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Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus.
Eur J Clin Microbiol Infect Dis. 2004 May; 23(5):389-92.EJ

Abstract

The emergence of heterogeneous populations of methicillin-resistant Staphylococcus aureus (MRSA) causes major problems in routine screening for MRSA. In heterogeneous MRSA populations, a proportion of bacterial cells show low-level resistance to oxacillin, with minimal inhibitory concentrations (MICs) of oxacillin ranging between 1 and 100 mg/l, while in homogeneous MRSA populations, the MIC of oxacillin for all cells is >100 mg/l. Routine oxacillin disk diffusion tests often fail to detect heterogeneous MRSA populations. In the present study, a recently proposed disk diffusion method that employs a cephamycin antibiotic (cefoxitin 30 microg; BD Sensi-disc, Becton Dickinson, Germany) was evaluated using 155 clinical isolates of S. aureus (73 mecA positive and 82 mecA negative). The results were compared with those of other MRSA screening techniques: a disk diffusion test with oxacillin 1 microg and cefoxitin 30 microg (BD Sensi-disc; Becton Dickinson), an MRSA latex agglutination test (Denka Seiken, Japan), and an oxacillin screen agar test (6 microg/ml; Becton Dickinson). Detection of the mecA gene by polymerase chain reaction was considered the gold standard. The performances of the different methods were determined and compared. The results showed that the cefoxitin disk diffusion test is preferable to the oxacillin disk diffusion method for routine screening to detect MRSA.

Authors+Show Affiliations

Laboratory Department of Microbiology, AZ St Jan AV, Ruddershove 10, 8000 Brugge, Belgium. barbara.cauwelier@pandora.beNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

15112072

Citation

Cauwelier, B, et al. "Evaluation of a Disk Diffusion Method With Cefoxitin (30 Microg) for Detection of Methicillin-resistant Staphylococcus Aureus." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 23, no. 5, 2004, pp. 389-92.
Cauwelier B, Gordts B, Descheemaecker P, et al. Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 2004;23(5):389-92.
Cauwelier, B., Gordts, B., Descheemaecker, P., & Van Landuyt, H. (2004). Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 23(5), 389-92.
Cauwelier B, et al. Evaluation of a Disk Diffusion Method With Cefoxitin (30 Microg) for Detection of Methicillin-resistant Staphylococcus Aureus. Eur J Clin Microbiol Infect Dis. 2004;23(5):389-92. PubMed PMID: 15112072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus. AU - Cauwelier,B, AU - Gordts,B, AU - Descheemaecker,P, AU - Van Landuyt,H, Y1 - 2004/04/27/ PY - 2004/4/28/pubmed PY - 2004/7/16/medline PY - 2004/4/28/entrez SP - 389 EP - 92 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 23 IS - 5 N2 - The emergence of heterogeneous populations of methicillin-resistant Staphylococcus aureus (MRSA) causes major problems in routine screening for MRSA. In heterogeneous MRSA populations, a proportion of bacterial cells show low-level resistance to oxacillin, with minimal inhibitory concentrations (MICs) of oxacillin ranging between 1 and 100 mg/l, while in homogeneous MRSA populations, the MIC of oxacillin for all cells is >100 mg/l. Routine oxacillin disk diffusion tests often fail to detect heterogeneous MRSA populations. In the present study, a recently proposed disk diffusion method that employs a cephamycin antibiotic (cefoxitin 30 microg; BD Sensi-disc, Becton Dickinson, Germany) was evaluated using 155 clinical isolates of S. aureus (73 mecA positive and 82 mecA negative). The results were compared with those of other MRSA screening techniques: a disk diffusion test with oxacillin 1 microg and cefoxitin 30 microg (BD Sensi-disc; Becton Dickinson), an MRSA latex agglutination test (Denka Seiken, Japan), and an oxacillin screen agar test (6 microg/ml; Becton Dickinson). Detection of the mecA gene by polymerase chain reaction was considered the gold standard. The performances of the different methods were determined and compared. The results showed that the cefoxitin disk diffusion test is preferable to the oxacillin disk diffusion method for routine screening to detect MRSA. SN - 0934-9723 UR - https://www.unboundmedicine.com/medline/citation/15112072/Evaluation_of_a_disk_diffusion_method_with_cefoxitin__30_microg__for_detection_of_methicillin_resistant_Staphylococcus_aureus_ L2 - https://dx.doi.org/10.1007/s10096-004-1130-8 DB - PRIME DP - Unbound Medicine ER -