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FcepsilonRI engagement of Langerhans cell-like dendritic cells and inflammatory dendritic epidermal cell-like dendritic cells induces chemotactic signals and different T-cell phenotypes in vitro.
J Allergy Clin Immunol. 2004 May; 113(5):949-57.JA

Abstract

BACKGROUND

Atopic dermatitis (AD) is a biphasic inflammatory skin disease characterized by an initial phase predominated by T(H)2 cytokines, which switches into a second T(H)1-dominated chronic phase. Thus far, the small number of FcepsilonRI-bearing Langerhans cells (LCs) and inflammatory dendritic epidermal cells (IDECs) in the epidermis of patients with AD has hampered a detailed functional analysis and limited our knowledge of these dendritic cells (DCs).

OBJECTIVE

We studied FcepsilonRI-mediated mechanisms of LCs and IDECs with the help of a novel in vitro model.

METHODS

Langerhans cell-like dendritic cells (LC-DCs) and inflammatory dendritic epidermal cell-like dendritic cells (IDEC-DCs) bearing FcepsilonRI have been generated from monocytes of the same atopic donor and compared functionally with LCs and IDECs isolated from the skin of patients with AD.

RESULTS

We found that FcepsilonRI-activated LC-DCs release chemotactic signals, and supernatants of FcepsilonRI-activated LC-DCs increase the migratory capacity of precursor cells of IDECs and naive T cells in vitro. FcepsilonRI-activated IDEC-DCs produce high amounts of proinflammatory cytokines and chemokines and might thereby amplify the inflammatory immune reaction in patients with AD. Furthermore, FcepsilonRI-activated IDEC-DCs prime naive T cells into IFN-gamma-producing T cells and release IL-12 and IL-18, which together might lead to the switch of the initial T(H)2-type immune response into a response of the T(H)1 type in vivo.

CONCLUSION

The present study provides evidence that FcepsilonRI-activated LC-DCs and IDEC-DCs contribute distinctly to the outcome of T-cell responses in vitro and might have implications for the biphasic nature of AD in vivo.

Authors+Show Affiliations

Department of Dermatology, University of Bonn, Sigmund-Freud-Strasse 25, 53105 Bonn, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15131579

Citation

Novak, Natalija, et al. "FcepsilonRI Engagement of Langerhans Cell-like Dendritic Cells and Inflammatory Dendritic Epidermal Cell-like Dendritic Cells Induces Chemotactic Signals and Different T-cell Phenotypes in Vitro." The Journal of Allergy and Clinical Immunology, vol. 113, no. 5, 2004, pp. 949-57.
Novak N, Valenta R, Bohle B, et al. FcepsilonRI engagement of Langerhans cell-like dendritic cells and inflammatory dendritic epidermal cell-like dendritic cells induces chemotactic signals and different T-cell phenotypes in vitro. J Allergy Clin Immunol. 2004;113(5):949-57.
Novak, N., Valenta, R., Bohle, B., Laffer, S., Haberstok, J., Kraft, S., & Bieber, T. (2004). FcepsilonRI engagement of Langerhans cell-like dendritic cells and inflammatory dendritic epidermal cell-like dendritic cells induces chemotactic signals and different T-cell phenotypes in vitro. The Journal of Allergy and Clinical Immunology, 113(5), 949-57.
Novak N, et al. FcepsilonRI Engagement of Langerhans Cell-like Dendritic Cells and Inflammatory Dendritic Epidermal Cell-like Dendritic Cells Induces Chemotactic Signals and Different T-cell Phenotypes in Vitro. J Allergy Clin Immunol. 2004;113(5):949-57. PubMed PMID: 15131579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - FcepsilonRI engagement of Langerhans cell-like dendritic cells and inflammatory dendritic epidermal cell-like dendritic cells induces chemotactic signals and different T-cell phenotypes in vitro. AU - Novak,Natalija, AU - Valenta,Rudolf, AU - Bohle,Barbara, AU - Laffer,Sylvia, AU - Haberstok,Jörg, AU - Kraft,Stefan, AU - Bieber,Thomas, PY - 2004/5/8/pubmed PY - 2004/6/18/medline PY - 2004/5/8/entrez SP - 949 EP - 57 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 113 IS - 5 N2 - BACKGROUND: Atopic dermatitis (AD) is a biphasic inflammatory skin disease characterized by an initial phase predominated by T(H)2 cytokines, which switches into a second T(H)1-dominated chronic phase. Thus far, the small number of FcepsilonRI-bearing Langerhans cells (LCs) and inflammatory dendritic epidermal cells (IDECs) in the epidermis of patients with AD has hampered a detailed functional analysis and limited our knowledge of these dendritic cells (DCs). OBJECTIVE: We studied FcepsilonRI-mediated mechanisms of LCs and IDECs with the help of a novel in vitro model. METHODS: Langerhans cell-like dendritic cells (LC-DCs) and inflammatory dendritic epidermal cell-like dendritic cells (IDEC-DCs) bearing FcepsilonRI have been generated from monocytes of the same atopic donor and compared functionally with LCs and IDECs isolated from the skin of patients with AD. RESULTS: We found that FcepsilonRI-activated LC-DCs release chemotactic signals, and supernatants of FcepsilonRI-activated LC-DCs increase the migratory capacity of precursor cells of IDECs and naive T cells in vitro. FcepsilonRI-activated IDEC-DCs produce high amounts of proinflammatory cytokines and chemokines and might thereby amplify the inflammatory immune reaction in patients with AD. Furthermore, FcepsilonRI-activated IDEC-DCs prime naive T cells into IFN-gamma-producing T cells and release IL-12 and IL-18, which together might lead to the switch of the initial T(H)2-type immune response into a response of the T(H)1 type in vivo. CONCLUSION: The present study provides evidence that FcepsilonRI-activated LC-DCs and IDEC-DCs contribute distinctly to the outcome of T-cell responses in vitro and might have implications for the biphasic nature of AD in vivo. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/15131579/FcepsilonRI_engagement_of_Langerhans_cell_like_dendritic_cells_and_inflammatory_dendritic_epidermal_cell_like_dendritic_cells_induces_chemotactic_signals_and_different_T_cell_phenotypes_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674904009790 DB - PRIME DP - Unbound Medicine ER -