TY - JOUR T1 - Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia. AU - Woo,Patrick C Y, AU - Lau,Susanna K P, AU - Wong,Beatrice H L, AU - Tsoi,Hoi-wah, AU - Fung,Ami M Y, AU - Chan,Kwok-hung, AU - Tam,Victoria K P, AU - Peiris,J S Malik, AU - Yuen,Kwok-yung, PY - 2004/5/8/pubmed PY - 2004/9/3/medline PY - 2004/5/8/entrez SP - 2306 EP - 9 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 5 N2 - We report the evaluation of recombinant severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) nucleocapsid protein enzyme-linked immunosorbent assay (ELISA)-based antibody tests for serodiagnosis of SARS-CoV pneumonia and compare the sensitivities and specificities of this ELISA for detection of immunoglobulin G (IgG), IgM, IgA, and their combinations with serum samples from 149 healthy blood donors who donated blood 3 years ago as controls and 106 SARS-CoV pneumonia patients in Hong Kong. The specificities of the ELISA for IgG, IgM, and IgA detection were 95.3, 96.6, and 96.6%, respectively, with corresponding sensitivities of 94.3, 59.4, and 60.4%, respectively. The present ELISA appears to be a sensitive test for serodiagnosis of SARS-CoV pneumonia, is much more economical and less labor-intensive than the indirect immunofluorescence assay, and does not require cultivation of SARS-CoV. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15131220/Detection_of_specific_antibodies_to_severe_acute_respiratory_syndrome__SARS__coronavirus_nucleocapsid_protein_for_serodiagnosis_of_SARS_coronavirus_pneumonia_ DB - PRIME DP - Unbound Medicine ER -