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Determination of Tirofiban in human serum by liquid chromatography-tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 05; 805(1):181-5.JC

Abstract

A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the determination of Tirofiban in biological fluids. Tirofiban in serum samples was extracted and cleaned up by using an automated solid phase extraction method. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode (MRM). A good linear response over the range of 2-200ng/ml was demonstrated. The accuracy for Tirofiban ranged from 94.8 to 110.8% within-day and from 103.0 to 104.7% between-day. The lower limit of quantification was 2ng/ml. This method is suitable for pharmacokinetic studies.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Fiedlerstr. 27, 01307 Dresden, Germany. reioer@rcs.urz.tu-dresden.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15113556

Citation

Oertel, R, et al. "Determination of Tirofiban in Human Serum By Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 805, no. 1, 2004, pp. 181-5.
Oertel R, Köhler A, Koster A, et al. Determination of Tirofiban in human serum by liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;805(1):181-5.
Oertel, R., Köhler, A., Koster, A., & Kirch, W. (2004). Determination of Tirofiban in human serum by liquid chromatography-tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 805(1), 181-5.
Oertel R, et al. Determination of Tirofiban in Human Serum By Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 5;805(1):181-5. PubMed PMID: 15113556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of Tirofiban in human serum by liquid chromatography-tandem mass spectrometry. AU - Oertel,R, AU - Köhler,A, AU - Koster,A, AU - Kirch,W, PY - 2003/11/20/received PY - 2004/02/02/revised PY - 2004/03/01/accepted PY - 2004/4/29/pubmed PY - 2004/12/17/medline PY - 2004/4/29/entrez SP - 181 EP - 5 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 805 IS - 1 N2 - A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the determination of Tirofiban in biological fluids. Tirofiban in serum samples was extracted and cleaned up by using an automated solid phase extraction method. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode (MRM). A good linear response over the range of 2-200ng/ml was demonstrated. The accuracy for Tirofiban ranged from 94.8 to 110.8% within-day and from 103.0 to 104.7% between-day. The lower limit of quantification was 2ng/ml. This method is suitable for pharmacokinetic studies. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15113556/Determination_of_Tirofiban_in_human_serum_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570023204002090 DB - PRIME DP - Unbound Medicine ER -