TY - JOUR T1 - Simple reversed-phase ion-pair liquid chromatography assay for the simultaneous determination of mycophenolic acid and its glucuronide metabolite in human plasma and urine. AU - Yau,Wai-Ping, AU - Vathsala,Anantharaman, AU - Lou,Huei-Xin, AU - Chan,Eli, PY - 2003/10/22/received PY - 2004/01/19/revised PY - 2004/02/16/accepted PY - 2004/4/29/pubmed PY - 2004/12/17/medline PY - 2004/4/29/entrez SP - 101 EP - 12 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 805 IS - 1 N2 - A simple and reproducible reversed-phase ion-pair high-performance liquid chromatographic (HPLC) method using isocratic elution with UV absorbance detection is presented for the simultaneous quantitation of mycophenolic acid (MPA) and MPA-glucuronide (MPAG) in human plasma and urine. The sample preparation procedures involved simple protein precipitation for plasma and 10-fold dilution for urine. Each analytical run was completed within 15min, with MPAG and MPA being eluted at 3.8 and 11.4min, respectively. The optimized method showed good performance in terms of specificity, linearity, detection and quantitation limits, precision and accuracy. This assay was demonstrated to be applicable for clinical pharmacokinetic studies. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15113545/Simple_reversed_phase_ion_pair_liquid_chromatography_assay_for_the_simultaneous_determination_of_mycophenolic_acid_and_its_glucuronide_metabolite_in_human_plasma_and_urine_ DB - PRIME DP - Unbound Medicine ER -