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Characterization of Epstein-Barr virus infection in a human signet ring cell gastric carcinoma cell line, HSC-39.
Microbes Infect 2004; 6(5):429-39MI

Abstract

In order to study the mechanism of Epstein-Barr virus (EBV) infection in gastric carcinoma cells, we characterized the EBV infection in signet ring cell line HSC-39, derived from a human gastric carcinoma. HSC-39 cells were highly susceptible to cell-free EBV infection by Akata and P3HR-1 EBV strains. EBV nuclear antigen (EBNA) and EBV-encoded small RNA (EBER) were detected in the infected cells. Akata and P3HR-1 EBV-infected cell clones were isolated by a limiting dilution technique. The Akata and P3HR-1 EBV-infected clones differed from each other in morphology and growth patterns. Akata EBV-infected clones had lower growth rates than did P3HR-1 EBV-infected clones in both liquid and soft agar mediums. Both the infected HSC-39 cells and the clones expressed EBNA1 and EBER, but did not express EBNA2, latent membrane protein (LMP) 1 and LMP2A. The Q promoter (p), but not the Cp/Wp for EBNA transcription, was active in the infected HSC-39 cells and all clones. No lytic infection was observed in either infected parental cells or any clones. Uninfected HSC-39 cells did not express a principal EBV receptor CD21; however, Akata but not P3HR-1 EBV-infected clones expressed low levels of CD21 mRNA. These results demonstrate that the cellular phenotypes of HSC-39 cells are altered by EBV infection in strain-specific manner. We propose the HSC-39 cell line as a model target for the study of the mechanism and significance of EBV infection in gastric carcinoma.

Authors+Show Affiliations

Division of Biosignaling, Department of Biomedical Sciences, School of Life Science, Faculty of Medicine, Tottori University, Yonago 683-8503, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15109957

Citation

Luo, Bing, et al. "Characterization of Epstein-Barr Virus Infection in a Human Signet Ring Cell Gastric Carcinoma Cell Line, HSC-39." Microbes and Infection, vol. 6, no. 5, 2004, pp. 429-39.
Luo B, Murakami M, Fukuda M, et al. Characterization of Epstein-Barr virus infection in a human signet ring cell gastric carcinoma cell line, HSC-39. Microbes Infect. 2004;6(5):429-39.
Luo, B., Murakami, M., Fukuda, M., Fujioka, A., Yanagihara, K., & Sairenji, T. (2004). Characterization of Epstein-Barr virus infection in a human signet ring cell gastric carcinoma cell line, HSC-39. Microbes and Infection, 6(5), pp. 429-39.
Luo B, et al. Characterization of Epstein-Barr Virus Infection in a Human Signet Ring Cell Gastric Carcinoma Cell Line, HSC-39. Microbes Infect. 2004;6(5):429-39. PubMed PMID: 15109957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Epstein-Barr virus infection in a human signet ring cell gastric carcinoma cell line, HSC-39. AU - Luo,Bing, AU - Murakami,Masanao, AU - Fukuda,Makoto, AU - Fujioka,Aki, AU - Yanagihara,Kazuyoshi, AU - Sairenji,Takeshi, PY - 2003/09/16/received PY - 2003/12/22/accepted PY - 2004/4/28/pubmed PY - 2004/10/29/medline PY - 2004/4/28/entrez SP - 429 EP - 39 JF - Microbes and infection JO - Microbes Infect. VL - 6 IS - 5 N2 - In order to study the mechanism of Epstein-Barr virus (EBV) infection in gastric carcinoma cells, we characterized the EBV infection in signet ring cell line HSC-39, derived from a human gastric carcinoma. HSC-39 cells were highly susceptible to cell-free EBV infection by Akata and P3HR-1 EBV strains. EBV nuclear antigen (EBNA) and EBV-encoded small RNA (EBER) were detected in the infected cells. Akata and P3HR-1 EBV-infected cell clones were isolated by a limiting dilution technique. The Akata and P3HR-1 EBV-infected clones differed from each other in morphology and growth patterns. Akata EBV-infected clones had lower growth rates than did P3HR-1 EBV-infected clones in both liquid and soft agar mediums. Both the infected HSC-39 cells and the clones expressed EBNA1 and EBER, but did not express EBNA2, latent membrane protein (LMP) 1 and LMP2A. The Q promoter (p), but not the Cp/Wp for EBNA transcription, was active in the infected HSC-39 cells and all clones. No lytic infection was observed in either infected parental cells or any clones. Uninfected HSC-39 cells did not express a principal EBV receptor CD21; however, Akata but not P3HR-1 EBV-infected clones expressed low levels of CD21 mRNA. These results demonstrate that the cellular phenotypes of HSC-39 cells are altered by EBV infection in strain-specific manner. We propose the HSC-39 cell line as a model target for the study of the mechanism and significance of EBV infection in gastric carcinoma. SN - 1286-4579 UR - https://www.unboundmedicine.com/medline/citation/15109957/Characterization_of_Epstein_Barr_virus_infection_in_a_human_signet_ring_cell_gastric_carcinoma_cell_line_HSC_39_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1286457904000668 DB - PRIME DP - Unbound Medicine ER -