, T., & Tanaka, K. (2004). Association of vitamin D and estrogen receptor gene polymorphism with the effects of longterm hormone replacement therapy on bone mineral density. Journal of Bone and Mineral Metabolism, 22(3), 241-7.
Kurabayashi T, et al. Association of Vitamin D and Estrogen Receptor Gene Polymorphism With the Effects of Longterm Hormone Replacement Therapy On Bone Mineral Density. J Bone Miner Metab. 2004;22(3):241-7. PubMed PMID: 15108066.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of vitamin D and estrogen receptor gene polymorphism with the effects of longterm hormone replacement therapy on bone mineral density. AU - Kurabayashi,Takumi, AU - Matsushita,Hiroshi, AU - Tomita,Masatoshi, AU - Kato,Nozomi, AU - Kikuchi,Mariko, AU - Nagata,Hiroshi, AU - Honda,Akira, AU - Yahata,Tetsuro, AU - Tanaka,Kenichi, PY - 2003/03/26/received PY - 2003/07/23/accepted PY - 2004/4/27/pubmed PY - 2005/1/12/medline PY - 2004/4/27/entrez SP - 241 EP - 7 JF - Journal of bone and mineral metabolism JO - J Bone Miner Metab VL - 22 IS - 3 N2 - We longitudinally studied whether vitamin D receptor (VDR) and estrogen receptor (ER) gene polymorphisms in Japanese women influenced the effect of longterm hormone replacement therapy (HRT) on bone mineral density (BMD) in the lumbar spine. The 81 subjects were aged 40 to 64 years (mean +/- SEM, 49.5 +/- 0.6 years), and had received sequential or continuous HRT regimens, including 0.625 mg of conjugated equine estrogen and 2.5 to 5 mg of medroxy-progesterone acetate, for at least 3 years. Genomic DNA was extracted from blood cells, and analyzed for restriction fragment length polymorphism, using the restriction endonucleases Taq I, Apa I, and Fok I for VDR, and Pvu II and Xba I for ER. At 1 year, subjects with a Taq I genotype of TT (i.e., site absent) showed a significantly greater increase in BMD with treatment (DeltaBMD) than subjects with the Tt genotype (2.6 +/- 0.5% vs -0.8 +/- 1.4%; P = 0.016). A small difference between genotypes remained at 2 years (3.8 +/- 0.6% vs 0.8 +/- 1.6%; P = 0.069), but no significant difference between genotypes was seen at 3 years. In multiple regression analyses, DeltaBMD at 1 year was significantly affected by VDR- Taq I, Apa I, and ER- Pvu II genotypes and by age at treatment initiation, although at 3 years or more, DeltaBMD was significantly affected only by age. These results indicate that Taq I VDR gene polymorphism predicted the effect on lumbar BMD for the first year of HRT in Japanese women, and that the differences in BMD versus the polymorphism disappeared if the treatment was continued for over 2 years. SN - 0914-8779 UR - https://www.unboundmedicine.com/medline/citation/15108066/Association_of_vitamin_D_and_estrogen_receptor_gene_polymorphism_with_the_effects_of_longterm_hormone_replacement_therapy_on_bone_mineral_density_ L2 - https://dx.doi.org/10.1007/s00774-003-0474-y DB - PRIME DP - Unbound Medicine ER -