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Characterization of monospecies biofilm formation by Helicobacter pylori.
J Bacteriol. 2004 May; 186(10):3124-32.JB

Abstract

As all bacteria studied to date, the gastric pathogen Helicobacter pylori has an alternate lifestyle as a biofilm. H. pylori forms biofilms on glass surfaces at the air-liquid interface in stationary or shaking batch cultures. By light microscopy, we have observed attachment of individual, spiral H. pylori to glass surfaces, followed by division to form microcolonies, merging of individual microcolonies, and growth in the third dimension. Scanning electron micrographs showed H. pylori arranged in a matrix on the glass with channels for nutrient flow, typical of other bacterial biofilms. To understand the importance of biofilms to the H. pylori life cycle, we tested the effect of mucin on biofilm formation. Our results showed that 10% mucin greatly increased the number of planktonic H. pylori while not affecting biofilm bacteria, resulting in a decline in percent adherence to the glass. This suggests that in the mucus-rich stomach, H. pylori planktonic growth is favored over biofilm formation. We also investigated the effect of specific mutations in several genes, including the quorum-sensing gene, luxS, and the cagE type IV secretion gene. Both of these mutants were found to form biofilms approximately twofold more efficiently than the wild type in both assays. These results indicate the relative importance of these genes to the production of biofilms by H. pylori and the selective enhancement of planktonic growth in the presence of gastric mucin.

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Authors+Show Affiliations

Cole SP
Department of Medicine, University of California-San Diego, La Jolla, California 92093-0640, USA.
Harwood J
No affiliation info available
Lee R
No affiliation info available
She R
No affiliation info available
Guiney DG
No affiliation info available

MeSH

Bacterial AdhesionBacterial ProteinsBiofilmsCarbon-Sulfur LyasesHelicobacter pyloriMucins

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15126474

Citation

Cole, Sheri P., et al. "Characterization of Monospecies Biofilm Formation By Helicobacter Pylori." Journal of Bacteriology, vol. 186, no. 10, 2004, pp. 3124-32.
Cole SP, Harwood J, Lee R, et al. Characterization of monospecies biofilm formation by Helicobacter pylori. J Bacteriol. 2004;186(10):3124-32.
Cole, S. P., Harwood, J., Lee, R., She, R., & Guiney, D. G. (2004). Characterization of monospecies biofilm formation by Helicobacter pylori. Journal of Bacteriology, 186(10), 3124-32.
Cole SP, et al. Characterization of Monospecies Biofilm Formation By Helicobacter Pylori. J Bacteriol. 2004;186(10):3124-32. PubMed PMID: 15126474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of monospecies biofilm formation by Helicobacter pylori. AU - Cole,Sheri P, AU - Harwood,Julia, AU - Lee,Richard, AU - She,Rosemary, AU - Guiney,Donald G, PY - 2004/5/6/pubmed PY - 2004/6/21/medline PY - 2004/5/6/entrez SP - 3124 EP - 32 JF - Journal of bacteriology JO - J Bacteriol VL - 186 IS - 10 N2 - As all bacteria studied to date, the gastric pathogen Helicobacter pylori has an alternate lifestyle as a biofilm. H. pylori forms biofilms on glass surfaces at the air-liquid interface in stationary or shaking batch cultures. By light microscopy, we have observed attachment of individual, spiral H. pylori to glass surfaces, followed by division to form microcolonies, merging of individual microcolonies, and growth in the third dimension. Scanning electron micrographs showed H. pylori arranged in a matrix on the glass with channels for nutrient flow, typical of other bacterial biofilms. To understand the importance of biofilms to the H. pylori life cycle, we tested the effect of mucin on biofilm formation. Our results showed that 10% mucin greatly increased the number of planktonic H. pylori while not affecting biofilm bacteria, resulting in a decline in percent adherence to the glass. This suggests that in the mucus-rich stomach, H. pylori planktonic growth is favored over biofilm formation. We also investigated the effect of specific mutations in several genes, including the quorum-sensing gene, luxS, and the cagE type IV secretion gene. Both of these mutants were found to form biofilms approximately twofold more efficiently than the wild type in both assays. These results indicate the relative importance of these genes to the production of biofilms by H. pylori and the selective enhancement of planktonic growth in the presence of gastric mucin. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/15126474/Characterization_of_monospecies_biofilm_formation_by_Helicobacter_pylori_ DB - PRIME DP - Unbound Medicine ER -