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Sequential fractionation of grape seeds into oils, polyphenols, and procyanidins via a single system employing CO2-based fluids.
J Agric Food Chem. 2004 May 05; 52(9):2440-4.JA

Abstract

Pure supercritical CO(2) was used to remove >95% of the oil from the grape seeds. Subcritical CO(2) modified with methanol was used for the extraction of monomeric polyphenols, whereas pure methanol was used for the extraction of polyphenolic dimers/trimers and procyanidins from grape seed. At optimum conditions, 40% methanol-modified CO(2) removed >79% of catechin and epicatechin from the grape seed. This extract was light yellow in color, and no higher molecular weight procyanidins were detected. Extraction of the same sample after removal of the oils and polyphenols, but now under enhanced solvent extraction conditions using methanol as a solvent, provided a dark red solution shown via electrospray ionization HPLC-MS to contain a relatively high concentration of procyanidins. The uniqueness of the study is attested to by the use of CO(2)-based fluids and the employment of a single instrumental extraction system.

Authors+Show Affiliations

Department of Chemistry, Virginia Tech, Blacksburg, Virginia 24061-0212, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15113138

Citation

Ashraf-Khorassani, Mehdi, and Larry Thomas Taylor. "Sequential Fractionation of Grape Seeds Into Oils, Polyphenols, and Procyanidins Via a Single System Employing CO2-based Fluids." Journal of Agricultural and Food Chemistry, vol. 52, no. 9, 2004, pp. 2440-4.
Ashraf-Khorassani M, Taylor LT. Sequential fractionation of grape seeds into oils, polyphenols, and procyanidins via a single system employing CO2-based fluids. J Agric Food Chem. 2004;52(9):2440-4.
Ashraf-Khorassani, M., & Taylor, L. T. (2004). Sequential fractionation of grape seeds into oils, polyphenols, and procyanidins via a single system employing CO2-based fluids. Journal of Agricultural and Food Chemistry, 52(9), 2440-4.
Ashraf-Khorassani M, Taylor LT. Sequential Fractionation of Grape Seeds Into Oils, Polyphenols, and Procyanidins Via a Single System Employing CO2-based Fluids. J Agric Food Chem. 2004 May 5;52(9):2440-4. PubMed PMID: 15113138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequential fractionation of grape seeds into oils, polyphenols, and procyanidins via a single system employing CO2-based fluids. AU - Ashraf-Khorassani,Mehdi, AU - Taylor,Larry Thomas, PY - 2004/4/29/pubmed PY - 2004/6/29/medline PY - 2004/4/29/entrez SP - 2440 EP - 4 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 9 N2 - Pure supercritical CO(2) was used to remove >95% of the oil from the grape seeds. Subcritical CO(2) modified with methanol was used for the extraction of monomeric polyphenols, whereas pure methanol was used for the extraction of polyphenolic dimers/trimers and procyanidins from grape seed. At optimum conditions, 40% methanol-modified CO(2) removed >79% of catechin and epicatechin from the grape seed. This extract was light yellow in color, and no higher molecular weight procyanidins were detected. Extraction of the same sample after removal of the oils and polyphenols, but now under enhanced solvent extraction conditions using methanol as a solvent, provided a dark red solution shown via electrospray ionization HPLC-MS to contain a relatively high concentration of procyanidins. The uniqueness of the study is attested to by the use of CO(2)-based fluids and the employment of a single instrumental extraction system. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15113138/Sequential_fractionation_of_grape_seeds_into_oils_polyphenols_and_procyanidins_via_a_single_system_employing_CO2_based_fluids_ DB - PRIME DP - Unbound Medicine ER -