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A Kunitz-type inhibitor of coleopteran proteases, isolated from Adenanthera pavonina L. seeds and its effect on Callosobruchus maculatus.
J Agric Food Chem. 2004 May 05; 52(9):2533-40.JA

Abstract

The cowpea weevil Callosobruchus maculatus is one of the major pests of Vigna unguiculata cowpea. Digestion in the cowpea weevil is facilitated by high levels of cysteine and aspartic acid proteinases. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (ApTI) isolated from Adenanthera pavonina seeds showed activity against papain. The inhibition of papain by ApTI was of the noncompetitive type, with a K(i) of 1 microM. ApTI was highly effective against digestive proteinases from C. maculatus, Acanthoscelides obtectus (bean weevil), and Zabrotes subfasciatus (Mexican bean weevil) and was moderately active against midgut proteinases from the boll weevil Anthonomus grandis and the mealworm Tenebrio molitor. In C. maculates fed an artificial diet containing 0.25% and 0.5% ApTI (w/w), the latter concentration caused 50% mortality and reduced larval weight gain by approximately 40%. The action of ApTI on C. maculatus larvae may involve the inhibition of ApTI-sensitive cysteine proteinases and binding to chitin components of the peritrophic membrane (or equivalent structures) in the weevil midgut.

Authors+Show Affiliations

Macedo ML
Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Ciências Naturais, Universidade Federal de Mato Grosso do Sul, CP 210, CEP 79603-011,Três Lagoas, MS, Brazil. bioplant@terra.com.br
de Sá CM
No affiliation info available
Freire MD
No affiliation info available
Parra JR
No affiliation info available

MeSH

AnimalsChitinColeopteraCysteine Proteinase InhibitorsFabaceaeLarvaPapainPeptidesPlant ProteinsSeeds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15113152

Citation

Macedo, Maria Lígia Rodrigues, et al. "A Kunitz-type Inhibitor of Coleopteran Proteases, Isolated From Adenanthera Pavonina L. Seeds and Its Effect On Callosobruchus Maculatus." Journal of Agricultural and Food Chemistry, vol. 52, no. 9, 2004, pp. 2533-40.
Macedo ML, de Sá CM, Freire MD, et al. A Kunitz-type inhibitor of coleopteran proteases, isolated from Adenanthera pavonina L. seeds and its effect on Callosobruchus maculatus. J Agric Food Chem. 2004;52(9):2533-40.
Macedo, M. L., de Sá, C. M., Freire, M. D., & Parra, J. R. (2004). A Kunitz-type inhibitor of coleopteran proteases, isolated from Adenanthera pavonina L. seeds and its effect on Callosobruchus maculatus. Journal of Agricultural and Food Chemistry, 52(9), 2533-40.
Macedo ML, et al. A Kunitz-type Inhibitor of Coleopteran Proteases, Isolated From Adenanthera Pavonina L. Seeds and Its Effect On Callosobruchus Maculatus. J Agric Food Chem. 2004 May 5;52(9):2533-40. PubMed PMID: 15113152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Kunitz-type inhibitor of coleopteran proteases, isolated from Adenanthera pavonina L. seeds and its effect on Callosobruchus maculatus. AU - Macedo,Maria Lígia Rodrigues, AU - de Sá,Claudia Mara, AU - Freire,Maria Das Graças Machado, AU - Parra,José Roberto Postali, PY - 2004/4/29/pubmed PY - 2004/6/29/medline PY - 2004/4/29/entrez SP - 2533 EP - 40 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 9 N2 - The cowpea weevil Callosobruchus maculatus is one of the major pests of Vigna unguiculata cowpea. Digestion in the cowpea weevil is facilitated by high levels of cysteine and aspartic acid proteinases. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (ApTI) isolated from Adenanthera pavonina seeds showed activity against papain. The inhibition of papain by ApTI was of the noncompetitive type, with a K(i) of 1 microM. ApTI was highly effective against digestive proteinases from C. maculatus, Acanthoscelides obtectus (bean weevil), and Zabrotes subfasciatus (Mexican bean weevil) and was moderately active against midgut proteinases from the boll weevil Anthonomus grandis and the mealworm Tenebrio molitor. In C. maculates fed an artificial diet containing 0.25% and 0.5% ApTI (w/w), the latter concentration caused 50% mortality and reduced larval weight gain by approximately 40%. The action of ApTI on C. maculatus larvae may involve the inhibition of ApTI-sensitive cysteine proteinases and binding to chitin components of the peritrophic membrane (or equivalent structures) in the weevil midgut. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/15113152/A_Kunitz_type_inhibitor_of_coleopteran_proteases_isolated_from_Adenanthera_pavonina_L__seeds_and_its_effect_on_Callosobruchus_maculatus_ DB - PRIME DP - Unbound Medicine ER -
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