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Equine beta-defensin-1: full-length cDNA sequence and tissue expression.
Vet Immunol Immunopathol. 2004 May; 99(1-2):127-32.VI

Abstract

beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immune defense. Although, previous investigations have identified beta-defensins in several mammalian species, no reports have identified equine beta-defensins. Using a strategy of database searching for expressed sequence tags (EST) we identified putative expression of equine beta-defensins in hepatic tissue. Based on this information, sequence specific primers were designed for the equine gene enabling the identification of the full-length cDNA sequence of equine beta-defensin-1. Comparative analyses showed that equine beta-defensin-1 has 46-52% amino-acid identity with other beta-defensins, sharing the greatest identity with porcine beta-defensin-1. Complete conservation of cysteine residues was maintained between the species evaluated, and RT-PCR analysis revealed diverse mRNA tissue expression for equine beta-defensin-1. These data extend the repertoire of equine antimicrobial peptides and expand our understanding of equine innate immunity.

Authors+Show Affiliations

Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. edavis@vet.k-state.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15113660

Citation

Davis, Elizabeth G., et al. "Equine Beta-defensin-1: Full-length cDNA Sequence and Tissue Expression." Veterinary Immunology and Immunopathology, vol. 99, no. 1-2, 2004, pp. 127-32.
Davis EG, Sang Y, Blecha F. Equine beta-defensin-1: full-length cDNA sequence and tissue expression. Vet Immunol Immunopathol. 2004;99(1-2):127-32.
Davis, E. G., Sang, Y., & Blecha, F. (2004). Equine beta-defensin-1: full-length cDNA sequence and tissue expression. Veterinary Immunology and Immunopathology, 99(1-2), 127-32.
Davis EG, Sang Y, Blecha F. Equine Beta-defensin-1: Full-length cDNA Sequence and Tissue Expression. Vet Immunol Immunopathol. 2004;99(1-2):127-32. PubMed PMID: 15113660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Equine beta-defensin-1: full-length cDNA sequence and tissue expression. AU - Davis,Elizabeth G, AU - Sang,Yongming, AU - Blecha,Frank, PY - 2003/10/14/received PY - 2003/12/19/accepted PY - 2004/4/29/pubmed PY - 2004/7/28/medline PY - 2004/4/29/entrez SP - 127 EP - 32 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 99 IS - 1-2 N2 - beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immune defense. Although, previous investigations have identified beta-defensins in several mammalian species, no reports have identified equine beta-defensins. Using a strategy of database searching for expressed sequence tags (EST) we identified putative expression of equine beta-defensins in hepatic tissue. Based on this information, sequence specific primers were designed for the equine gene enabling the identification of the full-length cDNA sequence of equine beta-defensin-1. Comparative analyses showed that equine beta-defensin-1 has 46-52% amino-acid identity with other beta-defensins, sharing the greatest identity with porcine beta-defensin-1. Complete conservation of cysteine residues was maintained between the species evaluated, and RT-PCR analysis revealed diverse mRNA tissue expression for equine beta-defensin-1. These data extend the repertoire of equine antimicrobial peptides and expand our understanding of equine innate immunity. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/15113660/Equine_beta_defensin_1:_full_length_cDNA_sequence_and_tissue_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165242704000030 DB - PRIME DP - Unbound Medicine ER -