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Tyrosine hydroxylase and dopamine transporter expression following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurodegeneration of the mouse nigrostriatal pathway.
J Neurosci Res. 2004 May 15; 76(4):539-50.JN

Abstract

Administration of the neurotoxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to C57BL/6 mice targets nigrostriatal dopaminergic neurons, leading to cell death and the depletion of striatal dopamine. After MPTP lesioning in young adult mice, surviving nigrostriatal dopaminergic neurons display robust and reproducible return of striatal dopamine weeks to months after injury. Thus, the mouse provides an excellent model with which to investigate the mechanisms underlying neuroplasticity of the nigrostriatal system following neurotoxic injury. The purpose of this study was to analyze proteins and mRNA transcripts of genes involved in dopamine biosynthesis (tyrosine hydroxylase; TH) and uptake (dopamine transporter; DAT) with regard to time course (7-90 days) after MPTP lesioning. Molecular analysis using immunohistochemistry and Western immunoblotting techniques demonstrated an increase in striatal TH by 30-60 days postlesioning that returned to near-control (prelesioned) levels by 60-90 days. In situ hybridization histochemistry indicated that this increase in TH protein might be due in part to increased TH mRNA expression in surviving nigrostriatal dopaminergic neurons. Analysis of TH protein at 7, 30, 60, and 90 days postlesioning with two-dimensional polyacrylamide gel electrophoresis in conjunction with Western immunoblotting revealed altered TH protein isoforms migrating at isoelectric points different from those of the native isoform. In contrast to TH protein, which returned to prelesioned levels by 60 days, DAT protein analysis showed that increased expression of striatal DAT protein did not return to near-prelesion levels until 90 days postlesioning. These results suggest that TH and DAT may differ in their time course of expression in surviving dopaminergic neurons and may play a role in mediating the return of striatal dopamine.

Authors+Show Affiliations

Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA. mjakowec@surgery.usc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15114626

Citation

Jakowec, Michael W., et al. "Tyrosine Hydroxylase and Dopamine Transporter Expression Following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurodegeneration of the Mouse Nigrostriatal Pathway." Journal of Neuroscience Research, vol. 76, no. 4, 2004, pp. 539-50.
Jakowec MW, Nixon K, Hogg E, et al. Tyrosine hydroxylase and dopamine transporter expression following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurodegeneration of the mouse nigrostriatal pathway. J Neurosci Res. 2004;76(4):539-50.
Jakowec, M. W., Nixon, K., Hogg, E., McNeill, T., & Petzinger, G. M. (2004). Tyrosine hydroxylase and dopamine transporter expression following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurodegeneration of the mouse nigrostriatal pathway. Journal of Neuroscience Research, 76(4), 539-50.
Jakowec MW, et al. Tyrosine Hydroxylase and Dopamine Transporter Expression Following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurodegeneration of the Mouse Nigrostriatal Pathway. J Neurosci Res. 2004 May 15;76(4):539-50. PubMed PMID: 15114626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tyrosine hydroxylase and dopamine transporter expression following 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurodegeneration of the mouse nigrostriatal pathway. AU - Jakowec,Michael W, AU - Nixon,Kerry, AU - Hogg,Elizabeth, AU - McNeill,Tom, AU - Petzinger,Giselle M, PY - 2004/4/29/pubmed PY - 2004/6/23/medline PY - 2004/4/29/entrez SP - 539 EP - 50 JF - Journal of neuroscience research JO - J Neurosci Res VL - 76 IS - 4 N2 - Administration of the neurotoxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to C57BL/6 mice targets nigrostriatal dopaminergic neurons, leading to cell death and the depletion of striatal dopamine. After MPTP lesioning in young adult mice, surviving nigrostriatal dopaminergic neurons display robust and reproducible return of striatal dopamine weeks to months after injury. Thus, the mouse provides an excellent model with which to investigate the mechanisms underlying neuroplasticity of the nigrostriatal system following neurotoxic injury. The purpose of this study was to analyze proteins and mRNA transcripts of genes involved in dopamine biosynthesis (tyrosine hydroxylase; TH) and uptake (dopamine transporter; DAT) with regard to time course (7-90 days) after MPTP lesioning. Molecular analysis using immunohistochemistry and Western immunoblotting techniques demonstrated an increase in striatal TH by 30-60 days postlesioning that returned to near-control (prelesioned) levels by 60-90 days. In situ hybridization histochemistry indicated that this increase in TH protein might be due in part to increased TH mRNA expression in surviving nigrostriatal dopaminergic neurons. Analysis of TH protein at 7, 30, 60, and 90 days postlesioning with two-dimensional polyacrylamide gel electrophoresis in conjunction with Western immunoblotting revealed altered TH protein isoforms migrating at isoelectric points different from those of the native isoform. In contrast to TH protein, which returned to prelesioned levels by 60 days, DAT protein analysis showed that increased expression of striatal DAT protein did not return to near-prelesion levels until 90 days postlesioning. These results suggest that TH and DAT may differ in their time course of expression in surviving dopaminergic neurons and may play a role in mediating the return of striatal dopamine. SN - 0360-4012 UR - https://www.unboundmedicine.com/medline/citation/15114626/Tyrosine_hydroxylase_and_dopamine_transporter_expression_following_1_methyl_4_phenyl_1236_tetrahydropyridine_induced_neurodegeneration_of_the_mouse_nigrostriatal_pathway_ L2 - https://doi.org/10.1002/jnr.20114 DB - PRIME DP - Unbound Medicine ER -