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Experimental hybrid islet transplantation: application of polyvinyl alcohol membrane for entrapment of islets.
Pancreas. 1992; 7(5):562-8.P

Abstract

In this study, we first examined in vitro a polyvinyl alcohol membrane to be used to contain hybrid islet cells, and second we tested a bioartificial pancreas with entrapment of pancreatic islets in polyvinyl alcohol membrane in rats with experimentally induced diabetes. The permeability of the polyvinyl alcohol membrane to different substances was studied in a two-cell chamber system. Glucose, insulin, and nutrients passed through the membrane easily, whereas the passage of immunoglobulin G was completely prevented, indicating that this membrane could be effective in protecting the bioartificial pancreas from immunorejection. Approximately 2,000 islets collected from three Sprague-Dawley rats were enclosed in a mesh-reinforced polyvinyl alcohol tube and transplanted into the peritoneal cavity of six Wistar rats with streptozotocin-induced diabetes. Their nonfasting serum glucose levels were significantly decreased for at least 12 days. Six diabetic rats receiving intraperitoneal transplantation of free islets without the tube showed a slight but significant decrease in nonfasting serum glucose levels for only 3 days. One diabetic rat with transplantation of the bioartificial pancreas had a significant and sustained decrease in nonfasting glucose levels from pretransplanted levels of 440-500 mg/dl to a mean value of 162 +/- 13 mg/dl for over 3 months without immunosuppression. The bioartificial pancreas was then removed, and glucose levels gradually increased to over 500 mg/dl. The results of the present study suggest that a bioartificial pancreas with entrapment of islets in a polyvinyl alcohol membrane could be a promising therapeutic approach to diabetes mellitus.

Authors+Show Affiliations

First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1513803

Citation

Inoue, K, et al. "Experimental Hybrid Islet Transplantation: Application of Polyvinyl Alcohol Membrane for Entrapment of Islets." Pancreas, vol. 7, no. 5, 1992, pp. 562-8.
Inoue K, Fujisato T, Gu YJ, et al. Experimental hybrid islet transplantation: application of polyvinyl alcohol membrane for entrapment of islets. Pancreas. 1992;7(5):562-8.
Inoue, K., Fujisato, T., Gu, Y. J., Burczak, K., Sumi, S., Kogire, M., Tobe, T., Uchida, K., Nakai, I., & Maetani, S. (1992). Experimental hybrid islet transplantation: application of polyvinyl alcohol membrane for entrapment of islets. Pancreas, 7(5), 562-8.
Inoue K, et al. Experimental Hybrid Islet Transplantation: Application of Polyvinyl Alcohol Membrane for Entrapment of Islets. Pancreas. 1992;7(5):562-8. PubMed PMID: 1513803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental hybrid islet transplantation: application of polyvinyl alcohol membrane for entrapment of islets. A1 - Inoue,K, AU - Fujisato,T, AU - Gu,Y J, AU - Burczak,K, AU - Sumi,S, AU - Kogire,M, AU - Tobe,T, AU - Uchida,K, AU - Nakai,I, AU - Maetani,S, PY - 1992/1/1/pubmed PY - 1992/1/1/medline PY - 1992/1/1/entrez SP - 562 EP - 8 JF - Pancreas JO - Pancreas VL - 7 IS - 5 N2 - In this study, we first examined in vitro a polyvinyl alcohol membrane to be used to contain hybrid islet cells, and second we tested a bioartificial pancreas with entrapment of pancreatic islets in polyvinyl alcohol membrane in rats with experimentally induced diabetes. The permeability of the polyvinyl alcohol membrane to different substances was studied in a two-cell chamber system. Glucose, insulin, and nutrients passed through the membrane easily, whereas the passage of immunoglobulin G was completely prevented, indicating that this membrane could be effective in protecting the bioartificial pancreas from immunorejection. Approximately 2,000 islets collected from three Sprague-Dawley rats were enclosed in a mesh-reinforced polyvinyl alcohol tube and transplanted into the peritoneal cavity of six Wistar rats with streptozotocin-induced diabetes. Their nonfasting serum glucose levels were significantly decreased for at least 12 days. Six diabetic rats receiving intraperitoneal transplantation of free islets without the tube showed a slight but significant decrease in nonfasting serum glucose levels for only 3 days. One diabetic rat with transplantation of the bioartificial pancreas had a significant and sustained decrease in nonfasting glucose levels from pretransplanted levels of 440-500 mg/dl to a mean value of 162 +/- 13 mg/dl for over 3 months without immunosuppression. The bioartificial pancreas was then removed, and glucose levels gradually increased to over 500 mg/dl. The results of the present study suggest that a bioartificial pancreas with entrapment of islets in a polyvinyl alcohol membrane could be a promising therapeutic approach to diabetes mellitus. SN - 0885-3177 UR - https://www.unboundmedicine.com/medline/citation/1513803/Experimental_hybrid_islet_transplantation:_application_of_polyvinyl_alcohol_membrane_for_entrapment_of_islets_ L2 - https://Insights.ovid.com/pubmed?pmid=1513803 DB - PRIME DP - Unbound Medicine ER -