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Leptin deficiency prevents the activation of the murine alpaha 2(I) collagen promoter by acetaldehyde.
Arch Biochem Biophys. 2004 Jun 01; 426(1):73-7.AB

Abstract

Leptin enhances hepatic fibrosis induced experimentally by various agents. The influence of leptin deficiency on murine alpha 2(I) collagen promoter activation by acetaldehyde and by TGF beta 1 was investigated. Acetaldehyde failed to activate the promoter in ob/ob stellate cells as compared to its activating effect in wild-type cells. By contrast, TGF beta 1 increased the activity of the promoter in the ob/ob and wild-type cells. Total cell TGF beta 1 and secretion of total and free TGF beta 1 were lower in cultured stellate cells from ob/ob mice than wild-type mice. Acetaldehyde increased free cell TGF beta1 and secretion of total and free TGF beta 1 in wild-type, but not in ob/ob cells. The lack of activation of the alpha 2(I) collagen promoter by acetaldehyde in association with a lack of increase in TGF beta 1 in response to acetaldehyde in ob/ob stellate cells provides further evidence for a mediating role of TGF beta 1 in the actions of acetaldehyde on collagen transcription.

Authors+Show Affiliations

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205-2195, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15130784

Citation

Potter, James J., et al. "Leptin Deficiency Prevents the Activation of the Murine Alpaha 2(I) Collagen Promoter By Acetaldehyde." Archives of Biochemistry and Biophysics, vol. 426, no. 1, 2004, pp. 73-7.
Potter JJ, Rennie-Tankersley L, Mezey E. Leptin deficiency prevents the activation of the murine alpaha 2(I) collagen promoter by acetaldehyde. Arch Biochem Biophys. 2004;426(1):73-7.
Potter, J. J., Rennie-Tankersley, L., & Mezey, E. (2004). Leptin deficiency prevents the activation of the murine alpaha 2(I) collagen promoter by acetaldehyde. Archives of Biochemistry and Biophysics, 426(1), 73-7.
Potter JJ, Rennie-Tankersley L, Mezey E. Leptin Deficiency Prevents the Activation of the Murine Alpaha 2(I) Collagen Promoter By Acetaldehyde. Arch Biochem Biophys. 2004 Jun 1;426(1):73-7. PubMed PMID: 15130784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leptin deficiency prevents the activation of the murine alpaha 2(I) collagen promoter by acetaldehyde. AU - Potter,James J, AU - Rennie-Tankersley,Lynda, AU - Mezey,Esteban, PY - 2004/02/25/received PY - 2004/03/24/revised PY - 2004/5/8/pubmed PY - 2004/6/16/medline PY - 2004/5/8/entrez SP - 73 EP - 7 JF - Archives of biochemistry and biophysics JO - Arch Biochem Biophys VL - 426 IS - 1 N2 - Leptin enhances hepatic fibrosis induced experimentally by various agents. The influence of leptin deficiency on murine alpha 2(I) collagen promoter activation by acetaldehyde and by TGF beta 1 was investigated. Acetaldehyde failed to activate the promoter in ob/ob stellate cells as compared to its activating effect in wild-type cells. By contrast, TGF beta 1 increased the activity of the promoter in the ob/ob and wild-type cells. Total cell TGF beta 1 and secretion of total and free TGF beta 1 were lower in cultured stellate cells from ob/ob mice than wild-type mice. Acetaldehyde increased free cell TGF beta1 and secretion of total and free TGF beta 1 in wild-type, but not in ob/ob cells. The lack of activation of the alpha 2(I) collagen promoter by acetaldehyde in association with a lack of increase in TGF beta 1 in response to acetaldehyde in ob/ob stellate cells provides further evidence for a mediating role of TGF beta 1 in the actions of acetaldehyde on collagen transcription. SN - 0003-9861 UR - https://www.unboundmedicine.com/medline/citation/15130784/Leptin_deficiency_prevents_the_activation_of_the_murine_alpaha_2_I__collagen_promoter_by_acetaldehyde_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S000398610400178X DB - PRIME DP - Unbound Medicine ER -