Tags

Type your tag names separated by a space and hit enter

Detection of K-ras point mutation and telomerase activity during endoscopic retrograde cholangiopancreatography in diagnosis of pancreatic cancer.
World J Gastroenterol. 2004 May 01; 10(9):1337-40.WJ

Abstract

AIM

To study the value of monitoring K-ras point mutation at codon 12 and telomerase activity in exfoliated cells obtained from pancreatic duct brushings during endoscopic retrograde cholangiopancreatography (ERCP) in the diagnosis of pancreatic cancer.

METHODS

Exfoliated cells obtained from pancreatic duct brushings during ERCP were examined in 27 patients: 23 with pancreatic cancers, 4 with chronic pancreatitis. K-ras point mutation was detected with the polymerase chain reaction and restriction fragment-length polymorphism (PCR-RFLP). Telomerase activity was detected by PCR and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA).

RESULTS

The telomerase activities in 27 patients were measured in 21 exfoliated cell samples obtained from pancreatic duct brushings. D450 value of telomerase activities in pancreatic cancer and chronic pancreatitis were 0.446+/-0.27 and 0.041+/-0.0111, respectively. Seventy-seven point eight percent (14/18) of patients with pancreatic cancer and none of the patients with chronic pancreatitis showed telomerase activity in cells collected from pancreatic duct brushings when cutoff value of telomerase activity was set at 2.0. The K-ras gene mutation rate (72.2%) in pancreatic cancer was higher than that in chronic pancreatitis (33.3%) (P<0.05). In considering of both telomerase activities and K-ras point mutation, the total positive rate was 83.3%(15/18), and the specificity was 100%.

CONCLUSION

Changes of telomerase activities and K-ras point mutation at codon 12 may be an early event of malignant progression in pancreatic cancer. Detection of telomerase activity and K-ras point mutation at codon 12 may be complementary to each other, and is useful in diagnosis of pancreatic cancer.

Authors+Show Affiliations

Department of Gastroenterology, Affiliated Hospital of Nantong Medical College, Nantong 226001, Jiangsu Province, China. zhouguoxiong@pub.nt.jsinfo.netNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15112354

Citation

Zhou, Guo-Xiong, et al. "Detection of K-ras Point Mutation and Telomerase Activity During Endoscopic Retrograde Cholangiopancreatography in Diagnosis of Pancreatic Cancer." World Journal of Gastroenterology, vol. 10, no. 9, 2004, pp. 1337-40.
Zhou GX, Huang JF, Li ZS, et al. Detection of K-ras point mutation and telomerase activity during endoscopic retrograde cholangiopancreatography in diagnosis of pancreatic cancer. World J Gastroenterol. 2004;10(9):1337-40.
Zhou, G. X., Huang, J. F., Li, Z. S., Xu, G. M., Liu, F., & Zhang, H. (2004). Detection of K-ras point mutation and telomerase activity during endoscopic retrograde cholangiopancreatography in diagnosis of pancreatic cancer. World Journal of Gastroenterology, 10(9), 1337-40.
Zhou GX, et al. Detection of K-ras Point Mutation and Telomerase Activity During Endoscopic Retrograde Cholangiopancreatography in Diagnosis of Pancreatic Cancer. World J Gastroenterol. 2004 May 1;10(9):1337-40. PubMed PMID: 15112354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of K-ras point mutation and telomerase activity during endoscopic retrograde cholangiopancreatography in diagnosis of pancreatic cancer. AU - Zhou,Guo-Xiong, AU - Huang,Jie-Fei, AU - Li,Zhao-Shen, AU - Xu,Guo-Ming, AU - Liu,Feng, AU - Zhang,Hong, PY - 2004/4/28/pubmed PY - 2004/10/8/medline PY - 2004/4/28/entrez SP - 1337 EP - 40 JF - World journal of gastroenterology JO - World J. Gastroenterol. VL - 10 IS - 9 N2 - AIM: To study the value of monitoring K-ras point mutation at codon 12 and telomerase activity in exfoliated cells obtained from pancreatic duct brushings during endoscopic retrograde cholangiopancreatography (ERCP) in the diagnosis of pancreatic cancer. METHODS: Exfoliated cells obtained from pancreatic duct brushings during ERCP were examined in 27 patients: 23 with pancreatic cancers, 4 with chronic pancreatitis. K-ras point mutation was detected with the polymerase chain reaction and restriction fragment-length polymorphism (PCR-RFLP). Telomerase activity was detected by PCR and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA). RESULTS: The telomerase activities in 27 patients were measured in 21 exfoliated cell samples obtained from pancreatic duct brushings. D450 value of telomerase activities in pancreatic cancer and chronic pancreatitis were 0.446+/-0.27 and 0.041+/-0.0111, respectively. Seventy-seven point eight percent (14/18) of patients with pancreatic cancer and none of the patients with chronic pancreatitis showed telomerase activity in cells collected from pancreatic duct brushings when cutoff value of telomerase activity was set at 2.0. The K-ras gene mutation rate (72.2%) in pancreatic cancer was higher than that in chronic pancreatitis (33.3%) (P<0.05). In considering of both telomerase activities and K-ras point mutation, the total positive rate was 83.3%(15/18), and the specificity was 100%. CONCLUSION: Changes of telomerase activities and K-ras point mutation at codon 12 may be an early event of malignant progression in pancreatic cancer. Detection of telomerase activity and K-ras point mutation at codon 12 may be complementary to each other, and is useful in diagnosis of pancreatic cancer. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/15112354/Detection_of_K_ras_point_mutation_and_telomerase_activity_during_endoscopic_retrograde_cholangiopancreatography_in_diagnosis_of_pancreatic_cancer_ L2 - http://www.wjgnet.com/1007-9327/full/v10/i9/1337.htm DB - PRIME DP - Unbound Medicine ER -