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Steatosis and liver cell apoptosis in chronic hepatitis C: a mechanism for increased liver injury.
Hepatology. 2004 May; 39(5):1230-8.Hep

Abstract

Steatosis is increasingly recognized as a cofactor influencing the progression of fibrosis in chronic hepatitis C; however, the mechanisms by which it contributes to liver injury remain uncertain. We studied 125 patients with chronic hepatitis C to assess the effect of steatosis on liver cell apoptosis and the expression of Bcl-2, Bcl-x(L), Bax, and tumor necrosis factor alpha (TNF-alpha) and the relationship between liver cell apoptosis and disease severity. A significant increase in liver cell apoptosis was seen in liver sections with increasing grade of steatosis (r = 0.42; P <.0001). Hepatic steatosis and previous heavy alcohol consumption were the only two variables independently associated with the apoptotic index. Increasing steatosis was associated with decreased Bcl-2 mRNA levels and an increase in the proapoptotic Bax/Bcl-2 ratio (r = -0.32, P =.007; and r = 0.27, P =.02, respectively). In the absence of steatosis, increased liver cell apoptosis was not associated with stellate cell activation or fibrosis (r = 0.26, P =.11; r = 0.06, P =.71, respectively). In contrast, in the presence of steatosis, increasing apoptosis was associated with activation of stellate cells and increased stage of fibrosis (r = 0.35, P =.047; r = 0.33, P =.03, respectively), supporting the premise that the steatotic liver is more vulnerable to liver injury. In patients with hepatitis C virus genotype 3, there was a significant correlation between TNF-alpha mRNA levels and active caspase-3 (r = 0.54, P =.007). In conclusion, these observations suggest a mechanism whereby steatosis contributes to the progression of liver injury in chronic hepatitis C. Further investigation will be required to determine the molecular pathways responsible for the proapoptotic effect of steatosis and whether this increase in apoptosis contributes directly to fibrogenesis.

Authors+Show Affiliations

School of Medicine, Southern Division, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15122751

Citation

Walsh, Meagan J., et al. "Steatosis and Liver Cell Apoptosis in Chronic Hepatitis C: a Mechanism for Increased Liver Injury." Hepatology (Baltimore, Md.), vol. 39, no. 5, 2004, pp. 1230-8.
Walsh MJ, Vanags DM, Clouston AD, et al. Steatosis and liver cell apoptosis in chronic hepatitis C: a mechanism for increased liver injury. Hepatology. 2004;39(5):1230-8.
Walsh, M. J., Vanags, D. M., Clouston, A. D., Richardson, M. M., Purdie, D. M., Jonsson, J. R., & Powell, E. E. (2004). Steatosis and liver cell apoptosis in chronic hepatitis C: a mechanism for increased liver injury. Hepatology (Baltimore, Md.), 39(5), 1230-8.
Walsh MJ, et al. Steatosis and Liver Cell Apoptosis in Chronic Hepatitis C: a Mechanism for Increased Liver Injury. Hepatology. 2004;39(5):1230-8. PubMed PMID: 15122751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Steatosis and liver cell apoptosis in chronic hepatitis C: a mechanism for increased liver injury. AU - Walsh,Meagan J, AU - Vanags,Daina M, AU - Clouston,Andrew D, AU - Richardson,Michelle M, AU - Purdie,David M, AU - Jonsson,Julie R, AU - Powell,Elizabeth E, PY - 2004/5/4/pubmed PY - 2004/5/25/medline PY - 2004/5/4/entrez SP - 1230 EP - 8 JF - Hepatology (Baltimore, Md.) JO - Hepatology VL - 39 IS - 5 N2 - Steatosis is increasingly recognized as a cofactor influencing the progression of fibrosis in chronic hepatitis C; however, the mechanisms by which it contributes to liver injury remain uncertain. We studied 125 patients with chronic hepatitis C to assess the effect of steatosis on liver cell apoptosis and the expression of Bcl-2, Bcl-x(L), Bax, and tumor necrosis factor alpha (TNF-alpha) and the relationship between liver cell apoptosis and disease severity. A significant increase in liver cell apoptosis was seen in liver sections with increasing grade of steatosis (r = 0.42; P <.0001). Hepatic steatosis and previous heavy alcohol consumption were the only two variables independently associated with the apoptotic index. Increasing steatosis was associated with decreased Bcl-2 mRNA levels and an increase in the proapoptotic Bax/Bcl-2 ratio (r = -0.32, P =.007; and r = 0.27, P =.02, respectively). In the absence of steatosis, increased liver cell apoptosis was not associated with stellate cell activation or fibrosis (r = 0.26, P =.11; r = 0.06, P =.71, respectively). In contrast, in the presence of steatosis, increasing apoptosis was associated with activation of stellate cells and increased stage of fibrosis (r = 0.35, P =.047; r = 0.33, P =.03, respectively), supporting the premise that the steatotic liver is more vulnerable to liver injury. In patients with hepatitis C virus genotype 3, there was a significant correlation between TNF-alpha mRNA levels and active caspase-3 (r = 0.54, P =.007). In conclusion, these observations suggest a mechanism whereby steatosis contributes to the progression of liver injury in chronic hepatitis C. Further investigation will be required to determine the molecular pathways responsible for the proapoptotic effect of steatosis and whether this increase in apoptosis contributes directly to fibrogenesis. SN - 0270-9139 UR - https://www.unboundmedicine.com/medline/citation/15122751/Steatosis_and_liver_cell_apoptosis_in_chronic_hepatitis_C:_a_mechanism_for_increased_liver_injury_ L2 - https://doi.org/10.1002/hep.20179 DB - PRIME DP - Unbound Medicine ER -