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IRI-1, a LIN-15B homologue, interacts with inositol-1,4,5-triphosphate receptors and regulates gonadogenesis, defecation, and pharyngeal pumping in Caenorhabditis elegans.
Mol Biol Cell. 2004 Jul; 15(7):3073-82.MB

Abstract

Inositol-1,4,5-triphosphate receptors (IP(3)Rs) are ligand-gated Ca(2+) channels that control Ca(2+) release from intracellular stores. They are central to a wide range of cellular responses. IP(3)Rs in Caenorhabditis elegans are encoded by a single gene, itr-1, and are widely expressed. Signaling through IP(3) and IP(3)Rs is important in ovulation, control of the defecation cycle, modulation of pharyngeal pumping rate, and embryogenesis. To further elucidate the molecular basis of the diversity of IP(3)R function, we used a yeast two-hybrid screen to search for proteins that interact with ITR-1. We identified an interaction between ITR-1 and IRI-1, a previously uncharacterized protein with homology to LIN-15B. Iri-1 is widely expressed, and its expression overlaps significantly with that of itr-1. In agreement with this observation, iri-1 functions in known itr-1-mediated processes, namely, upregulation of pharyngeal pumping in response to food and control of the defecation cycle. Knockdown of iri-1 in an itr-1 loss-of-function mutant potentiates some of these effects and sheds light on the signaling pathways that control pharyngeal pumping rate. Knockdown of iri-1 expression also results in a sterile, evl phenotype, as a consequence of failures in early Z1/Z4 lineage divisions, such that gonadogenesis is severely disrupted.

Authors+Show Affiliations

Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15133127

Citation

Walker, Denise S., et al. "IRI-1, a LIN-15B Homologue, Interacts With Inositol-1,4,5-triphosphate Receptors and Regulates Gonadogenesis, Defecation, and Pharyngeal Pumping in Caenorhabditis Elegans." Molecular Biology of the Cell, vol. 15, no. 7, 2004, pp. 3073-82.
Walker DS, Ly S, Gower NJ, et al. IRI-1, a LIN-15B homologue, interacts with inositol-1,4,5-triphosphate receptors and regulates gonadogenesis, defecation, and pharyngeal pumping in Caenorhabditis elegans. Mol Biol Cell. 2004;15(7):3073-82.
Walker, D. S., Ly, S., Gower, N. J., & Baylis, H. A. (2004). IRI-1, a LIN-15B homologue, interacts with inositol-1,4,5-triphosphate receptors and regulates gonadogenesis, defecation, and pharyngeal pumping in Caenorhabditis elegans. Molecular Biology of the Cell, 15(7), 3073-82.
Walker DS, et al. IRI-1, a LIN-15B Homologue, Interacts With Inositol-1,4,5-triphosphate Receptors and Regulates Gonadogenesis, Defecation, and Pharyngeal Pumping in Caenorhabditis Elegans. Mol Biol Cell. 2004;15(7):3073-82. PubMed PMID: 15133127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IRI-1, a LIN-15B homologue, interacts with inositol-1,4,5-triphosphate receptors and regulates gonadogenesis, defecation, and pharyngeal pumping in Caenorhabditis elegans. AU - Walker,Denise S, AU - Ly,Sung, AU - Gower,Nicholas J D, AU - Baylis,Howard A, Y1 - 2004/05/07/ PY - 2004/5/11/pubmed PY - 2005/2/3/medline PY - 2004/5/11/entrez SP - 3073 EP - 82 JF - Molecular biology of the cell JO - Mol Biol Cell VL - 15 IS - 7 N2 - Inositol-1,4,5-triphosphate receptors (IP(3)Rs) are ligand-gated Ca(2+) channels that control Ca(2+) release from intracellular stores. They are central to a wide range of cellular responses. IP(3)Rs in Caenorhabditis elegans are encoded by a single gene, itr-1, and are widely expressed. Signaling through IP(3) and IP(3)Rs is important in ovulation, control of the defecation cycle, modulation of pharyngeal pumping rate, and embryogenesis. To further elucidate the molecular basis of the diversity of IP(3)R function, we used a yeast two-hybrid screen to search for proteins that interact with ITR-1. We identified an interaction between ITR-1 and IRI-1, a previously uncharacterized protein with homology to LIN-15B. Iri-1 is widely expressed, and its expression overlaps significantly with that of itr-1. In agreement with this observation, iri-1 functions in known itr-1-mediated processes, namely, upregulation of pharyngeal pumping in response to food and control of the defecation cycle. Knockdown of iri-1 in an itr-1 loss-of-function mutant potentiates some of these effects and sheds light on the signaling pathways that control pharyngeal pumping rate. Knockdown of iri-1 expression also results in a sterile, evl phenotype, as a consequence of failures in early Z1/Z4 lineage divisions, such that gonadogenesis is severely disrupted. SN - 1059-1524 UR - https://www.unboundmedicine.com/medline/citation/15133127/IRI_1_a_LIN_15B_homologue_interacts_with_inositol_145_triphosphate_receptors_and_regulates_gonadogenesis_defecation_and_pharyngeal_pumping_in_Caenorhabditis_elegans_ L2 - https://www.molbiolcell.org/doi/10.1091/mbc.e04-01-0039?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -