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Hybrid fibres under slow-to-fast transformations: expression is of myosin heavy and light chains in rat soleus muscle.
Pflugers Arch. 2004 Aug; 448(5):507-14.PA

Abstract

The aim of this study was to examine the expression pattern of myosin heavy chain (MHC) and myosin light chain (MLC) isoforms in single fibres from the rat soleus muscle under control (Cont) conditions and under conditions inducing slow-to-fast phenotype transitions. Two models of muscle phenotype modification, namely 2 weeks clenbuterol (CB) administration or hindlimb unloading (HU), were chosen to achieve a full range of appearance of hybrid fibres, i.show $132#e. fibres co-expressing slow and fast myosin isoforms. MHC and MLC compositions were analysed in parallel by one-dimensional-gel electrophoresis. We showed that (i) the slow-to-fast fibre type transitions at the MHC level were accompanied by exchanges of slow with fast MLC isoforms and (ii) that these transitions were characterized by increased proportions of hybrid profiles of both MHC and MLC isoforms, under both CB (27.5%) and HU (18%) conditions when compared with Cont (7%). This suggested a MHC-MLC coordinated program for myosin regulation during fibre type transitions. However, mismatched hybrid co-expression of MHC and MLC was also observed, probably resulting from differences in post-transcriptional regulation. Finally, in all the muscle fibre groups, specific favourable correlations between one MHC (IIa, IId or IIb) and one MLC (regulatory or essential) type were found.

Authors+Show Affiliations

UPRES EA1032, IFR 118, Bat SN4, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq Cedex, France. Laurence.Stevens@univ-lille1.frNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15133670

Citation

Stevens, Laurence, et al. "Hybrid Fibres Under Slow-to-fast Transformations: Expression Is of Myosin Heavy and Light Chains in Rat Soleus Muscle." Pflugers Archiv : European Journal of Physiology, vol. 448, no. 5, 2004, pp. 507-14.
Stevens L, Bastide B, Bozzo C, et al. Hybrid fibres under slow-to-fast transformations: expression is of myosin heavy and light chains in rat soleus muscle. Pflugers Arch. 2004;448(5):507-14.
Stevens, L., Bastide, B., Bozzo, C., & Mounier, Y. (2004). Hybrid fibres under slow-to-fast transformations: expression is of myosin heavy and light chains in rat soleus muscle. Pflugers Archiv : European Journal of Physiology, 448(5), 507-14.
Stevens L, et al. Hybrid Fibres Under Slow-to-fast Transformations: Expression Is of Myosin Heavy and Light Chains in Rat Soleus Muscle. Pflugers Arch. 2004;448(5):507-14. PubMed PMID: 15133670.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hybrid fibres under slow-to-fast transformations: expression is of myosin heavy and light chains in rat soleus muscle. AU - Stevens,Laurence, AU - Bastide,Bruno, AU - Bozzo,Cyril, AU - Mounier,Yvonne, Y1 - 2004/05/07/ PY - 2004/02/23/received PY - 2004/03/23/accepted PY - 2004/5/11/pubmed PY - 2005/2/3/medline PY - 2004/5/11/entrez SP - 507 EP - 14 JF - Pflugers Archiv : European journal of physiology JO - Pflugers Arch VL - 448 IS - 5 N2 - The aim of this study was to examine the expression pattern of myosin heavy chain (MHC) and myosin light chain (MLC) isoforms in single fibres from the rat soleus muscle under control (Cont) conditions and under conditions inducing slow-to-fast phenotype transitions. Two models of muscle phenotype modification, namely 2 weeks clenbuterol (CB) administration or hindlimb unloading (HU), were chosen to achieve a full range of appearance of hybrid fibres, i.show $132#e. fibres co-expressing slow and fast myosin isoforms. MHC and MLC compositions were analysed in parallel by one-dimensional-gel electrophoresis. We showed that (i) the slow-to-fast fibre type transitions at the MHC level were accompanied by exchanges of slow with fast MLC isoforms and (ii) that these transitions were characterized by increased proportions of hybrid profiles of both MHC and MLC isoforms, under both CB (27.5%) and HU (18%) conditions when compared with Cont (7%). This suggested a MHC-MLC coordinated program for myosin regulation during fibre type transitions. However, mismatched hybrid co-expression of MHC and MLC was also observed, probably resulting from differences in post-transcriptional regulation. Finally, in all the muscle fibre groups, specific favourable correlations between one MHC (IIa, IId or IIb) and one MLC (regulatory or essential) type were found. SN - 0031-6768 UR - https://www.unboundmedicine.com/medline/citation/15133670/Hybrid_fibres_under_slow_to_fast_transformations:_expression_is_of_myosin_heavy_and_light_chains_in_rat_soleus_muscle_ L2 - https://dx.doi.org/10.1007/s00424-004-1287-0 DB - PRIME DP - Unbound Medicine ER -