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Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals.
Rapid Commun Mass Spectrom. 2004; 18(9):923-30.RC

Abstract

We have developed a dried-droplet probe preparation method for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), which uses AnchorChip targets and alpha-cyano-4-hydroxycinnamic acid (CHCA) as a matrix. Upon drying of a matrix and analyte mixture on the AnchorChip, salts and low molecular weight contaminants were pooled at the hydrophilic metal anchor, whereas 10-50 microm matrix/peptide crystals firmly adhered at the surface of a hydrophobic polymer and the entire target could be subsequently washed by submerging it in 5% formic acid for 2-3 min. Epifluorescence microscopy suggested that peptides were completely co-localized with CHCA crystals at the AnchorChip surface. Fluorescent images of the probes were of good contrast and were background-free, compared with images taken by a video camera built into the ion source. CHCA/peptide crystals were easy to recognize at the surface and peptide mass maps were acquired from them without further adjustment of the position of the laser beam. These crystals were remarkably stable towards the laser depletion and almost no matrix-related ions were typically observed in the low m/z region of peptide mass maps. The sensitivity of the peptide mass mapping was at the low-femtomole level.

Authors+Show Affiliations

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15116417

Citation

Thomas, Henrik, et al. "Dried-droplet Probe Preparation On AnchorChip Targets for Navigating the Acquisition of Matrix-assisted Laser Desorption/ionization Time-of-flight Spectra By Fluorescence of Matrix/analyte Crystals." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 9, 2004, pp. 923-30.
Thomas H, Havlis J, Peychl J, et al. Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals. Rapid Commun Mass Spectrom. 2004;18(9):923-30.
Thomas, H., Havlis, J., Peychl, J., & Shevchenko, A. (2004). Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals. Rapid Communications in Mass Spectrometry : RCM, 18(9), 923-30.
Thomas H, et al. Dried-droplet Probe Preparation On AnchorChip Targets for Navigating the Acquisition of Matrix-assisted Laser Desorption/ionization Time-of-flight Spectra By Fluorescence of Matrix/analyte Crystals. Rapid Commun Mass Spectrom. 2004;18(9):923-30. PubMed PMID: 15116417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dried-droplet probe preparation on AnchorChip targets for navigating the acquisition of matrix-assisted laser desorption/ionization time-of-flight spectra by fluorescence of matrix/analyte crystals. AU - Thomas,Henrik, AU - Havlis,Jan, AU - Peychl,Jan, AU - Shevchenko,Andrej, PY - 2004/4/30/pubmed PY - 2004/5/27/medline PY - 2004/4/30/entrez SP - 923 EP - 30 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 9 N2 - We have developed a dried-droplet probe preparation method for peptide mass fingerprinting by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), which uses AnchorChip targets and alpha-cyano-4-hydroxycinnamic acid (CHCA) as a matrix. Upon drying of a matrix and analyte mixture on the AnchorChip, salts and low molecular weight contaminants were pooled at the hydrophilic metal anchor, whereas 10-50 microm matrix/peptide crystals firmly adhered at the surface of a hydrophobic polymer and the entire target could be subsequently washed by submerging it in 5% formic acid for 2-3 min. Epifluorescence microscopy suggested that peptides were completely co-localized with CHCA crystals at the AnchorChip surface. Fluorescent images of the probes were of good contrast and were background-free, compared with images taken by a video camera built into the ion source. CHCA/peptide crystals were easy to recognize at the surface and peptide mass maps were acquired from them without further adjustment of the position of the laser beam. These crystals were remarkably stable towards the laser depletion and almost no matrix-related ions were typically observed in the low m/z region of peptide mass maps. The sensitivity of the peptide mass mapping was at the low-femtomole level. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15116417/Dried_droplet_probe_preparation_on_AnchorChip_targets_for_navigating_the_acquisition_of_matrix_assisted_laser_desorption/ionization_time_of_flight_spectra_by_fluorescence_of_matrix/analyte_crystals_ DB - PRIME DP - Unbound Medicine ER -