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Serotypes and virulence genes of bovine Shigatoxigenic Escherichia coli (STEC) isolated from a feedlot in Argentina.
Vet Microbiol. 2004 May 20; 100(1-2):3-9.VM

Abstract

Grazing-fed cattle were previously demonstrated to be reservoir of non-O157 Shigatoxigenic Escherichia coli (STEC) serotypes in Argentina. The acid-resistance of some STEC strains makes it reasonable to assume the presence in feedlot of particular STEC serotypes. Fifty-nine animals were sampled every 2 weeks during 6 months by rectal swabs. Twenty-seven of 59 animals (45.8%) were shown to be Stx2(+); 3/59 (5.1%) carried Stx1(+) and 7/59 (11.9%) were Stx1(+) Stx2(+). Among 44 STEC isolates, 31 isolates were associated to 10 O serogroups (O2, O15, O25, O103, O145, O146, O157, O171, O174, O175) and 13 were considered non-typable (NT). Six H antigens (H2, H7, H8, H19, H21, H25) were distributed in 21 isolates whereas 23 were non-mobile (H-). Seventeen of 44 strains (38.6%) were eaeA(+) and 14 (31.8%) harbored the 60MDa plasmid. The megaplasmid (Mp) and eaeA gene were simultaneously found in a limited number of serotypes belonging to the enterohaemorrhagic E. coli (EHEC). E. coli O157:H7 strains, isolated from four (6.8%) animals, corresponded to the Stx2(+), eaeA(+), Mp(+) pattern. Three O157:H7 strains belonged to phage type 4 and the other strain was atypical. Many serotypes isolated from grain-fed cattle (O2:H25, O15:H21, O25:H19, O145:H-, O146:H-, O146:H21, O157:H7, O175:H8) also differed from those isolated by us previously from grazing animals. The serotypes O15:H21, O25:H19 and O175:H8 had not been identified at present as belonging to STEC. This work provides new data for the understanding of the ecology of STEC in grain-fed cattle and confirms that cattle are an important reservoir of STEC.

Authors+Show Affiliations

Laboratorio de Inmunoquímica y Biotecnología, FCV, UNICEN, Pinto 399 (7000) Tandil, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15135507

Citation

Padola, Nora L., et al. "Serotypes and Virulence Genes of Bovine Shigatoxigenic Escherichia Coli (STEC) Isolated From a Feedlot in Argentina." Veterinary Microbiology, vol. 100, no. 1-2, 2004, pp. 3-9.
Padola NL, Sanz ME, Blanco JE, et al. Serotypes and virulence genes of bovine Shigatoxigenic Escherichia coli (STEC) isolated from a feedlot in Argentina. Vet Microbiol. 2004;100(1-2):3-9.
Padola, N. L., Sanz, M. E., Blanco, J. E., Blanco, M., Blanco, J., Etcheverria, A. I., Arroyo, G. H., Usera, M. A., & Parma, A. E. (2004). Serotypes and virulence genes of bovine Shigatoxigenic Escherichia coli (STEC) isolated from a feedlot in Argentina. Veterinary Microbiology, 100(1-2), 3-9.
Padola NL, et al. Serotypes and Virulence Genes of Bovine Shigatoxigenic Escherichia Coli (STEC) Isolated From a Feedlot in Argentina. Vet Microbiol. 2004 May 20;100(1-2):3-9. PubMed PMID: 15135507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes and virulence genes of bovine Shigatoxigenic Escherichia coli (STEC) isolated from a feedlot in Argentina. AU - Padola,Nora L, AU - Sanz,Marcelo E, AU - Blanco,Jesús E, AU - Blanco,Miguel, AU - Blanco,Jorge, AU - Etcheverria,Analía I, AU - Arroyo,Guillermo H, AU - Usera,Miguel A, AU - Parma,Alberto E, PY - 2002/09/10/received PY - 2003/03/04/revised PY - 2003/03/24/accepted PY - 2004/5/12/pubmed PY - 2004/8/25/medline PY - 2004/5/12/entrez SP - 3 EP - 9 JF - Veterinary microbiology JO - Vet Microbiol VL - 100 IS - 1-2 N2 - Grazing-fed cattle were previously demonstrated to be reservoir of non-O157 Shigatoxigenic Escherichia coli (STEC) serotypes in Argentina. The acid-resistance of some STEC strains makes it reasonable to assume the presence in feedlot of particular STEC serotypes. Fifty-nine animals were sampled every 2 weeks during 6 months by rectal swabs. Twenty-seven of 59 animals (45.8%) were shown to be Stx2(+); 3/59 (5.1%) carried Stx1(+) and 7/59 (11.9%) were Stx1(+) Stx2(+). Among 44 STEC isolates, 31 isolates were associated to 10 O serogroups (O2, O15, O25, O103, O145, O146, O157, O171, O174, O175) and 13 were considered non-typable (NT). Six H antigens (H2, H7, H8, H19, H21, H25) were distributed in 21 isolates whereas 23 were non-mobile (H-). Seventeen of 44 strains (38.6%) were eaeA(+) and 14 (31.8%) harbored the 60MDa plasmid. The megaplasmid (Mp) and eaeA gene were simultaneously found in a limited number of serotypes belonging to the enterohaemorrhagic E. coli (EHEC). E. coli O157:H7 strains, isolated from four (6.8%) animals, corresponded to the Stx2(+), eaeA(+), Mp(+) pattern. Three O157:H7 strains belonged to phage type 4 and the other strain was atypical. Many serotypes isolated from grain-fed cattle (O2:H25, O15:H21, O25:H19, O145:H-, O146:H-, O146:H21, O157:H7, O175:H8) also differed from those isolated by us previously from grazing animals. The serotypes O15:H21, O25:H19 and O175:H8 had not been identified at present as belonging to STEC. This work provides new data for the understanding of the ecology of STEC in grain-fed cattle and confirms that cattle are an important reservoir of STEC. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/15135507/Serotypes_and_virulence_genes_of_bovine_Shigatoxigenic_Escherichia_coli__STEC__isolated_from_a_feedlot_in_Argentina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378113503001275 DB - PRIME DP - Unbound Medicine ER -