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Qualitative analysis and HPLC isolation and identification of procyanidins from Vicia faba.
Phytochem Anal. 2004 Mar-Apr; 15(2):95-9.PA

Abstract

The soluble proanthocyanidins of the coloured seed coats of Vicia faba L. were isolated and separated by solvent partition. The chemical characteristics of the proanthocyanidins were elucidated by total oxidation and partial degradation in the presence of phloroglucinol followed by HPLC analysis. The native extract of proanthocyanidins contained (+)-gallocatechin, (-)-epigallocatechin, (+)-catechin and (-)-epicatechin units. Oligomeric procyanidins were purified by chromatography on Sephadex LH-20 and the accessible compounds were isolated by RP-HPLC using a Licrospher Li 100 Column. The structures of the purified oligomeric procyanidins were elucidated using a procedure involving TLC, UV spectroscopy, ESI-MS and HPLC analysis of the products from the phloroglucinol reaction. The major condensed tannins of Vicia faba comprise six compounds identified as two A-type procyanidin dimers, the procyanidin dimers B1, B2 and B3, and a procyanidin trimer.

Authors+Show Affiliations

UFR de Biologie, Laboratoire de Phytochimie, Båt F. A. Forel, Université Claude Bernard Lyon 1, F.69622 Villeurbanne cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15116939

Citation

Merghem, Rachid, et al. "Qualitative Analysis and HPLC Isolation and Identification of Procyanidins From Vicia Faba." Phytochemical Analysis : PCA, vol. 15, no. 2, 2004, pp. 95-9.
Merghem R, Jay M, Brun N, et al. Qualitative analysis and HPLC isolation and identification of procyanidins from Vicia faba. Phytochem Anal. 2004;15(2):95-9.
Merghem, R., Jay, M., Brun, N., & Voirin, B. (2004). Qualitative analysis and HPLC isolation and identification of procyanidins from Vicia faba. Phytochemical Analysis : PCA, 15(2), 95-9.
Merghem R, et al. Qualitative Analysis and HPLC Isolation and Identification of Procyanidins From Vicia Faba. Phytochem Anal. 2004;15(2):95-9. PubMed PMID: 15116939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Qualitative analysis and HPLC isolation and identification of procyanidins from Vicia faba. AU - Merghem,Rachid, AU - Jay,Maurice, AU - Brun,Nathalie, AU - Voirin,Bernard, PY - 2004/5/1/pubmed PY - 2004/6/23/medline PY - 2004/5/1/entrez SP - 95 EP - 9 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 15 IS - 2 N2 - The soluble proanthocyanidins of the coloured seed coats of Vicia faba L. were isolated and separated by solvent partition. The chemical characteristics of the proanthocyanidins were elucidated by total oxidation and partial degradation in the presence of phloroglucinol followed by HPLC analysis. The native extract of proanthocyanidins contained (+)-gallocatechin, (-)-epigallocatechin, (+)-catechin and (-)-epicatechin units. Oligomeric procyanidins were purified by chromatography on Sephadex LH-20 and the accessible compounds were isolated by RP-HPLC using a Licrospher Li 100 Column. The structures of the purified oligomeric procyanidins were elucidated using a procedure involving TLC, UV spectroscopy, ESI-MS and HPLC analysis of the products from the phloroglucinol reaction. The major condensed tannins of Vicia faba comprise six compounds identified as two A-type procyanidin dimers, the procyanidin dimers B1, B2 and B3, and a procyanidin trimer. SN - 0958-0344 UR - https://www.unboundmedicine.com/medline/citation/15116939/Qualitative_analysis_and_HPLC_isolation_and_identification_of_procyanidins_from_Vicia_faba_ L2 - https://doi.org/10.1002/pca.731 DB - PRIME DP - Unbound Medicine ER -