Tags

Type your tag names separated by a space and hit enter

Orally administered rosmarinic acid is present as the conjugated and/or methylated forms in plasma, and is degraded and metabolized to conjugated forms of caffeic acid, ferulic acid and m-coumaric acid.
Life Sci. 2004 May 28; 75(2):165-78.LS

Abstract

Rosmarinic acid (RA) is contained in various Lamiaceae herbs used commonly as culinary herbs. Although RA has various potent physiological actions, little is known on its bioavailability. We therefore investigated the absorption and metabolism of orally administered RA in rats. After being deprived of food for 12 h, RA (50 mg/kg body weight) or deionized water was administered orally to rats. Blood samples were collected from a cannula inserted in the femoral artery before and at designated time intervals after administration of RA. Urine excreted within 0 to 8 h and 8 to 18 h post-administration was also collected. RA and its related metabolites in plasma and urine were measured by LC-MS after treatment with sulfatase and/or beta-glucuronidase. RA, mono-methylated RA (methyl-RA) and m-coumaric acid (COA) were detected in plasma, with peak concentrations being reached at 0.5, 1 and 8 h after RA administration, respectively. RA, methyl-RA, caffeic acid (CAA), ferulic acid (FA) and COA were detected in urine after RA administration. These components in plasma and urine were present predominantly as conjugated forms such as glucuronide or sulfate. The percentage of the original oral dose of RA excreted in the urine within 18 h of administration as free and conjugated forms was 0.44 +/- 0.21% for RA, 1.60 +/- 0.74% for methyl-RA, 1.06 +/- 0.35% for CAA, 1.70 +/- 0.45% for FA and 0.67 +/- 0.29% for COA. Approximately 83% of the total amount of these metabolites was excreted in the period 8 to 18 h after RA administration. These results suggest that RA was absorbed and metabolized as conjugated and/or methylated forms, and that the majority of RA absorbed was degraded into conjugated and/or methylated forms of CAA, FA and COA before being excreted gradually in the urine.

Authors+Show Affiliations

Nutritional Science Center, Health and Bioscience Laboratories, Meiji Seika Kaisha, Ltd, 5-3-1, Chiyoda, Sakado-shi, Saitama 350-0289, Japan. seigo_baba@meiji.co.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15120569

Citation

Baba, Seigo, et al. "Orally Administered Rosmarinic Acid Is Present as the Conjugated And/or Methylated Forms in Plasma, and Is Degraded and Metabolized to Conjugated Forms of Caffeic Acid, Ferulic Acid and M-coumaric Acid." Life Sciences, vol. 75, no. 2, 2004, pp. 165-78.
Baba S, Osakabe N, Natsume M, et al. Orally administered rosmarinic acid is present as the conjugated and/or methylated forms in plasma, and is degraded and metabolized to conjugated forms of caffeic acid, ferulic acid and m-coumaric acid. Life Sci. 2004;75(2):165-78.
Baba, S., Osakabe, N., Natsume, M., & Terao, J. (2004). Orally administered rosmarinic acid is present as the conjugated and/or methylated forms in plasma, and is degraded and metabolized to conjugated forms of caffeic acid, ferulic acid and m-coumaric acid. Life Sciences, 75(2), 165-78.
Baba S, et al. Orally Administered Rosmarinic Acid Is Present as the Conjugated And/or Methylated Forms in Plasma, and Is Degraded and Metabolized to Conjugated Forms of Caffeic Acid, Ferulic Acid and M-coumaric Acid. Life Sci. 2004 May 28;75(2):165-78. PubMed PMID: 15120569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Orally administered rosmarinic acid is present as the conjugated and/or methylated forms in plasma, and is degraded and metabolized to conjugated forms of caffeic acid, ferulic acid and m-coumaric acid. AU - Baba,Seigo, AU - Osakabe,Naomi, AU - Natsume,Midori, AU - Terao,Junji, PY - 2003/09/24/received PY - 2003/11/19/accepted PY - 2004/5/4/pubmed PY - 2004/6/23/medline PY - 2004/5/4/entrez SP - 165 EP - 78 JF - Life sciences JO - Life Sci VL - 75 IS - 2 N2 - Rosmarinic acid (RA) is contained in various Lamiaceae herbs used commonly as culinary herbs. Although RA has various potent physiological actions, little is known on its bioavailability. We therefore investigated the absorption and metabolism of orally administered RA in rats. After being deprived of food for 12 h, RA (50 mg/kg body weight) or deionized water was administered orally to rats. Blood samples were collected from a cannula inserted in the femoral artery before and at designated time intervals after administration of RA. Urine excreted within 0 to 8 h and 8 to 18 h post-administration was also collected. RA and its related metabolites in plasma and urine were measured by LC-MS after treatment with sulfatase and/or beta-glucuronidase. RA, mono-methylated RA (methyl-RA) and m-coumaric acid (COA) were detected in plasma, with peak concentrations being reached at 0.5, 1 and 8 h after RA administration, respectively. RA, methyl-RA, caffeic acid (CAA), ferulic acid (FA) and COA were detected in urine after RA administration. These components in plasma and urine were present predominantly as conjugated forms such as glucuronide or sulfate. The percentage of the original oral dose of RA excreted in the urine within 18 h of administration as free and conjugated forms was 0.44 +/- 0.21% for RA, 1.60 +/- 0.74% for methyl-RA, 1.06 +/- 0.35% for CAA, 1.70 +/- 0.45% for FA and 0.67 +/- 0.29% for COA. Approximately 83% of the total amount of these metabolites was excreted in the period 8 to 18 h after RA administration. These results suggest that RA was absorbed and metabolized as conjugated and/or methylated forms, and that the majority of RA absorbed was degraded into conjugated and/or methylated forms of CAA, FA and COA before being excreted gradually in the urine. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/15120569/Orally_administered_rosmarinic_acid_is_present_as_the_conjugated_and/or_methylated_forms_in_plasma_and_is_degraded_and_metabolized_to_conjugated_forms_of_caffeic_acid_ferulic_acid_and_m_coumaric_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024320504002231 DB - PRIME DP - Unbound Medicine ER -