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Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes.
Sheng Li Xue Bao. 2004 Apr 25; 56(2):204-9.SL

Abstract

The effects of genistein (GST) on intracellular calcium concentration ([Ca(2+)](i)) were investigated in guinea pig ventricular myocytes. [Ca(2+)](i) was detected by confocal microscopy and represented by relative fluorescent intensity (FI-F(0)) /FI(0), %). The results showed that GST (10-40 micromol/L) reduced [Ca(2+)](i) in normal Tyrode's solution, Ca(2+)-free Tyrode's solution and normal Tyrode's solution containing 3 mmol/L EGTA in a concentration-dependent manner. The effects of GST on [Ca(2+)](i) in normal Tyrode's solution were partially inhibited by pretreatment with sodium orthovanadate, a potent inhibitor of protein tyrosine phosphatase, or L-type Ca(2+) channel agonist Bay K8644. GST also markedly inhibited the ryanodine-induced [Ca(2+)](i) responses in Ca(2+)-free Tyrode's solution. When Ca(2+) waves were produced by increasing extracellular Ca(2+) concentration from 1 to 10 mmol/L, GST (40 micromol/L) could block the propagating waves of elevated [Ca(2+)](i), and reduce the velocity and duration of propagating waves. These results suggest that GST may reduce the [Ca(2+)](i) in isolated guinea pig ventricular myocytes. The inhibition of voltage-dependent Ca(2+) channel, tyrosine kinase inhibition, and alleviation of Ca(2+) release from SR are possibly involved in the GST effect.

Authors+Show Affiliations

Departments of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15127131

Citation

Ji, En-Sheng, et al. "Effects of Genistein On Intracellular Free-calcium Concentration in Guinea Pig Ventricular Myocytes." Sheng Li Xue Bao : [Acta Physiologica Sinica], vol. 56, no. 2, 2004, pp. 204-9.
Ji ES, Wang C, He RR. Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes. Sheng Li Xue Bao. 2004;56(2):204-9.
Ji, E. S., Wang, C., & He, R. R. (2004). Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes. Sheng Li Xue Bao : [Acta Physiologica Sinica], 56(2), 204-9.
Ji ES, Wang C, He RR. Effects of Genistein On Intracellular Free-calcium Concentration in Guinea Pig Ventricular Myocytes. Sheng Li Xue Bao. 2004 Apr 25;56(2):204-9. PubMed PMID: 15127131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of genistein on intracellular free-calcium concentration in guinea pig ventricular myocytes. AU - Ji,En-Sheng, AU - Wang,Chuan, AU - He,Rui-Rong, PY - 2004/5/6/pubmed PY - 2004/7/23/medline PY - 2004/5/6/entrez SP - 204 EP - 9 JF - Sheng li xue bao : [Acta physiologica Sinica] JO - Sheng Li Xue Bao VL - 56 IS - 2 N2 - The effects of genistein (GST) on intracellular calcium concentration ([Ca(2+)](i)) were investigated in guinea pig ventricular myocytes. [Ca(2+)](i) was detected by confocal microscopy and represented by relative fluorescent intensity (FI-F(0)) /FI(0), %). The results showed that GST (10-40 micromol/L) reduced [Ca(2+)](i) in normal Tyrode's solution, Ca(2+)-free Tyrode's solution and normal Tyrode's solution containing 3 mmol/L EGTA in a concentration-dependent manner. The effects of GST on [Ca(2+)](i) in normal Tyrode's solution were partially inhibited by pretreatment with sodium orthovanadate, a potent inhibitor of protein tyrosine phosphatase, or L-type Ca(2+) channel agonist Bay K8644. GST also markedly inhibited the ryanodine-induced [Ca(2+)](i) responses in Ca(2+)-free Tyrode's solution. When Ca(2+) waves were produced by increasing extracellular Ca(2+) concentration from 1 to 10 mmol/L, GST (40 micromol/L) could block the propagating waves of elevated [Ca(2+)](i), and reduce the velocity and duration of propagating waves. These results suggest that GST may reduce the [Ca(2+)](i) in isolated guinea pig ventricular myocytes. The inhibition of voltage-dependent Ca(2+) channel, tyrosine kinase inhibition, and alleviation of Ca(2+) release from SR are possibly involved in the GST effect. SN - 0371-0874 UR - https://www.unboundmedicine.com/medline/citation/15127131/Effects_of_genistein_on_intracellular_free_calcium_concentration_in_guinea_pig_ventricular_myocytes_ DB - PRIME DP - Unbound Medicine ER -