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Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line.
Laryngoscope. 2004 May; 114(5):855-9.L

Abstract

BACKGROUND/OBJECTIVES

Nasopharyngeal carcinoma (NPC) is a highly invasive and metastatic malignant tumor and is associated with Epstein-Barr virus (EBV) infection that exhibits type II latency. Angiogenesis is essential for tumor growth, invasion, and metastasis. Our previous studies have indicated that interleukin (IL)-8 was over-expressed in many NPC tissues and was found to be significantly correlated with angiogenesis by immunohistochemistry.

STUDY DESIGN

In vitro design.

METHODS

The influence of the EBV genome for IL-8 gene expression was studied using the EBV-genome-positive and -negative epithelial/NPC hybrid cell line NPC-KT. The EBV-positive and -negative clones were selected by polymerase chain reaction and in situ hybridization.

RESULTS

EBV-positive clones expressed abundant IL-8 mRNA compared with EBV-negative clones. This result indicated that over-expression of IL-8 depended on the presence of EBV genomes in NPC-KT cells. Two encoded genes, latent membrane protein (LMP)1 and EBV-encoded small RNAs (EBERs), expressed in NPC were transfected in EBV-negative NPC-KT cells. LMP1 transactivated the IL-8 promoter, whereas EBERs did not. Moreover, the nuclear factor (NF)-kappa B binding site in the IL-8 promoter was essential for the response to LMP1, and the activator protein (AP)-1 binding site played only a partial role.

CONCLUSIONS

LMP1 induces IL-8 mainly through the activation of NF-kappa B and partly through AP-1 in NPC model cell lines, NPC-KT, and this suggests that LMP1 plays an important role in the angiogenesis of NPC.

Authors+Show Affiliations

Division of Otolaryngology, Graduate School of Medicine, and Department of Molecular Oncology and Virology, Cancer Research Institute, Kanazawa University, Takaramachi, Kanazawa, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15126743

Citation

Ren, Qingchun, et al. "Epstein-Barr Virus (EBV) Latent Membrane Protein 1 Induces Interleukin-8 Through the Nuclear Factor-kappa B Signaling Pathway in EBV-infected Nasopharyngeal Carcinoma Cell Line." The Laryngoscope, vol. 114, no. 5, 2004, pp. 855-9.
Ren Q, Sato H, Murono S, et al. Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line. Laryngoscope. 2004;114(5):855-9.
Ren, Q., Sato, H., Murono, S., Furukawa, M., & Yoshizaki, T. (2004). Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line. The Laryngoscope, 114(5), 855-9.
Ren Q, et al. Epstein-Barr Virus (EBV) Latent Membrane Protein 1 Induces Interleukin-8 Through the Nuclear Factor-kappa B Signaling Pathway in EBV-infected Nasopharyngeal Carcinoma Cell Line. Laryngoscope. 2004;114(5):855-9. PubMed PMID: 15126743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line. AU - Ren,Qingchun, AU - Sato,Hiroshi, AU - Murono,Shigeyuki, AU - Furukawa,Mitsuru, AU - Yoshizaki,Tomokazu, PY - 2004/5/6/pubmed PY - 2004/6/24/medline PY - 2004/5/6/entrez SP - 855 EP - 9 JF - The Laryngoscope JO - Laryngoscope VL - 114 IS - 5 N2 - BACKGROUND/OBJECTIVES: Nasopharyngeal carcinoma (NPC) is a highly invasive and metastatic malignant tumor and is associated with Epstein-Barr virus (EBV) infection that exhibits type II latency. Angiogenesis is essential for tumor growth, invasion, and metastasis. Our previous studies have indicated that interleukin (IL)-8 was over-expressed in many NPC tissues and was found to be significantly correlated with angiogenesis by immunohistochemistry. STUDY DESIGN: In vitro design. METHODS: The influence of the EBV genome for IL-8 gene expression was studied using the EBV-genome-positive and -negative epithelial/NPC hybrid cell line NPC-KT. The EBV-positive and -negative clones were selected by polymerase chain reaction and in situ hybridization. RESULTS: EBV-positive clones expressed abundant IL-8 mRNA compared with EBV-negative clones. This result indicated that over-expression of IL-8 depended on the presence of EBV genomes in NPC-KT cells. Two encoded genes, latent membrane protein (LMP)1 and EBV-encoded small RNAs (EBERs), expressed in NPC were transfected in EBV-negative NPC-KT cells. LMP1 transactivated the IL-8 promoter, whereas EBERs did not. Moreover, the nuclear factor (NF)-kappa B binding site in the IL-8 promoter was essential for the response to LMP1, and the activator protein (AP)-1 binding site played only a partial role. CONCLUSIONS: LMP1 induces IL-8 mainly through the activation of NF-kappa B and partly through AP-1 in NPC model cell lines, NPC-KT, and this suggests that LMP1 plays an important role in the angiogenesis of NPC. SN - 0023-852X UR - https://www.unboundmedicine.com/medline/citation/15126743/Epstein_Barr_virus__EBV__latent_membrane_protein_1_induces_interleukin_8_through_the_nuclear_factor_kappa_B_signaling_pathway_in_EBV_infected_nasopharyngeal_carcinoma_cell_line_ L2 - https://doi.org/10.1097/00005537-200405000-00012 DB - PRIME DP - Unbound Medicine ER -