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Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles.
Fertil Steril. 2004 May; 81(5):1289-95.FS

Abstract

OBJECTIVE

To determine the relationship between sperm chromatin structure assay (SCSA) parameters (DNA fragmentation index [DFI] and high DNA stainability [HDS]), and conventional IVF and IVF/intracytoplasmic sperm injection (ICSI) outcomes.

DESIGN

Retrospective review and prospective study.

SETTING

Private IVF clinic.

PATIENT(S)

Two hundred forty-nine couples undergoing first IVF and/or ICSI cycle.

INTERVENTION(S)

IVF, ICSI, blastocyst culture.

MAIN OUTCOME MEASURE(S)

DFI, HDS, conventional semen parameters, IVF, ICSI.

RESULT(S)

IVF and ICSI fertilization rates were not statistically different between high- and low-DFI groups. More men with > or =15% HDS had lower (<25% and <50%) IVF fertilization rates. High DNA stainability was not related to ICSI fertilization rates. High DNA stainability did not affect blastocyst rates or pregnancy outcomes. Men with > or =30% DFI were at risk for low blastocyst rates (<30%) and no ongoing pregnancies. Men with > or =30% DFI had more male factors. World Health Organization thresholds were not predictive of ongoing pregnancy.

CONCLUSION(S)

The relationship between HDS and poor IVF fertilization rates provides preliminary evidence that ICSI may be indicated in men with > or =15% HDS. Men with high levels of DNA fragmentation (> or =30% DFI) were at greater risk for low blastocyst rates and failure to initiate an ongoing pregnancy. The SCSA provides valuable prognostic information to physicians counseling couples before IVF and/or ICSI cycles.

Authors+Show Affiliations

Markham Fertility Centre, Markham, Ontario, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15136092

Citation

Virro, Michael R., et al. "Sperm Chromatin Structure Assay (SCSA) Parameters Are Related to Fertilization, Blastocyst Development, and Ongoing Pregnancy in in Vitro Fertilization and Intracytoplasmic Sperm Injection Cycles." Fertility and Sterility, vol. 81, no. 5, 2004, pp. 1289-95.
Virro MR, Larson-Cook KL, Evenson DP. Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertil Steril. 2004;81(5):1289-95.
Virro, M. R., Larson-Cook, K. L., & Evenson, D. P. (2004). Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. Fertility and Sterility, 81(5), 1289-95.
Virro MR, Larson-Cook KL, Evenson DP. Sperm Chromatin Structure Assay (SCSA) Parameters Are Related to Fertilization, Blastocyst Development, and Ongoing Pregnancy in in Vitro Fertilization and Intracytoplasmic Sperm Injection Cycles. Fertil Steril. 2004;81(5):1289-95. PubMed PMID: 15136092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles. AU - Virro,Michael R, AU - Larson-Cook,Kjersten L, AU - Evenson,Donald P, PY - 2003/05/14/received PY - 2003/09/15/revised PY - 2003/09/15/accepted PY - 2004/5/12/pubmed PY - 2004/6/25/medline PY - 2004/5/12/entrez SP - 1289 EP - 95 JF - Fertility and sterility JO - Fertil. Steril. VL - 81 IS - 5 N2 - OBJECTIVE: To determine the relationship between sperm chromatin structure assay (SCSA) parameters (DNA fragmentation index [DFI] and high DNA stainability [HDS]), and conventional IVF and IVF/intracytoplasmic sperm injection (ICSI) outcomes. DESIGN: Retrospective review and prospective study. SETTING: Private IVF clinic. PATIENT(S): Two hundred forty-nine couples undergoing first IVF and/or ICSI cycle. INTERVENTION(S): IVF, ICSI, blastocyst culture. MAIN OUTCOME MEASURE(S): DFI, HDS, conventional semen parameters, IVF, ICSI. RESULT(S): IVF and ICSI fertilization rates were not statistically different between high- and low-DFI groups. More men with > or =15% HDS had lower (<25% and <50%) IVF fertilization rates. High DNA stainability was not related to ICSI fertilization rates. High DNA stainability did not affect blastocyst rates or pregnancy outcomes. Men with > or =30% DFI were at risk for low blastocyst rates (<30%) and no ongoing pregnancies. Men with > or =30% DFI had more male factors. World Health Organization thresholds were not predictive of ongoing pregnancy. CONCLUSION(S): The relationship between HDS and poor IVF fertilization rates provides preliminary evidence that ICSI may be indicated in men with > or =15% HDS. Men with high levels of DNA fragmentation (> or =30% DFI) were at greater risk for low blastocyst rates and failure to initiate an ongoing pregnancy. The SCSA provides valuable prognostic information to physicians counseling couples before IVF and/or ICSI cycles. SN - 0015-0282 UR - https://www.unboundmedicine.com/medline/citation/15136092/Sperm_chromatin_structure_assay__SCSA__parameters_are_related_to_fertilization_blastocyst_development_and_ongoing_pregnancy_in_in_vitro_fertilization_and_intracytoplasmic_sperm_injection_cycles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015028204000378 DB - PRIME DP - Unbound Medicine ER -