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Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae.
Free Radic Res. 2004 Mar; 38(3):225-31.FR

Abstract

A soluble protein from Saccharomyces cerevisiae acts as a peroxidase but requires a NADPH-dependent thioredoxin system and was named thioredoxin peroxidase (TPx). The role of TPx in aging of stationary cultures of S. cerevisiae was investigated in a wild-type strain and a mutant yeast strain in which the tsa gene that encodes TPx was disrupted by homologous recombination. The occurrence of oxidative stress during aging of stationary cultures of the yeast has been proposed. Comparison of 5-day-old (young) stationary cultures of S. cerevisiae and of cultures aged for 3 months (old) revealed decreased viability, increased generation of reactive oxygen species, modulation of cellular redox status, and increased cellular oxidative damage reflected by increased protein carbonyl content and lipid peroxidation. The magnitude of this stress was augmented in yeast mutant lacking TPx. These results suggest that TPx may play a direct role in cellular defense against aging of stationary cultures presumably, functioning as an antioxidant enzyme.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, South Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15129730

Citation

Lee, Jin Hyup, and Jeen-Woo Park. "Role of Thioredoxin Peroxidase in Aging of Stationary Cultures of Saccharomyces Cerevisiae." Free Radical Research, vol. 38, no. 3, 2004, pp. 225-31.
Lee JH, Park JW. Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae. Free Radic Res. 2004;38(3):225-31.
Lee, J. H., & Park, J. W. (2004). Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae. Free Radical Research, 38(3), 225-31.
Lee JH, Park JW. Role of Thioredoxin Peroxidase in Aging of Stationary Cultures of Saccharomyces Cerevisiae. Free Radic Res. 2004;38(3):225-31. PubMed PMID: 15129730.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of thioredoxin peroxidase in aging of stationary cultures of Saccharomyces cerevisiae. AU - Lee,Jin Hyup, AU - Park,Jeen-Woo, PY - 2004/5/8/pubmed PY - 2004/9/24/medline PY - 2004/5/8/entrez SP - 225 EP - 31 JF - Free radical research JO - Free Radic Res VL - 38 IS - 3 N2 - A soluble protein from Saccharomyces cerevisiae acts as a peroxidase but requires a NADPH-dependent thioredoxin system and was named thioredoxin peroxidase (TPx). The role of TPx in aging of stationary cultures of S. cerevisiae was investigated in a wild-type strain and a mutant yeast strain in which the tsa gene that encodes TPx was disrupted by homologous recombination. The occurrence of oxidative stress during aging of stationary cultures of the yeast has been proposed. Comparison of 5-day-old (young) stationary cultures of S. cerevisiae and of cultures aged for 3 months (old) revealed decreased viability, increased generation of reactive oxygen species, modulation of cellular redox status, and increased cellular oxidative damage reflected by increased protein carbonyl content and lipid peroxidation. The magnitude of this stress was augmented in yeast mutant lacking TPx. These results suggest that TPx may play a direct role in cellular defense against aging of stationary cultures presumably, functioning as an antioxidant enzyme. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/15129730/Role_of_thioredoxin_peroxidase_in_aging_of_stationary_cultures_of_Saccharomyces_cerevisiae_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760310001649009 DB - PRIME DP - Unbound Medicine ER -