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Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal caco-2 cell monolayers.
J Agric Food Chem. 2004 May 05; 52(9):2518-26.JA

Abstract

Both chlorogenic and caffeic acids exhibited nonsaturable transport in Caco-2 cells, whereas caffeic acid also showed proton-coupled polarized absorption. Thus, the absorption efficiency of caffeic acid was greater than that of chlorogenic acid. Polarized transport of caffeic acid was inhibited by substrates of MCT such as benzoic and acetic acids. Almost all of the apically loaded chlorogenic and caffeic acid was retained on the apical side, and the transepithelial flux was inversely correlated with the paracellular permeability of Caco-2 cells. These results indicate that transport was mainly via paracellular diffusion, although caffeic acid was absorbed to a lesser extent by the monocarboxylic acid transporter (MCT). Furthermore, m-coumaric acid and 3-(m-hydroxyphenyl)propionic acid, the main metabolites of chlorogenic and caffeic acid by colonic microflora, competitively inhibited the transport of fluorescein, a known substrate of MCT. This suggests that their absorption could also be mediated by MCT. These findings have exemplified the physiological importance of MCT-mediated absorption in both phenolic acids per se and their colonic metabolites.

Authors+Show Affiliations

Applied Bioresearch Center, Research and Development Department, Kirin Brewery Company, Ltd., 3 Miyaharacho, Takasaki-shi, Gunma 370-1295, Japan. konishiy@kirin.co.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15113150

Citation

Konishi, Yutaka, and Shoko Kobayashi. "Transepithelial Transport of Chlorogenic Acid, Caffeic Acid, and Their Colonic Metabolites in Intestinal Caco-2 Cell Monolayers." Journal of Agricultural and Food Chemistry, vol. 52, no. 9, 2004, pp. 2518-26.
Konishi Y, Kobayashi S. Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal caco-2 cell monolayers. J Agric Food Chem. 2004;52(9):2518-26.
Konishi, Y., & Kobayashi, S. (2004). Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal caco-2 cell monolayers. Journal of Agricultural and Food Chemistry, 52(9), 2518-26.
Konishi Y, Kobayashi S. Transepithelial Transport of Chlorogenic Acid, Caffeic Acid, and Their Colonic Metabolites in Intestinal Caco-2 Cell Monolayers. J Agric Food Chem. 2004 May 5;52(9):2518-26. PubMed PMID: 15113150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transepithelial transport of chlorogenic acid, caffeic acid, and their colonic metabolites in intestinal caco-2 cell monolayers. AU - Konishi,Yutaka, AU - Kobayashi,Shoko, PY - 2004/4/29/pubmed PY - 2004/6/29/medline PY - 2004/4/29/entrez SP - 2518 EP - 26 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 9 N2 - Both chlorogenic and caffeic acids exhibited nonsaturable transport in Caco-2 cells, whereas caffeic acid also showed proton-coupled polarized absorption. Thus, the absorption efficiency of caffeic acid was greater than that of chlorogenic acid. Polarized transport of caffeic acid was inhibited by substrates of MCT such as benzoic and acetic acids. Almost all of the apically loaded chlorogenic and caffeic acid was retained on the apical side, and the transepithelial flux was inversely correlated with the paracellular permeability of Caco-2 cells. These results indicate that transport was mainly via paracellular diffusion, although caffeic acid was absorbed to a lesser extent by the monocarboxylic acid transporter (MCT). Furthermore, m-coumaric acid and 3-(m-hydroxyphenyl)propionic acid, the main metabolites of chlorogenic and caffeic acid by colonic microflora, competitively inhibited the transport of fluorescein, a known substrate of MCT. This suggests that their absorption could also be mediated by MCT. These findings have exemplified the physiological importance of MCT-mediated absorption in both phenolic acids per se and their colonic metabolites. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15113150/Transepithelial_transport_of_chlorogenic_acid_caffeic_acid_and_their_colonic_metabolites_in_intestinal_caco_2_cell_monolayers_ L2 - https://doi.org/10.1021/jf035407c DB - PRIME DP - Unbound Medicine ER -