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CD22 attenuates calcium signaling by potentiating plasma membrane calcium-ATPase activity.
Nat Immunol. 2004 Jun; 5(6):651-7.NI

Abstract

Binding of antigen to the B cell receptor induces a calcium response, which is required for proliferation and antibody production. CD22, a B cell surface protein, inhibits this signal through mechanisms that have been obscure. We report here that CD22 augments calcium efflux after B cell receptor crosslinking. Inhibition of plasma membrane calcium-ATPase (PMCA) attenuated these effects, as did disruption by homologous recombination of the gene encoding PMCA4a and PMCA4b. PMCA coimmunoprecipitated with CD22 in an activation-dependent way. CD22 cytoplasmic tyrosine residues were required for association with PMCA and enhancement of calcium efflux. Moreover, CD22 regulation of efflux and the calcium response required the tyrosine phosphatase SHP-1. Thus, SHP-1 and PMCA provide a mechanism by which CD22, a tissue-specific negative regulator, can affect calcium responses.

Authors+Show Affiliations

Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15133509

Citation

Chen, Jie, et al. "CD22 Attenuates Calcium Signaling By Potentiating Plasma Membrane calcium-ATPase Activity." Nature Immunology, vol. 5, no. 6, 2004, pp. 651-7.
Chen J, McLean PA, Neel BG, et al. CD22 attenuates calcium signaling by potentiating plasma membrane calcium-ATPase activity. Nat Immunol. 2004;5(6):651-7.
Chen, J., McLean, P. A., Neel, B. G., Okunade, G., Shull, G. E., & Wortis, H. H. (2004). CD22 attenuates calcium signaling by potentiating plasma membrane calcium-ATPase activity. Nature Immunology, 5(6), 651-7.
Chen J, et al. CD22 Attenuates Calcium Signaling By Potentiating Plasma Membrane calcium-ATPase Activity. Nat Immunol. 2004;5(6):651-7. PubMed PMID: 15133509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD22 attenuates calcium signaling by potentiating plasma membrane calcium-ATPase activity. AU - Chen,Jie, AU - McLean,Paul A, AU - Neel,Benjamin G, AU - Okunade,Gbolahan, AU - Shull,Gary E, AU - Wortis,Henry H, Y1 - 2004/05/09/ PY - 2004/01/06/received PY - 2004/03/16/accepted PY - 2004/5/11/pubmed PY - 2004/6/25/medline PY - 2004/5/11/entrez SP - 651 EP - 7 JF - Nature immunology JO - Nat Immunol VL - 5 IS - 6 N2 - Binding of antigen to the B cell receptor induces a calcium response, which is required for proliferation and antibody production. CD22, a B cell surface protein, inhibits this signal through mechanisms that have been obscure. We report here that CD22 augments calcium efflux after B cell receptor crosslinking. Inhibition of plasma membrane calcium-ATPase (PMCA) attenuated these effects, as did disruption by homologous recombination of the gene encoding PMCA4a and PMCA4b. PMCA coimmunoprecipitated with CD22 in an activation-dependent way. CD22 cytoplasmic tyrosine residues were required for association with PMCA and enhancement of calcium efflux. Moreover, CD22 regulation of efflux and the calcium response required the tyrosine phosphatase SHP-1. Thus, SHP-1 and PMCA provide a mechanism by which CD22, a tissue-specific negative regulator, can affect calcium responses. SN - 1529-2908 UR - https://www.unboundmedicine.com/medline/citation/15133509/CD22_attenuates_calcium_signaling_by_potentiating_plasma_membrane_calcium_ATPase_activity_ L2 - https://doi.org/10.1038/ni1072 DB - PRIME DP - Unbound Medicine ER -