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Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes.
Sheng Li Xue Bao. 2004 Apr 25; 56(2):243-7.SL

Abstract

The purpose of this study was to examine the effects of low-dose capsaicin (CAP) on L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes and the underlying mechanism. I(Ca-L) was examined in isolated single guinea pig ventricular myocytes by using whole-cell patch clamp technique. CAP (1-25 nmol/L) increased the voltage-dependently activated peak amplitude of I(Ca-L) and downshifted the current-voltage (I-V) curve. CAP (1, 10, 25 nmol/L) increased the peak amplitude of I(Ca-L) from -9.67+/-0.7 pA/pF to -10.21+/-0.8 pA/pF (P>0.05), to -11.37+/-0.8 pA/pF and to -12.84+/-0.9 pA/pF (P<0.05), respectively. CAP 25 nmol/L shifted the steady-state activation curve of I(Ca-L) to the left and changed half activation potential (V(0.5)) from (-20.76+/-2.0) mV to (-26.71+/-3.0) mV (P<0.05), indicating that low-dose CAP may modify the voltage-dependent activation of calcium channel. Low-dose of CAP did not affect the steady-state inactivation curve of I(Ca-L) or half-recovery time of Ca(2+) channel from inactivation. Ruthenium red (RR, 10 micromol/L), a vanilloid receptor (VR1) blocker, antagonized the effects of low-dose CAP. These results suggest that low-dose CAP increases I(Ca-L) mainly by shifting its steady-state activation curve to the left. Such effects may be mediated by VR1.

Authors+Show Affiliations

Cheng YP
Department of Physiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China.
Yin JX
No affiliation info available
Cheng LP
No affiliation info available
He RR
No affiliation info available

MeSH

AnimalsCalcium Channels, L-TypeCapsaicinDose-Response Relationship, DrugGuinea PigsHeart VentriclesMyocytes, CardiacPatch-Clamp TechniquesReceptors, DrugRuthenium Red

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15127137

Citation

Cheng, Yan-Ping, et al. "Effects of Low-dose Capsaicin On L-type Calcium Current in Guinea Pig Ventricular Myocytes." Sheng Li Xue Bao : [Acta Physiologica Sinica], vol. 56, no. 2, 2004, pp. 243-7.
Cheng YP, Yin JX, Cheng LP, et al. Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes. Sheng Li Xue Bao. 2004;56(2):243-7.
Cheng, Y. P., Yin, J. X., Cheng, L. P., & He, R. R. (2004). Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes. Sheng Li Xue Bao : [Acta Physiologica Sinica], 56(2), 243-7.
Cheng YP, et al. Effects of Low-dose Capsaicin On L-type Calcium Current in Guinea Pig Ventricular Myocytes. Sheng Li Xue Bao. 2004 Apr 25;56(2):243-7. PubMed PMID: 15127137.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of low-dose capsaicin on L-type calcium current in guinea pig ventricular myocytes. AU - Cheng,Yan-Ping, AU - Yin,Jing-Xiang, AU - Cheng,Li-Ping, AU - He,Rui-Rong, PY - 2004/5/6/pubmed PY - 2004/7/23/medline PY - 2004/5/6/entrez SP - 243 EP - 7 JF - Sheng li xue bao : [Acta physiologica Sinica] JO - Sheng Li Xue Bao VL - 56 IS - 2 N2 - The purpose of this study was to examine the effects of low-dose capsaicin (CAP) on L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes and the underlying mechanism. I(Ca-L) was examined in isolated single guinea pig ventricular myocytes by using whole-cell patch clamp technique. CAP (1-25 nmol/L) increased the voltage-dependently activated peak amplitude of I(Ca-L) and downshifted the current-voltage (I-V) curve. CAP (1, 10, 25 nmol/L) increased the peak amplitude of I(Ca-L) from -9.67+/-0.7 pA/pF to -10.21+/-0.8 pA/pF (P>0.05), to -11.37+/-0.8 pA/pF and to -12.84+/-0.9 pA/pF (P<0.05), respectively. CAP 25 nmol/L shifted the steady-state activation curve of I(Ca-L) to the left and changed half activation potential (V(0.5)) from (-20.76+/-2.0) mV to (-26.71+/-3.0) mV (P<0.05), indicating that low-dose CAP may modify the voltage-dependent activation of calcium channel. Low-dose of CAP did not affect the steady-state inactivation curve of I(Ca-L) or half-recovery time of Ca(2+) channel from inactivation. Ruthenium red (RR, 10 micromol/L), a vanilloid receptor (VR1) blocker, antagonized the effects of low-dose CAP. These results suggest that low-dose CAP increases I(Ca-L) mainly by shifting its steady-state activation curve to the left. Such effects may be mediated by VR1. SN - 0371-0874 UR - https://www.unboundmedicine.com/medline/citation/15127137/Effects_of_low_dose_capsaicin_on_L_type_calcium_current_in_guinea_pig_ventricular_myocytes_ DB - PRIME DP - Unbound Medicine ER -