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BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line.
Biochem Biophys Res Commun. 2004 May 21; 318(1):113-8.BB

Abstract

Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly in the dorso-medial region of the substantia nigra, which contains most of the surviving dopaminergic neurons, suggesting that alpha-SN expression might have a neuroprotective function in glial cells. To investigate this hypothesis, we established alpha-SN transfected C6 glioma cell line clones and evaluated the expression of neurotrophins using semi-quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Brain-derived neurotrophic factor (BDNF) was induced by overexpression of wild-type alpha-SN but not by that of A30P and A53T. These data suggest that the pathogenic alpha-SN mutations, A30P or A53T, are linked to the loss of BDNF production in glial cells.

Authors+Show Affiliations

Department of Neurology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyoku, Kyoto 606-8507, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15110760

Citation

Kohno, Ryuichi, et al. "BDNF Is Induced By Wild-type Alpha-synuclein but Not By the Two Mutants, A30P or A53T, in Glioma Cell Line." Biochemical and Biophysical Research Communications, vol. 318, no. 1, 2004, pp. 113-8.
Kohno R, Sawada H, Kawamoto Y, et al. BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line. Biochem Biophys Res Commun. 2004;318(1):113-8.
Kohno, R., Sawada, H., Kawamoto, Y., Uemura, K., Shibasaki, H., & Shimohama, S. (2004). BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line. Biochemical and Biophysical Research Communications, 318(1), 113-8.
Kohno R, et al. BDNF Is Induced By Wild-type Alpha-synuclein but Not By the Two Mutants, A30P or A53T, in Glioma Cell Line. Biochem Biophys Res Commun. 2004 May 21;318(1):113-8. PubMed PMID: 15110760.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line. AU - Kohno,Ryuichi, AU - Sawada,Hideyuki, AU - Kawamoto,Yasuhiro, AU - Uemura,Kengo, AU - Shibasaki,Hiroshi, AU - Shimohama,Shun, PY - 2004/04/01/received PY - 2004/4/28/pubmed PY - 2004/6/21/medline PY - 2004/4/28/entrez SP - 113 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 318 IS - 1 N2 - Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly in the dorso-medial region of the substantia nigra, which contains most of the surviving dopaminergic neurons, suggesting that alpha-SN expression might have a neuroprotective function in glial cells. To investigate this hypothesis, we established alpha-SN transfected C6 glioma cell line clones and evaluated the expression of neurotrophins using semi-quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Brain-derived neurotrophic factor (BDNF) was induced by overexpression of wild-type alpha-SN but not by that of A30P and A53T. These data suggest that the pathogenic alpha-SN mutations, A30P or A53T, are linked to the loss of BDNF production in glial cells. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/15110760/BDNF_is_induced_by_wild_type_alpha_synuclein_but_not_by_the_two_mutants_A30P_or_A53T_in_glioma_cell_line_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006291X04007193 DB - PRIME DP - Unbound Medicine ER -