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Coronaviridae and SARS-associated coronavirus strain HSR1.
Emerg Infect Dis. 2004 Mar; 10(3):413-8.EI

Abstract

During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 microg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.

Authors+Show Affiliations

P2-P3 Laboratories, DIBIT, San Raffaele Scientific Institute, Via Olgettina n. 58, 20132 Milan, Italy. vicenzi.elisa@hsr.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15109406

Citation

Vicenzi, Elisa, et al. "Coronaviridae and SARS-associated Coronavirus Strain HSR1." Emerging Infectious Diseases, vol. 10, no. 3, 2004, pp. 413-8.
Vicenzi E, Canducci F, Pinna D, et al. Coronaviridae and SARS-associated coronavirus strain HSR1. Emerg Infect Dis. 2004;10(3):413-8.
Vicenzi, E., Canducci, F., Pinna, D., Mancini, N., Carletti, S., Lazzarin, A., Bordignon, C., Poli, G., & Clementi, M. (2004). Coronaviridae and SARS-associated coronavirus strain HSR1. Emerging Infectious Diseases, 10(3), 413-8.
Vicenzi E, et al. Coronaviridae and SARS-associated Coronavirus Strain HSR1. Emerg Infect Dis. 2004;10(3):413-8. PubMed PMID: 15109406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coronaviridae and SARS-associated coronavirus strain HSR1. AU - Vicenzi,Elisa, AU - Canducci,Filippo, AU - Pinna,Debora, AU - Mancini,Nicasio, AU - Carletti,Silvia, AU - Lazzarin,Adriano, AU - Bordignon,Claudio, AU - Poli,Guido, AU - Clementi,Massimo, PY - 2004/4/28/pubmed PY - 2004/5/27/medline PY - 2004/4/28/entrez SP - 413 EP - 8 JF - Emerging infectious diseases JO - Emerg Infect Dis VL - 10 IS - 3 N2 - During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 microg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features. SN - 1080-6040 UR - https://www.unboundmedicine.com/medline/citation/15109406/Coronaviridae_and_SARS_associated_coronavirus_strain_HSR1_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/15109406/ DB - PRIME DP - Unbound Medicine ER -