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Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction.

Abstract

The early detection of scrub typhus in Thailand by nested polymerase chain reaction (PCR) is presented. The diagnosis of scrub typhus, from clinical samples obtained from hospitals in the northern part of Thailand, by nested PCR was compared to immunofluorescence (IF) and Weil-Felix (WF) tests. The primer pairs used for the nested PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen, and RFLP analysis was used for identification. Clotted blood from 80 patients suspected of scrub typhus infection were tested. With the IF test, antibodies for Orientia tsutsugamushi were observed in 38 patients checking IgM and IgG titers. Only 21 patients showed positive seroconversion while 17 patients were negative. For the WF test, only 13 patients gave a positive seroconversion. In the early stage of infection, 19, 13 and 3 patients were detected with a sensitivity of 90.47% (19/21), 61.90% (13/21) and 14.28% (3/21) by the nested PCR, IF and WF test respectively. Two patients who were negative for seroconvesion by IF and WF were positive by nested PCR. Therefore, this suggests that nested PCR is applicable for specific rapid diagnosis at an early stage of scrub typhus in endemic regions.

Authors+Show Affiliations

Pharmaceutical Cosmetic Raw Materials and Natural Products Research and Development Center, Institute for Science and Technology Research and Development/Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand. pmpti006@chiangmai.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15115096

Citation

Manosroi, Jiradej, et al. "Early Diagnosis of Scrub Typhus in Thailand From Clinical Specimens By Nested Polymerase Chain Reaction." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 34, no. 4, 2003, pp. 831-8.
Manosroi J, Chutipongvivate S, Auwanit W, et al. Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. Southeast Asian J Trop Med Public Health. 2003;34(4):831-8.
Manosroi, J., Chutipongvivate, S., Auwanit, W., & Manosroi, A. (2003). Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. The Southeast Asian Journal of Tropical Medicine and Public Health, 34(4), 831-8.
Manosroi J, et al. Early Diagnosis of Scrub Typhus in Thailand From Clinical Specimens By Nested Polymerase Chain Reaction. Southeast Asian J Trop Med Public Health. 2003;34(4):831-8. PubMed PMID: 15115096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early diagnosis of scrub typhus in Thailand from clinical specimens by nested polymerase chain reaction. AU - Manosroi,Jiradej, AU - Chutipongvivate,Salakchit, AU - Auwanit,Wattana, AU - Manosroi,Aranya, PY - 2004/4/30/pubmed PY - 2004/5/20/medline PY - 2004/4/30/entrez SP - 831 EP - 8 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 34 IS - 4 N2 - The early detection of scrub typhus in Thailand by nested polymerase chain reaction (PCR) is presented. The diagnosis of scrub typhus, from clinical samples obtained from hospitals in the northern part of Thailand, by nested PCR was compared to immunofluorescence (IF) and Weil-Felix (WF) tests. The primer pairs used for the nested PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen, and RFLP analysis was used for identification. Clotted blood from 80 patients suspected of scrub typhus infection were tested. With the IF test, antibodies for Orientia tsutsugamushi were observed in 38 patients checking IgM and IgG titers. Only 21 patients showed positive seroconversion while 17 patients were negative. For the WF test, only 13 patients gave a positive seroconversion. In the early stage of infection, 19, 13 and 3 patients were detected with a sensitivity of 90.47% (19/21), 61.90% (13/21) and 14.28% (3/21) by the nested PCR, IF and WF test respectively. Two patients who were negative for seroconvesion by IF and WF were positive by nested PCR. Therefore, this suggests that nested PCR is applicable for specific rapid diagnosis at an early stage of scrub typhus in endemic regions. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/15115096/Early_diagnosis_of_scrub_typhus_in_Thailand_from_clinical_specimens_by_nested_polymerase_chain_reaction_ L2 - http://www.diseaseinfosearch.org/result/7277 DB - PRIME DP - Unbound Medicine ER -