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[Effects of emodin on the intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular mytocytes from guinea pig].
Yao Xue Xue Bao. 2004 Jan; 39(1):5-8.YX

Abstract

AIM

To study the effects of emodin on intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular myocytes from guinea pig.

METHODS

Enzymatic dissociation was used to isolate single ventricular myocytes from adult guinea pig. They were loaded with Ca2(+)-sensitive fluorecent indicator Fluo-3/AM. [Ca2+]i represented by fluorescent intensity (FI) was measured by laser scanning confocal microscope. Whole cell patch clamp technique was used to record ICa-L.

RESULTS

At resting status, [Ca2+]i was not affected by emodin (1-100 mumol.L-1). Emodin at the concentration of 1 mumol.L-1 was shown to increase the [Ca2+]i induced by 60 mmol.L-1 KCl. The peak value of fluorescent intensity was increased from 1,877 +/- 551 to 2,905 +/- 739 (n = 8, P < 0.05). Emodin at the concentration of 10 mumol.L-1 had no effect on the increase of [Ca2+]i induced by 60 mmol.L-1 KCl. However, the increase of [Ca2+]i induced by KCl was reduced to 1,214 +/- 335 (n = 8, P < 0.05) by 100 mumol.L-1 emodin. The density of ICa-L was increased from (-6.2 +/- 1.3) pA/pF to (-8.3 +/- 0.3) pA/pF (n = 6, P < 0.05) by 1 mumol.L-1 emodin at the test pulse of 0 mV. The current was not altered by 10 mumol.L-1 emodin. But it was inhibited from (-6.6 +/- 1.0) pA/pF to (-3.80 +/- 0.16) pA/pF (n = 6, P < 0.05) by 100 mumol.L-1 emodin at the test pulse of +10 mV.

CONCLUSION

Emodin has two-way regulation on [Ca2+]i and ICa-L of cardiomyocytes in guinea pig.

Authors+Show Affiliations

Liu Y
Department of Pharmacology, Harbin Medical University, Harbin 150086, China.
Shan HL
No affiliation info available
Sun HL
No affiliation info available
He SZ
No affiliation info available
Yang BF
No affiliation info available

MeSH

AnimalsCalciumCalcium Channels, L-TypeCell SeparationDose-Response Relationship, DrugEmodinFemaleGuinea PigsHeart VentriclesMaleMyocytes, Cardiac

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15127572

Citation

Liu, Ying, et al. "[Effects of Emodin On the Intracellular Calcium Concentration ([Ca2+]i) and L-type Calcium Current of the Single Ventricular Mytocytes From Guinea Pig]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 39, no. 1, 2004, pp. 5-8.
Liu Y, Shan HL, Sun HL, et al. [Effects of emodin on the intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular mytocytes from guinea pig]. Yao Xue Xue Bao. 2004;39(1):5-8.
Liu, Y., Shan, H. L., Sun, H. L., He, S. Z., & Yang, B. F. (2004). [Effects of emodin on the intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular mytocytes from guinea pig]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 39(1), 5-8.
Liu Y, et al. [Effects of Emodin On the Intracellular Calcium Concentration ([Ca2+]i) and L-type Calcium Current of the Single Ventricular Mytocytes From Guinea Pig]. Yao Xue Xue Bao. 2004;39(1):5-8. PubMed PMID: 15127572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of emodin on the intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular mytocytes from guinea pig]. AU - Liu,Ying, AU - Shan,Hong-li, AU - Sun,Hong-li, AU - He,Shu-zhuang, AU - Yang,Bao-feng, PY - 2004/5/7/pubmed PY - 2004/8/3/medline PY - 2004/5/7/entrez SP - 5 EP - 8 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 39 IS - 1 N2 - AIM: To study the effects of emodin on intracellular calcium concentration ([Ca2+]i) and L-type calcium current of the single ventricular myocytes from guinea pig. METHODS: Enzymatic dissociation was used to isolate single ventricular myocytes from adult guinea pig. They were loaded with Ca2(+)-sensitive fluorecent indicator Fluo-3/AM. [Ca2+]i represented by fluorescent intensity (FI) was measured by laser scanning confocal microscope. Whole cell patch clamp technique was used to record ICa-L. RESULTS: At resting status, [Ca2+]i was not affected by emodin (1-100 mumol.L-1). Emodin at the concentration of 1 mumol.L-1 was shown to increase the [Ca2+]i induced by 60 mmol.L-1 KCl. The peak value of fluorescent intensity was increased from 1,877 +/- 551 to 2,905 +/- 739 (n = 8, P < 0.05). Emodin at the concentration of 10 mumol.L-1 had no effect on the increase of [Ca2+]i induced by 60 mmol.L-1 KCl. However, the increase of [Ca2+]i induced by KCl was reduced to 1,214 +/- 335 (n = 8, P < 0.05) by 100 mumol.L-1 emodin. The density of ICa-L was increased from (-6.2 +/- 1.3) pA/pF to (-8.3 +/- 0.3) pA/pF (n = 6, P < 0.05) by 1 mumol.L-1 emodin at the test pulse of 0 mV. The current was not altered by 10 mumol.L-1 emodin. But it was inhibited from (-6.6 +/- 1.0) pA/pF to (-3.80 +/- 0.16) pA/pF (n = 6, P < 0.05) by 100 mumol.L-1 emodin at the test pulse of +10 mV. CONCLUSION: Emodin has two-way regulation on [Ca2+]i and ICa-L of cardiomyocytes in guinea pig. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/15127572/[Effects_of_emodin_on_the_intracellular_calcium_concentration__[Ca2+]i__and_L_type_calcium_current_of_the_single_ventricular_mytocytes_from_guinea_pig]_ DB - PRIME DP - Unbound Medicine ER -