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Effect of controlled zinc release on bone mineral density from injectable Zn-containing beta-tricalcium phosphate suspension in zinc-deficient diseased rats.
J Biomed Mater Res A. 2004 Jun 01; 69(3):552-60.JB

Abstract

The purpose of this study was to evaluate the efficacy of zinc (Zn)-containing beta-tricalcium phosphate (Zn-TCP) in correcting the bone mineral deficiency noted in osteoporosis using ovariectomized rat model. Four rats were used for each of the four experimental groups: D0, D10, D20, and N10. The rats in D0, D10, and D20 groups were ovariectomized, and fed a vitamin D-, Ca-, and Zn-deficient diet, and induced Zn-deficient osteoporoses for 9 weeks. In contrast, the N10 group was the normal rats fed normal healthy diet for 9 weeks. D0 group was injected with pure beta-TCP suspension, D10 and D20 groups were injected with suspensions containing 10 mg of 10 mol % (6.17 wt % Zn) and 20 mol % (12.05 wt % Zn) Zn-TCP, respectively, and the healthy group, N10 were injected with 10 mol %. Zn-TCP suspensions. Injections were administered intramuscularly in the left thigh once a week in all rats, and fed a vitamin D- and Zn-deficient diet for 9 weeks. The plasma calcium (Ca) and Zn levels, plasma alkaline phosphatase activity (ALP) and bone mineral density (BMD) of the lumbar vertebra and femora were measured. The plasma Zn levels in all the rats were between 1.1 and 2.8 microg/mL. The areas under the curves for the Ca, Zn, and ALP (Ca-AUC, Zn-AUC, and ALP-AUC) levels between 0 and 63 days were calculated. Results for the AUCs were as follows: (1) the Zn-AUCs were in the order of N10 = D20 > D10 > D0; (2) the Ca-AUCs for D0, D10 groups were significantly lower than that for the N10 group; (3) the ALP-AUCs for the D10 and D20 groups were significantly higher than that for the N10 group, and that of the D0 group was in between those. The body weight of D10 and D20 groups significantly increased with time, that of the D0 group increased slightly, and that of the N10 group remained unchanged for the entire experimental period. The BMD of the lumbar vertebrae of the D10 and D20 groups (about 100 mg/cm(2)) was significantly higher than that of the D0 group but lower than that of the N10 group. The BMD of the left femur increased more than that of the right femur with the increase in the amount of Zn in the suspension. The results of this study suggest that the local effect on BMD was more pronounced than the effect on the whole body.

Authors+Show Affiliations

Kobe Pharmaceutical University, Department of Pharmaceutical Technology, Motoyama-Kitamachi 4-19-1, Higashi-Nada, Kobe 658-8558, Japan. m-otsuka@kobepharma-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15127402

Citation

Otsuka, Makoto, et al. "Effect of Controlled Zinc Release On Bone Mineral Density From Injectable Zn-containing Beta-tricalcium Phosphate Suspension in Zinc-deficient Diseased Rats." Journal of Biomedical Materials Research. Part A, vol. 69, no. 3, 2004, pp. 552-60.
Otsuka M, Ohshita Y, Marunaka S, et al. Effect of controlled zinc release on bone mineral density from injectable Zn-containing beta-tricalcium phosphate suspension in zinc-deficient diseased rats. J Biomed Mater Res A. 2004;69(3):552-60.
Otsuka, M., Ohshita, Y., Marunaka, S., Matsuda, Y., Ito, A., Ichinose, N., Otsuka, K., & Higuchi, W. I. (2004). Effect of controlled zinc release on bone mineral density from injectable Zn-containing beta-tricalcium phosphate suspension in zinc-deficient diseased rats. Journal of Biomedical Materials Research. Part A, 69(3), 552-60.
Otsuka M, et al. Effect of Controlled Zinc Release On Bone Mineral Density From Injectable Zn-containing Beta-tricalcium Phosphate Suspension in Zinc-deficient Diseased Rats. J Biomed Mater Res A. 2004 Jun 1;69(3):552-60. PubMed PMID: 15127402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of controlled zinc release on bone mineral density from injectable Zn-containing beta-tricalcium phosphate suspension in zinc-deficient diseased rats. AU - Otsuka,Makoto, AU - Ohshita,Yuko, AU - Marunaka,Sunao, AU - Matsuda,Yoshihia, AU - Ito,Atsuo, AU - Ichinose,Noboru, AU - Otsuka,Kuniko, AU - Higuchi,William I, PY - 2004/5/6/pubmed PY - 2005/2/23/medline PY - 2004/5/6/entrez SP - 552 EP - 60 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 69 IS - 3 N2 - The purpose of this study was to evaluate the efficacy of zinc (Zn)-containing beta-tricalcium phosphate (Zn-TCP) in correcting the bone mineral deficiency noted in osteoporosis using ovariectomized rat model. Four rats were used for each of the four experimental groups: D0, D10, D20, and N10. The rats in D0, D10, and D20 groups were ovariectomized, and fed a vitamin D-, Ca-, and Zn-deficient diet, and induced Zn-deficient osteoporoses for 9 weeks. In contrast, the N10 group was the normal rats fed normal healthy diet for 9 weeks. D0 group was injected with pure beta-TCP suspension, D10 and D20 groups were injected with suspensions containing 10 mg of 10 mol % (6.17 wt % Zn) and 20 mol % (12.05 wt % Zn) Zn-TCP, respectively, and the healthy group, N10 were injected with 10 mol %. Zn-TCP suspensions. Injections were administered intramuscularly in the left thigh once a week in all rats, and fed a vitamin D- and Zn-deficient diet for 9 weeks. The plasma calcium (Ca) and Zn levels, plasma alkaline phosphatase activity (ALP) and bone mineral density (BMD) of the lumbar vertebra and femora were measured. The plasma Zn levels in all the rats were between 1.1 and 2.8 microg/mL. The areas under the curves for the Ca, Zn, and ALP (Ca-AUC, Zn-AUC, and ALP-AUC) levels between 0 and 63 days were calculated. Results for the AUCs were as follows: (1) the Zn-AUCs were in the order of N10 = D20 > D10 > D0; (2) the Ca-AUCs for D0, D10 groups were significantly lower than that for the N10 group; (3) the ALP-AUCs for the D10 and D20 groups were significantly higher than that for the N10 group, and that of the D0 group was in between those. The body weight of D10 and D20 groups significantly increased with time, that of the D0 group increased slightly, and that of the N10 group remained unchanged for the entire experimental period. The BMD of the lumbar vertebrae of the D10 and D20 groups (about 100 mg/cm(2)) was significantly higher than that of the D0 group but lower than that of the N10 group. The BMD of the left femur increased more than that of the right femur with the increase in the amount of Zn in the suspension. The results of this study suggest that the local effect on BMD was more pronounced than the effect on the whole body. SN - 1549-3296 UR - https://www.unboundmedicine.com/medline/citation/15127402/Effect_of_controlled_zinc_release_on_bone_mineral_density_from_injectable_Zn_containing_beta_tricalcium_phosphate_suspension_in_zinc_deficient_diseased_rats_ L2 - https://doi.org/10.1002/jbm.a.30040 DB - PRIME DP - Unbound Medicine ER -