[Determination of aloin in aloes by HPLC].Zhongguo Zhong Yao Za Zhi. 2003 Apr; 28(4):349-51.ZZ
To describe a simple and rapid isocratic reversed-phase high performance liquid chromatography method for the baseline separation, identification and assay of aloin in aloes.
The analytical column was a ZORBAX SB-C18(4.6 mm x 250 mm) filled with a 5 microns stationary phase. The mobile phase consisted of acetonitrile-water (25:75); the flow-rate was 1 mL.min-1. The injection volume was 10 microL. The DAD detector was set at 355 nm.
The calibration curve was linear over the range of 0.17-5.9 micrograms (r = 0.9999). The average recovery of the method was 98.6%, RSD 1.32% (n = 6).
The results showed that this method was reliable and accurate. The method was applied to eleven Cape and East African aloes of different origin.